Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,354,895	G→A	L57L (TTG→TTA)	gltB →	glutamate synthase subunit GltB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,354,895	0	G	A	100.0%	207.1 / NA	59	L57L (TTG→TTA)	gltB	glutamate synthase subunit GltB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/24);  total (35/24)

GTTTCGGCCTGATCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTGCGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTGCTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGC  >  NC_000913/3354762‑3355024                                                                                                                                      |                                                                                                                                  gTTTCGGCCTGATCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTGCGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTa                                                                                                                             >  1:77142/1‑140 (MQ=255)       gCCTGATCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTGCGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACaaaaa                                                                                                                       >  1:335417/1‑140 (MQ=255)            aTCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTGCGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGa                                                                                                                  >  1:571242/1‑140 (MQ=255)            aTCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTGCGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGa                                                                                                                  <  1:519333/140‑1 (MQ=255)            aTCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTGCGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGa                                                                                                                  <  1:461769/140‑1 (MQ=255)            aTCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTGCGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGa                                                                                                                  >  2:461769/1‑140 (MQ=255)            aTCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTGCGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGa                                                                                                                  >  2:519333/1‑140 (MQ=255)              cGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTGCGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATc                                                                                                                <  2:77142/140‑1 (MQ=255)                          aGGCGAACCTAGCCACAAGGTAGTGCGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCa                                                                                                    <  1:704614/140‑1 (MQ=255)                           ggCGAACCTAGCCACAAGGTAGTGCGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCAt                                                                                                   >  1:673319/1‑140 (MQ=255)                             cGAACCTAGCCACAAGGTAGTGCGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCttttt                                                                                                         >  2:1026618/1‑132 (MQ=255)                             cGAACCTAGCCACAAGGTAGTGCGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCttttt                                                                                                         <  1:1026618/132‑1 (MQ=255)                             cGAACCTAGCCACAAGGTAGTGCGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCg                                                                                                 <  1:10154/140‑1 (MQ=255)                               aaCCTAGCCACAAGGTAGTGCGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGtt                                                                                               >  2:387097/1‑140 (MQ=255)                               aaCCTAGCCACAAGGTAGTGCGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGtt                                                                                               >  2:266704/1‑140 (MQ=255)                               aaCCTAGCCACAAGGTAGTGCGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGtt                                                                                               >  1:710665/1‑140 (MQ=255)                               aaCCTAGCCACAAGGTAGTGCGTACTGCAATACACGCACTGGCCCACATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGtt                                                                                               >  1:989883/1‑140 (MQ=255)                                   tAGCCACAAGGTAGTGCGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTgcgc                                                                                           >  1:301827/1‑140 (MQ=255)                                               aGTGCGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTgcgc                                                                                           <  2:854053/128‑1 (MQ=255)                                               aGTGCGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTgcgc                                                                                           >  1:854053/1‑128 (MQ=255)                                                 tGCGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTgg                                                                             >  1:461825/1‑140 (MQ=255)                                                 tGCGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTgg                                                                             >  2:590456/1‑140 (MQ=255)                                                 tGCGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTgg                                                                             >  2:1081325/1‑140 (MQ=255)                                                       cTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTa                                                                       >  2:868008/1‑140 (MQ=255)                                                        tGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGc                                                                                >  2:379137/1‑130 (MQ=255)                                                        tGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGc                                                                                <  1:379137/130‑1 (MQ=255)                                                               cacGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCaaaaaa                                                               >  2:255758/1‑140 (MQ=255)                                                                       ggCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTg                                                       <  1:868008/140‑1 (MQ=255)                                                                        gCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGc                                                         >  2:600911/1‑137 (MQ=255)                                                                        gCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGc                                                         <  1:600911/137‑1 (MQ=255)                                                                         cccGCATGCAGCACCGTGGCGGGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACg                                                          >  2:776112/1‑135 (MQ=255)                                                                         cccGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACg                                                          <  1:776112/135‑1 (MQ=255)                                                                             cATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGa                                                 >  1:228675/1‑140 (MQ=255)                                                                                gcagcaCCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGc                                              >  1:952376/1‑140 (MQ=255)                                                                                  agcaCCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGctc                                            >  1:766986/1‑140 (MQ=255)                                                                                  agcaCCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGctc                                            >  1:445255/1‑140 (MQ=255)                                                                                  agcaCCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGctc                                            <  1:103385/140‑1 (MQ=255)                                                                                     aCCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTc                                         <  1:266704/140‑1 (MQ=255)                                                                                     aCCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTc                                         <  2:389951/140‑1 (MQ=255)                                                                                        gTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTg                                      <  2:989883/140‑1 (MQ=255)                                                                                        gTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTg                                      >  2:839101/1‑140 (MQ=255)                                                                                         tGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGTGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGa                                     >  1:913113/1‑140 (MQ=255)                                                                                         tGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTg                                                       >  2:856416/1‑122 (MQ=255)                                                                                         tGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTg                                                       <  1:856416/122‑1 (MQ=255)                                                                                         tGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGa                                     >  2:996491/1‑140 (MQ=255)                                                                                         tGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGa                                     >  2:975100/1‑140 (MQ=255)                                                                                         tGGCGCGATTCTCGCAGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGa                                     >  1:403696/1‑140 (MQ=255)                                                                                          ggCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGaa                                    >  2:85606/1‑140 (MQ=255)                                                                                             gcgATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATaa                                 <  2:766986/140‑1 (MQ=255)                                                                                             gcgATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATaa                                 <  2:228675/140‑1 (MQ=255)                                                                                              cgATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTc                                         <  1:1055768/131‑1 (MQ=255)                                                                                              cgATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTc                                         >  2:1055768/1‑131 (MQ=255)                                                                                              cgATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATaaa                                >  2:339979/1‑140 (MQ=255)                                                                                                aTTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGa                              >  2:1001772/1‑140 (MQ=255)                                                                                                            ggTAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTcgc                  <  1:130333/140‑1 (MQ=255)                                                                                                              tAAAACCGGCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTcgccg                <  1:339979/140‑1 (MQ=255)                                                                                                                      gCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCa        <  2:461825/140‑1 (MQ=255)                                                                                                                      gCGACGGTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCa        <  2:952376/140‑1 (MQ=255)                                                                                                                            gTTGCGGCTTACTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCAcgccgc  <  2:445255/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                  GTTTCGGCCTGATCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTGCGTACTGCAATACACGCACTGGCCCGCATGCAGCACCGTGGCGCGATTCTCGCCGATGGTAAAACCGGCGACGGTTGCGGCTTGCTGTTACAAAAACCGGATCGCTTTTTTCGCATCGTTGCGCAGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGC  >  NC_000913/3354762‑3355024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑