Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,499,074	A→G	Q416R (CAG→CGG)	cysG →	siroheme synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,499,074	0	A	G	100.0%	293.8 / NA	80	Q416R (CAG→CGG)	cysG	siroheme synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/41);  total (39/41)

GGCGGCAGAAAAACAGACGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCAGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGAACAGTCCATCGCTAATTATTATTGGTCGGGTTGTTGGCCTGCGCGATAAACTGAACTGGTTCTCCAACCATTAATTTAACCCG  >  NC_000913/3498940‑3499210                                                                                                                                       |                                                                                                                                         ggcggcAGAAAAACAGACGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGcgcg                                                                                                                                     <  2:359366/140‑1 (MQ=255)  gcggcAGAAAAACAGACGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGt                                                                                                                                    >  2:923533/1‑140 (MQ=255)    ggcAGAAAAACAGACGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGa                                                                                                                                  <  1:712591/140‑1 (MQ=255)         aaaaaCAGACGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGAc                                                                                                                             <  1:319172/140‑1 (MQ=255)           aaaCAGACGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACgg                                                                                                                           <  2:864901/140‑1 (MQ=255)                  cGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTc                                                                                                                    <  1:470826/140‑1 (MQ=255)                            cTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTgg                                                                                                          <  2:988822/140‑1 (MQ=255)                            cTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTgg                                                                                                          >  1:988822/1‑140 (MQ=255)                             tataTGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTc                                                                                                                    >  2:576656/1‑129 (MQ=255)                             tataTGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTc                                                                                                                    <  1:576656/129‑1 (MQ=255)                                 tGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGaa                                                                                                     >  2:1086125/1‑140 (MQ=255)                                 tGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGaa                                                                                                     >  1:442886/1‑140 (MQ=255)                                    ggTTGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTc                                                                                                                    >  1:225615/1‑122 (MQ=255)                                    ggTTGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTc                                                                                                                    <  2:225615/122‑1 (MQ=255)                                    ggTTGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTg                                                                                                  >  1:1082066/1‑140 (MQ=255)                                    ggTTGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTg                                                                                                  >  2:943213/1‑140 (MQ=255)                                    ggTTGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTg                                                                                                  >  2:352273/1‑140 (MQ=255)                                    ggTTGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTg                                                                                                  >  1:403035/1‑140 (MQ=255)                                    ggTTGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTg                                                                                                  >  1:252696/1‑140 (MQ=255)                                    ggTTGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTg                                                                                                  >  2:958727/1‑140 (MQ=255)                                    ggTTGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCG‑AATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTgg                                                                                                 >  1:472823/1‑140 (MQ=255)                                      ttGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGgc                                                                                                >  2:331185/1‑140 (MQ=255)                                      ttGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGgc                                                                                                >  1:857565/1‑140 (MQ=255)                                      ttGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGgc                                                                                                >  1:332150/1‑140 (MQ=255)                                      ttGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGgc                                                                                                >  1:688702/1‑140 (MQ=255)                                       tGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGgcg                                                                                               >  2:306471/1‑140 (MQ=255)                                       tGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGgcg                                                                                               >  1:1046869/1‑140 (MQ=255)                                       tGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGgcg                                                                                               >  2:303176/1‑140 (MQ=255)                                       tGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGgcg                                                                                               >  2:407607/1‑140 (MQ=255)                                       tGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGgcg                                                                                               >  2:759866/1‑140 (MQ=255)                                        gAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGgcgc                                                                                              <  1:1023265/140‑1 (MQ=255)                                        gAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGgcgc                                                                                              <  2:857565/140‑1 (MQ=255)                                        gAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGgcgc                                                                                              <  1:958727/140‑1 (MQ=255)                                        gAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGgcgc                                                                                              <  2:801812/140‑1 (MQ=255)                                        gAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGgcgc                                                                                              <  1:331185/140‑1 (MQ=255)                                        gAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGgcgc                                                                                              <  2:332150/140‑1 (MQ=255)                                         aaTCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCt                                                                                                            <  2:924602/125‑1 (MQ=255)                                         aaTCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCt                                                                                                            >  1:924602/1‑125 (MQ=255)                                         aaTCAGGCCGCGACTATTCAGCAAAAGCT‑ATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAg                                                                                                              >  2:923721/1‑122 (MQ=255)                                         aaTCAGGCCGCGACTATTCAGCAAAAGCT‑ATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAg                                                                                                              <  1:923721/122‑1 (MQ=255)                                          aTCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCgcag                                                                                            <  2:403035/140‑1 (MQ=255)                                          aTCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCgcag                                                                                            <  1:326269/140‑1 (MQ=255)                                               gCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAAt                                                                                       >  1:671325/1‑140 (MQ=255)                                               gCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAAt                                                                                       >  1:366588/1‑140 (MQ=255)                                                  gcgACTATTCAGCAAAAGCTGATTGAACACTGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGaa                                                                                    <  2:671325/140‑1 (MQ=255)                                                  gcgACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGaa                                                                                    <  2:603376/140‑1 (MQ=255)                                                  gcgACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGaa                                                                                    <  2:438328/140‑1 (MQ=255)                                                  gcgACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGaa                                                                                    <  2:434001/140‑1 (MQ=255)                                                  gcgACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGaa                                                                                    <  2:1046869/140‑1 (MQ=255)                                                  gcgACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGaa                                                                                    <  2:366588/140‑1 (MQ=255)                                                      cTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGAACAGt                                                                                <  1:451131/140‑1 (MQ=255)                                                       tATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGAACAGTc                                                                               <  2:252696/140‑1 (MQ=255)                                                        aTTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGAACAGTcc                                                                              <  1:943213/140‑1 (MQ=255)                                                         ttCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGAACAGTCCa                                                                             <  2:547305/140‑1 (MQ=255)                                                         ttCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGAACAGTCCa                                                                             >  2:595052/1‑140 (MQ=255)                                                         ttCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGAACAGTCCa                                                                             >  2:640207/1‑140 (MQ=255)                                                         ttCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGAACAGTCCa                                                                             >  1:547305/1‑140 (MQ=255)                                                            aGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGAACAGTCCATCg                                                                          <  1:352273/140‑1 (MQ=255)                                                            aGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGAACAGTCCATCg                                                                          <  1:1086125/140‑1 (MQ=255)                                                                   gCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGAACAGTCCATCGCTAatta                                                                   >  2:1075887/1‑140 (MQ=255)                                                                    cTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGAACAGTCCATCGCTAattat                                                                  <  2:103073/140‑1 (MQ=255)                                                                      gATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGAACAGTCCATCGCTAattatta                                                                <  1:923533/140‑1 (MQ=255)                                                                           aaCACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGAACAGTCCATCGCTAATTATTATTGGt                                                           <  1:595052/140‑1 (MQ=255)                                                                             cacGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGAACAGTCCATCGCTAATTATTATTGGTCg                                                         <  1:407607/140‑1 (MQ=255)                                                                                  aaTGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGAACAGTCCATCGCTAATTATTATTGGTCGGgttg                                                    >  2:70815/1‑140 (MQ=255)                                                                                     gCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGAACAGTCCATCGCTAATTATTATTGGTCGGGttgttg                                                 >  2:300530/1‑140 (MQ=255)                                                                                         ggCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGAACAGTCCATCGCTAATTATTATTGGTCGGGTTGTTGGCCt                                             <  1:306471/140‑1 (MQ=255)                                                                                          gCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGAACAGTCCATCGCTAATTATTATTGGTCGGGTTGTTGGCCTg                                            >  1:642890/1‑140 (MQ=255)                                                                                            gAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGAACAGTCCATCGCTAATTATTATTGGTCGGGTTGTTGGCCTgcg                                          >  1:887972/1‑140 (MQ=255)                                                                                                tGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGAACAGTCCATCGCTAATTATTATTGGTCGGGTTGTTGGCCTGCGCGAt                                      >  2:394144/1‑140 (MQ=255)                                                                                                       ggCAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGAACAGTCCATCGCTAATTATTATTGGTCGGGTTGTTGGCCTGCGCGATAaactga                               <  2:1082066/140‑1 (MQ=255)                                                                                                         cAATTGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGAACAGTCCATCGCTAATTATTATTGGTCGGGTTGTTGGCCTGCGCGATAaactgaac                             <  1:1093852/140‑1 (MQ=255)                                                                                                            ttGTCGAAAACGGTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGAACAGTCCATCGCTAATTATTATTGGTCGGGTTGTTGGCCTGCGCGATAAACTGAACTgg                          >  2:440790/1‑140 (MQ=255)                                                                                                                       ggTACGGCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGAACAGTCCATCGCTAATTATTATTGGTCGGGTTGTTGGCCTGCGCGATAAACTGAACTGGTTCTCCAACCa               <  1:300530/140‑1 (MQ=255)                                                                                                                            ggCAGTCACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGAACAGTCCATCGCTAATTATTATTGGTCGGGTTGTTGGCCTGCGCGATAAACTGAACTGGTTCTCCAACCATTAAt          <  2:442886/140‑1 (MQ=255)                                                                                                                                  cACGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGAACAGTCCATCGCTAATTATTATTGGTCGGGTTGTTGGCCTGCGCGATAAACTGAACTGGTTCTCCAACCATTAATTTAAcc    <  1:759866/140‑1 (MQ=255)                                                                                                                                   aCGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGAACAGTCCATCGCTAATTATTATTGGTCGGGTTGTTGGCCTGCGCGATAAACTGAACTGGTTCTCCAACCATTAATTTAAccc   >  1:208351/1‑140 (MQ=255)                                                                                                                                   aCGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGAACAGTCCATCGCTAATTATTATTGGTCGGGTTGTTGGCCTGCGCGATAAACTGAACTGGTTCTCCAACCATTAATTTAAcc    >  1:498546/1‑139 (MQ=255)                                                                                                                                   aCGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGAACAGTCCATCGCTAATTATTATTGGTCGGGTTGTTGGCCTGCGCGATAAACTGAACTGGTTCTCCAACCATTAATTTAAcc    <  2:498546/139‑1 (MQ=255)                                                                                                                                   aCGCGGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGAACAGTCCATCGCTAATTATTATTGGTCGGGTTGTTGGCCTGCGCGAGAAACTGAACTGGTTCTCCAACCATTAATTTAAccc   >  2:754593/1‑140 (MQ=255)                                                                                                                                    cgcgGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGAACAGTCCATCGCTAATTATTATTGGTCGGGTTGTTGGCCTGCGCGATAAACTGAACTGGTTCTCCAACCATTAATTTAACCCg  <  2:887972/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         GGCGGCAGAAAAACAGACGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCAGCGCGTGATTGACGGTACGCTCACACAGCTGGGAGAACTGGCGCAGCAAATGAACAGTCCATCGCTAATTATTATTGGTCGGGTTGTTGGCCTGCGCGATAAACTGAACTGGTTCTCCAACCATTAATTTAACCCG  >  NC_000913/3498940‑3499210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑