Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,521,612 T→C T121A (ACG→GCG)  hofN ← DNA utilization protein HofN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,521,6120TC100.0% 144.4 / NA 40T121A (ACG→GCG) hofNDNA utilization protein HofN
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/15);  total (25/15)

CTCAAATTGCCAGCGTCCCTGCGCATCCTGCTGCGTGGCTCCCCGCTGATTGAGATGAAAAGATGCATCCTGGCGGAGTGACGTTTCTAGTGCGTTTAACGCGGTAATGCTTGTTGTCAGCCCCTTGATCTCCAGCGTTCCCTGCTGCCAGCTTATCGTTGTCAGCCAGGCGTGCTCGGGTAAAAGCGCCGCCAGTGCTTCCAGCGCAGATTGCCAGTCGCGGGTAAATTGTCGCTGGCGCTGCCGTTGCGAACGCTGTTCGCGTA  >  NC_000913/3521475‑3521740
                                                                                                                                         |                                                                                                                                
cTCAAATTGCCAGCGTCCCTGCGCATCCTGCTGCGTGGCTCCCCGCTGATTGAGATGAACAGATGCATCCTGGCGGTGTGACGTTTCTAGTGCGTTTAACGCGGTAATGCTTGTTGTCAGCCCCTTGATCTCCAGCGCTc                                                                                                                                >  2:245718/1‑140 (MQ=255)
cTCAAATTGCCAGCGTCCCTGCGCATCCTGCTGCGTGGCTCCCCGCTGATTGAGATGAAAAGATGCATCCTGGCGGAGTGACGTTTCTAGTGCGTTTAACGCGGTAATGCTTGTTGTCAGCCCCTTGATCTCCAGCGCTc                                                                                                                                >  2:590415/1‑140 (MQ=255)
   aaaTTGCCAGCGTCCCTGCGCATCCTGCTGCGTGGCTCCCCGCTGATTGAGATGAAAAGATGCATCCTGGCGGAGTGACGTTTCTAGTGCGTTTAACGCGGTAATGCTTGTTGTCAGCCCCTTGATCTCCAGCGCTCCct                                                                                                                             >  1:121307/1‑140 (MQ=255)
       tGCCAGCGTCCCTGCGCATCCTGCTGCGTGGCTCCCCGCTGATTGAGATGAAAAGATGCATCCTGGCGGAGTGACGTTTCTAGTGCGTTTAACGCGGTAATGCTTGTTGTCAGCCCCTTGATCTCCAGCGCTCCctgctg                                                                                                                         >  2:914453/1‑140 (MQ=255)
       tGCCAGCGTCCCTGCGCATCCTGCTGCGTGGCTCCCCGCTGATTGAGATGAAAAGATGCATCCTGGCGGAGTGACGTTTCTAGTGCGTTTAACGCGGTAATGCTTGTTGTCAGCCCCTTGATCTCCAGCGCTCCctgctg                                                                                                                         <  1:914453/140‑1 (MQ=255)
             cGTCCCTGCGCATCCTGCTGCGTGGCTCCCCGCTGATTGAGATGAAAAGATGCATCCTGGCGGAGTGACGTTTCTAGTGCGTTTAACGCGGTAATGCTTGTTGTCAGCCCCTTGATCTCCAGCGCTCCCTGCTGCCAGCt                                                                                                                   >  1:777382/1‑140 (MQ=255)
               tCCCTGCGCATCCTGCTGCGTGGCTCCCCGCTGATTGAGATGAAAAGATGCATCCTGGCGGAGTGACGTTTCTAGTGCGTTTAACGCGGTAATGCTTGTTGTCAGCCCCTTGATCTCCAGCGCTCCCTGCTGCCAGCTTa                                                                                                                 <  2:187849/140‑1 (MQ=255)
                   tGCGCATCCTGCTGCGTGGCTCCCCGCTGATTGAGATGAAAAGATGCATCCTGGCGGAGTGACGTTTCTAGTGCGTTTAACGCGGTAATGCTTGTTGTCAGCCCCTTGATCTCCAGCGCTCCCTGCTGCCAGCTTATCGt                                                                                                             >  2:255361/1‑140 (MQ=255)
                    gcgcATCCTGCTGCGTGGCTCCCCGCTGATTGAGATGAAAAGATGCATCCTGGCGGAGTGACGTTTCTAGTGCGTTTAACGCGGTAATGCTTGTTGTCAG‑CCCTTGATCTCCAGCGCTCCCTGCTGCCAGc                                                                                                                    <  1:916433/131‑1 (MQ=255)
                    gcgcATCCTGCTGCGTGGCTCCCCGCTGATTGAGATGAAAAGATGCATCCTGGCGGAGTGACGTTTCTAGTGCGTTTAACGCGGTAATGCTTGTTGTCAG‑CCCTTGATCTCCAGCGCTCCCTGCTGCCAGc                                                                                                                    >  2:916433/1‑131 (MQ=255)
                     cgcATCCTGCTGCGTGGCTCCCCGCTGATTGAGATGAAAAGATGCATCCTGGCGGAGTGACGTTTCTAGTGCGTTTAACGCGGTAATGCTTGTTGTCAGCCCCTTGATCTCCAGCGCTCCCTGCTGCCAGCTTATCGTTg                                                                                                           <  1:651851/140‑1 (MQ=255)
                     cgcATCCTGCTGCGTGGCTCCCCGCTGATTGAGATGAAAAGATGCATCCTGGCGGAGTGACGTTTCTAGTGCGTTTAACGCGGTAATGCTTGTTGTCAGCCCCTTGATCTCCAGCGCTCCCTGCTGCCAGCTTATCGTTg                                                                                                           <  1:490061/140‑1 (MQ=255)
                       cATCCTGCTGCGTGGCTCCCCGCTGATTGAGATGAAAAGATGCATCCTGGCGGAGTGACGTTTCTAGTGCGTTTAACGCGGTAATGCTTGTTGTCAGCCCCTTGATCTCCAGCGCTCCCTGCTGCCAGCTTATCGTTGTc                                                                                                         >  2:360346/1‑140 (MQ=255)
                         tCCTGCTGCGTGGCTCCCCGCTGATTGAGATGAAAAGATGCATCCTGGCGGAGTGACGTTTCTAGTGCGTTTAACGCGGTAATGCTTGTTGTCAGCCCCTTGATCTCCAGCGCTCCCTGCTGCCAGCTTATCGTTGTCAg                                                                                                       <  1:360346/140‑1 (MQ=255)
                          ccTGCTGCGTGGCTCCCCGCTGATTGAGATGAAAAGATGCATCCTGGCGGAGTGACGTTTCTAGTGCGTTTAACGCGGTAATGCTTGTTGTCAGCCCCTTGATCTCCAGCGCTCCCTGCTGCCAGCTTATCGTTGTCAg                                                                                                       >  1:649720/1‑139 (MQ=255)
                          ccTGCTGCGTGGCTCCCCGCTGATTGAGATGAAAAGATGCATCCTGGCGGAGTGACGTTTCTAGTGCGTTTAACGCGGTAATGCTTGTTGTCAGCCCCTTGATCTCCAGCGCTCCCTGCTGCCAGCTTATCGTTGTCAg                                                                                                       <  2:649720/139‑1 (MQ=255)
                          ccTGCTGCGTGGCTCCCCGCTGATTGAGATGAAAAGATGCATCCTGGCGGAGTGACGTTTCTAGTGCGTTTAACGCGGTAATGCTTGTTGTCAGCCCCTTGATCTCCAGCGCTCCCTGCTGCCAGCTTATCGTTGTCAGc                                                                                                      >  2:736672/1‑140 (MQ=255)
                          ccTGCTGCGTGGCTCCCCGCTGATTGAGATGAAAAGATGCATCCTGGCGGAGTGACGTTTCTAGTGCGTTTAACGCGGTAATGCTTGTTGTCAGCCCCTTGATCTCCAGCGCTCCCTGCTGCCAGCTTATCGTTGTCAGc                                                                                                      >  2:879790/1‑140 (MQ=255)
                          ccTGCTGCGTGGCTCCCCGCTGATTGAGATGAAAAGATGCATCCTGGCGGAGTGACGTTTCTAGTGCGTTTAACGCGGTAATGCTTGTTGTCAGCCCCTTGATCTCCAGCGCTCCCTGCTGCCAGCTTATCGTTGTCAGc                                                                                                      >  2:581477/1‑140 (MQ=255)
                            tgctgcGTGGCTCCCCGCTGATTGAGATGAAAAGATGCATCCTGGCGGAGTGACGTTTCTAGTGCGTTTAACGCGGTAATGCTTGTTGTCAGCCCCTTGATCTCCAGCGCTCCCTGCTGCCAGCTTATCGTTGTCAGCCa                                                                                                    >  2:316106/1‑140 (MQ=255)
                                    ggCTCCCCGCTGATTGAGATGAAAAGATGCATCCTGGCGGAGTGACGTTTCTAGTGCGTTTAACGCGGTAATGCTTGTTGTCAGCCCCTTGATCTCCAGCGCTCCCTGCTGCCAGCTTATCGTTGTCAGCCAGGCGTGCt                                                                                            <  1:879790/140‑1 (MQ=255)
                                      cTCCCCGCTGATTGAGATGAAAAGATGCATCCTGGCGGAGTGACGTTTCTAGTGCGTTTAACGCGGTAATGCTTGTTGTCAGCCCCTTGATCTCCAGCGCTCCCTGCTGCCAGCTTATCGTTGTCAGc                                                                                                      >  1:431598/1‑128 (MQ=255)
                                      cTCCCCGCTGATTGAGATGAAAAGATGCATCCTGGCGGAGTGACGTTTCTAGTGCGTTTAACGCGGTAATGCTTGTTGTCAGCCCCTTGATCTCCAGCGCTCCCTGCTGCCAGCTTATCGTTGTCAGc                                                                                                      <  2:431598/128‑1 (MQ=255)
                                                     gaTGAAAAGATGCATCCTGGCGGAGTGACGTTTCTAGTGCGTTTAACGCGGTAATGCTTGTTGTCAGCCCCTTGATCTCCAGCGCTCCCTGCTGCCAGCTTATCGTTGTCAGCCAGGCGTGCTCGGGTAAAAGCgccgcc                                                                           >  2:87773/1‑140 (MQ=255)
                                                      aTGAAAAGATGCATCCTGGCGGAGTGACGTTTCTAGTGCGTTTAACGCGGTAATGCTTGTTGTCAGCCCCTTGATCTCCAGCGCTCCCTGCTGCCAGCTTATCGTTGTCAGCCAGGCGTGCTCGGGTAAAAGCGCCGCCa                                                                          <  2:777382/140‑1 (MQ=255)
                                                               tGCATCCTGGCGGAGTGACGTTTCTAGTGCGTTTAACGCAGTAATGCTTGTTGTCAGCCCCTTGATCTCCAGCGCTCCCTGCTGCTAGCTTATCGTTGTCAGCCAGGCGTGCTCGGGTAAAAGCGCCGCCAGTGCTTCCa                                                                 >  1:506118/1‑140 (MQ=255)
                                                                  aTCCTGGCGGAGTGACGTTTCTAGTGCGTTTAACGCGGTAATGCTTGTTGTCAGCCCCTTGATCTCCAGCGCTCCCTGCTGCCAGCTTATCGTTGTCAGCCAGGCGTGCTCGGGTAAAAGCGCCGCCAGTGCTTCCAgcg                                                              <  1:590415/140‑1 (MQ=255)
                                                                           gAGTGACGTTTCTAGTGCGTTTAACGCGGTAATGCTTGTTGTCAGCCCCTTGATCTCCAGCGCTCCCTGCTGCCAGCTTATCGTTGTCAGCCAGGCGTGCTCGGGTAAAAGCGCCGCCAGTGCTTCCAGCGCAGATTGcc                                                     >  2:618523/1‑140 (MQ=255)
                                                                              tGACGTTTCTAGTGCGTTTAACGCGGTAATGCTTGTTGTCAGCCCCTTGATCTCCAGCGCTCCCTGCTGCCAGCTTATCGTTGTCAGCCAGGCGTGCTCGGGTAAAAGCGCCGCCAGTGCTTCCAGCGCAGATTGCCAGt                                                  <  1:365039/140‑1 (MQ=255)
                                                                                              tttAACGCGGTAATGCTTGTTGTCAGCCCCTTGATCTCCAGCGCTCCCTGCTGCCAGCTTATCGTTGTCAGCCAGGCGTGCTCGGGTAAAAGCGCCGCCAGTGCTTCCAGCGCAGATTGCCAGTCGCGGGTAAATTGTCg                                  >  1:738025/1‑140 (MQ=255)
                                                                                                   cgcgGTAATGCTTGTTGTCAGCCCCTTGATCTCCAGCGCTCCCTGCTGCCAGCTTATCGTTGTCAGCCAGGCGTGCTCGGGTAAAAGCGCCGCCAGTGCTTCCAGCGCAGATTGCCAGTCGCGGGTAAATTGTCGCTGgc                             >  2:315296/1‑140 (MQ=255)
                                                                                                      ggTAATGCTTGTTGTCAGCCCCTTGATCTCCAGCGCTCCCTGCTGCCAGCTTATCGTTGTCAGCCAGGCGTGCTCGGGTAAAAGCGCCGCCAGTGCTTCCAGCGCAGATTGCCAGTCGCGGGTAAATTGTCGCTGgcgc                           >  2:608739/1‑139 (MQ=255)
                                                                                                      ggTAATGCTTGTTGTCAGCCCCTTGATCTCCAGCGCTCCCTGCTGCCAGCTTATCGTTGTCAGCCAGGCGTGCTCGGGTAAAAGCGCCGCCAGTGCTTCCAGCGCAGATTGCCAGTCGCGGGTAAATTGTCGCTGgcgc                           <  1:608739/139‑1 (MQ=255)
                                                                                                         aaTGCTTGTTGTCAGCCCCTTGATCTCCAGCGCTCCCTGCTGCCAGCTTATCGTTGTCAGCCAGGCGTGCTCGGGTAAAAGCGCCGCCAGTGCTTCCAGCGCAGATTGCCAGTCGCGGGTAAATTGTCGCTGGCGCTGcc                       <  1:618523/140‑1 (MQ=255)
                                                                                                                      aGCCCCTTGATCTCCAGCGCTCCCTGCTGCCAGCTTATCGTTGTCAGCCAGGCGTGCTCGGGTAAAAGCGCCGCCAGTGCTTCCAGCGCAGATTGCCAGTCGCGGGTAAATTGTCGCTGGCGCTGCCGTTGCGAACGCTg          >  1:281252/1‑140 (MQ=255)
                                                                                                                      aGCCCCTTGATCTCCAGCGCTCCCTGCTGCCAGCTTATCGTTGTCAGCCAGGCGTGCTCGGGTAAAAGCGCCGCCAGTGCTTCCAGCGCAGATTGCCAGTCGCGGGTAAATTGTCGCTGGCGCTGCCGTTGCGAACGCTg          >  2:692550/1‑140 (MQ=255)
                                                                                                                        ccccTTGATCTCCAGCGCTCCCTGCTGCCAGCTTATCGTTGTCAGCCAGGCGTGCTCGGGTAAAAGCGCCGCCAGTGCTTCCAGCGCAGATTGCCAGTCGCGGGTAAATTGTCGCTGGCGCTGCCGTTGCGAACGCTGtt        >  2:692381/1‑140 (MQ=255)
                                                                                                                        ccccTTGATCTCCAGCGCTCCCTGCTGCCAGCTTATCGTTGTCAGCCAGGCGTGCTCGGGTAAAAGCGCCGCCAGTGCTTCCAGCGCAGATTGCCAGTCGCGGGTAAATTGTCGCTGGCGCTGCCGTTGCGAACGCTGtt        >  2:931771/1‑140 (MQ=255)
                                                                                                                              gATCTCCAGCGCTCCCTGCTGCCAGCTTATCGTTGTCAGCCAGGCGTGCTCGGGTAAAAGCGCCGCCAGTGCTTCCAGCGCAGATTGCCAGTCGCGGGTAAATTGTCGCTGGCGCTGCCGTTGCGaa               >  2:656341/1‑127 (MQ=255)
                                                                                                                              gATCTCCAGCGCTCCCTGCTGCCAGCTTATCGTTGTCAGCCAGGCGTGCTCGGGTAAAAGCGCCGCCAGTGCTTCCAGCGCAGATTGCCAGTCGCGGGTAAATTGTCGCTGGCGCTGCCGTTGCGaa               <  1:656341/127‑1 (MQ=255)
                                                                                                                              gATCTCCAGCGCTCCCTGCTGCCAGCTTATCGTTGTCAGCCAGGCGTGCTCGGGTAAAAGCGCCGCCAGTGCTTCCAGCGCAGATTGCCAGTCGCGGGTAAATTGTCGCTGGCGCTGCCGTTGCGAACGCTGTTCGCGTa  >  2:1012960/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                
CTCAAATTGCCAGCGTCCCTGCGCATCCTGCTGCGTGGCTCCCCGCTGATTGAGATGAAAAGATGCATCCTGGCGGAGTGACGTTTCTAGTGCGTTTAACGCGGTAATGCTTGTTGTCAGCCCCTTGATCTCCAGCGTTCCCTGCTGCCAGCTTATCGTTGTCAGCCAGGCGTGCTCGGGTAAAAGCGCCGCCAGTGCTTCCAGCGCAGATTGCCAGTCGCGGGTAAATTGTCGCTGGCGCTGCCGTTGCGAACGCTGTTCGCGTA  >  NC_000913/3521475‑3521740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑