Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,640,471	T→C	S121P (TCT→CCT)	uspA →	universal stress global stress response regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,640,471	0	T	C	100.0%	328.7 / NA	90	S121P (TCT→CCT)	uspA	universal stress global stress response regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (42/48);  total (42/48)

TGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGTCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGC  >  NC_000913/3640338‑3640605                                                                                                                                      |                                                                                                                                       tGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCg                                                                                                                                  >  2:108361/1‑140 (MQ=255)   cAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCa                                                                                                                                >  2:852974/1‑140 (MQ=255)   cAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCa                                                                                                                                >  1:567334/1‑140 (MQ=255)   cAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCa                                                                                                                                >  2:270214/1‑140 (MQ=255)    aGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCAc                                                                                                                               <  1:665660/140‑1 (MQ=255)         aCCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAg                                                                                                                          <  1:382866/140‑1 (MQ=255)         aCCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAg                                                                                                                          <  1:802275/140‑1 (MQ=255)         aCCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAg                                                                                                                          <  1:395163/140‑1 (MQ=255)         aCCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAg                                                                                                                          <  1:270199/140‑1 (MQ=255)         aCCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAg                                                                                                                          <  1:232613/140‑1 (MQ=255)         aCCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAg                                                                                                                          <  2:827704/140‑1 (MQ=255)          cccAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGc                                                                                                                         >  1:875891/1‑140 (MQ=255)          cccAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGATCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTc                                                                                                                            <  2:750101/137‑1 (MQ=255)          cccAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGATCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTc                                                                                                                            >  1:750101/1‑137 (MQ=255)           ccAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATTTGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCt                                                                                                                        >  2:222503/1‑140 (MQ=255)              aTCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGAt                                                                                                                     <  2:314117/140‑1 (MQ=255)                cACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCa                                                                                                                   >  2:553999/1‑140 (MQ=255)                cACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCa                                                                                                                   >  1:489134/1‑140 (MQ=255)                cACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCa                                                                                                                   >  1:781521/1‑140 (MQ=255)                    gAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAg                                                                                                                          >  2:567439/1‑129 (MQ=255)                    gAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAg                                                                                                                          <  1:567439/129‑1 (MQ=255)                    gAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAacac                                                                                                               >  1:1050534/1‑140 (MQ=255)                     aaaCCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACAcc                                                                                                              >  1:975810/1‑140 (MQ=255)                     aaaCCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACAcc                                                                                                              >  1:877713/1‑140 (MQ=255)                     aaaCCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGA‑TTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAg                                                                                                                          >  1:342383/1‑127 (MQ=255)                     aaaCCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGA‑TTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAg                                                                                                                          <  2:342383/127‑1 (MQ=255)                          cTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCa                                                                                                         <  2:1050534/140‑1 (MQ=255)                          cTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCa                                                                                                         <  2:447518/140‑1 (MQ=255)                           tGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTTGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCa                                                                                                         <  1:938407/139‑1 (MQ=255)                           tGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTTGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCa                                                                                                         >  2:938407/1‑139 (MQ=255)                           tGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCAc                                                                                                        >  2:807919/1‑140 (MQ=255)                             agcggcagcggcGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGt                                                                                                      >  1:624523/1‑140 (MQ=255)                             agcggcagcggcGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGt                                                                                                      >  1:731179/1‑140 (MQ=255)                                ggcagcggcGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGa                                                                                                   <  2:159502/140‑1 (MQ=255)                                 gcagcggcGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGGTATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGat                                                                                                  >  1:1058223/1‑140 (MQ=255)                                   agcggcGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGatat                                                                                                >  2:405550/1‑140 (MQ=255)                                   agcggcGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGatat                                                                                                >  1:634189/1‑140 (MQ=255)                                    gcggcgACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATg                                                                                               <  1:405550/140‑1 (MQ=255)                                      ggcgACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCt                                                                                             <  2:389362/140‑1 (MQ=255)                                       gcgACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTg                                                                                            >  2:107865/1‑140 (MQ=255)                                        cgACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGa                                                                                           <  1:222503/140‑1 (MQ=255)                                                     gTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCt                                                                                             >  1:118384/1‑125 (MQ=255)                                                     gTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCt                                                                                             <  2:118384/125‑1 (MQ=255)                                                       tCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGcgcg                                                                            <  1:553999/140‑1 (MQ=255)                                                        cTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGcga                                                                           <  2:732720/140‑1 (MQ=255)                                                         tGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGcgac                                                                          >  2:995984/1‑140 (MQ=255)                                                          ggTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGcgcg                                                                            <  2:1075474/137‑1 (MQ=255)                                                          ggTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGcgcg                                                                            >  1:1075474/1‑137 (MQ=255)                                                          ggTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGcgacg                                                                         <  1:725701/140‑1 (MQ=255)                                                             cGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACgaag                                                                      <  2:634189/140‑1 (MQ=255)                                                                tGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACgaagaag                                                                   >  2:652217/1‑140 (MQ=255)                                                                tGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACgaagaag                                                                   >  2:853946/1‑140 (MQ=255)                                                                tGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACgaagaag                                                                   >  2:303783/1‑140 (MQ=255)                                                                 gCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACgaagaaga                                                                  <  1:853946/140‑1 (MQ=255)                                                                        aGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCt                                                           <  2:776946/140‑1 (MQ=255)                                                                        aGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCt                                                           <  1:807919/140‑1 (MQ=255)                                                                        aGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCt                                                           <  1:649041/140‑1 (MQ=255)                                                                          aaaTACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTc                                                         >  2:700207/1‑140 (MQ=255)                                                                               cGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTACGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCtt                                                          >  1:588759/1‑134 (MQ=255)                                                                               cGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTACGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCtt                                                          <  2:588759/134‑1 (MQ=255)                                                                               cGATATGGATTCGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCctct                                                    >  1:648996/1‑140 (MQ=255)                                                                                  tatGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTacg                                                 <  1:107865/140‑1 (MQ=255)                                                                                  tatGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTacg                                                 >  2:1034495/1‑140 (MQ=255)                                                                                    tGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTacgac                                               <  1:700207/140‑1 (MQ=255)                                                                                    tGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTacgac                                               <  2:781521/140‑1 (MQ=255)                                                                                    tGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTacgac                                               <  2:624523/140‑1 (MQ=255)                                                                                     ggATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTacgacg                                              <  1:66166/140‑1 (MQ=255)                                                                                     ggATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTacgacg                                              <  1:108361/140‑1 (MQ=255)                                                                                       aTTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACgtg                                            >  1:572871/1‑140 (MQ=255)                                                                                       aTTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACgtg                                            >  1:273589/1‑140 (MQ=255)                                                                                       aTTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACgtg                                            >  1:42662/1‑140 (MQ=255)                                                                                         ttGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTacgac                                               <  2:836515/135‑1 (MQ=255)                                                                                         ttGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTacgac                                               >  1:836515/1‑135 (MQ=255)                                                                                           ggtggtTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTcc                                        >  2:1013804/1‑140 (MQ=255)                                                                                            gtggtTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCt                                       >  1:485367/1‑140 (MQ=255)                                                                                              ggtTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGa                                     <  1:1034495/140‑1 (MQ=255)                                                                                                tttGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAAc                                   <  2:648996/140‑1 (MQ=255)                                                                                                 ttGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACg                                  <  1:594153/140‑1 (MQ=255)                                                                                                   gtgGTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGcc                                <  2:504660/140‑1 (MQ=255)                                                                                                      gTCACCACCAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGc                             <  2:489134/140‑1 (MQ=255)                                                                                                         accaccAGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCata                          >  1:898930/1‑140 (MQ=255)                                                                                                              caGGACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTG‑‑‑GCCCGCATATGCGGGCg                  <  2:875891/140‑1 (MQ=255)                                                                                                                 gACTTCTGGAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCg                  >  1:429588/1‑140 (MQ=255)                                                                                                                        ggAGCAAACTGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCt           >  1:679066/1‑140 (MQ=255)                                                                                                                                 tGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGgcgc  <  2:567334/140‑1 (MQ=255)                                                                                                                                 tGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGgcgc  <  2:485367/140‑1 (MQ=255)                                                                                                                                 tGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGgcgc  <  2:429588/140‑1 (MQ=255)                                                                                                                                 tGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGgcgc  <  2:898930/140‑1 (MQ=255)                                                                                                                                 tGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGgcgc  <  2:94862/140‑1 (MQ=255)                                                                                                                                 tGATGCCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCCGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGgcgc  <  2:1058223/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       TGCAGGCTACCCAATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAGGTTCTGGTCGATGCAATCAAGAAATACGATATGGATTTGGTGGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGTCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATATGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAATCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGC  >  NC_000913/3640338‑3640605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑