| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,658,602 | A→G | K79K (AAA→AAG) | gadE → | DNA‑binding transcriptional activator GadE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,658,602 | 0 | A | G | 96.1% | 168.9 / ‑1.8 | 52 | K79K (AAA→AAG) | gadE | DNA‑binding transcriptional activator GadE |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (25/24); total (26/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01 | |||||||||||
AAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAA‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATCTTCTTGAGATTACTTATGGTAAACACTTGCCCCATAAGAATTCACAATTATGTTTTTCACATAATCAGTTCAAAATTATGC > NC_000913/3658480‑3658738 | aaaCCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGcc < 1:507274/140‑1 (MQ=255) aCCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCta < 1:412527/140‑1 (MQ=255) tCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCtatttat > 1:610034/1‑140 (MQ=255) tCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCtatttat > 2:610267/1‑140 (MQ=255) tATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTa > 1:460516/1‑140 (MQ=255) tCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGc > 2:208007/1‑140 (MQ=255) cTATCACAATCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCaaagaaa > 2:204722/1‑140 (MQ=255) aTCACAATCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGt > 1:796535/1‑140 (MQ=255) cacaATCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTAt > 2:780370/1‑140 (MQ=255) aTCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCaaa > 1:184729/1‑140 (MQ=255) tCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCaaaa > 2:350823/1‑140 (MQ=255) atatCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAAt < 2:460516/140‑1 (MQ=255) atCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTGAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAAtct > 2:836895/1‑140 (MQ=255) atCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAAtct > 2:185313/1‑140 (MQ=255) ccTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATACCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAATCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAAtcttc < 2:1022443/140‑1 (MQ=255) cTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAAtcttct < 2:491580/140‑1 (MQ=255) gatgaAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAAtcttct > 1:755728/1‑137 (MQ=255) gatgaAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATCTTCTTga > 1:482427/1‑140 (MQ=255) gatgaAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATCTTCTTga > 2:388986/1‑140 (MQ=255) gatgaAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATCTTCTTga > 2:322634/1‑140 (MQ=255) gatgaAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATCTTCTTga > 2:111628/1‑140 (MQ=255) gatgaAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAC‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAAtcttct < 2:755728/137‑1 (MQ=255) tgaAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATCTTCTTgaga < 1:251265/140‑1 (MQ=255) aagCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATCTTCTTGAGATTACt < 2:184729/140‑1 (MQ=255) agCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATcttctt > 1:877212/1‑130 (MQ=255) agCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATcttctt < 2:877212/130‑1 (MQ=255) agCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATCTTCTTGAGATTACtt < 1:834877/140‑1 (MQ=255) agCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATCTTCTTGAGATTACtt < 2:1086270/140‑1 (MQ=255) agCGATTAAATTTCTGGAGAAATTAGATGACGAGAGAATTATTGTTTTGGCCCCTTATCACATCAGTAAG‑CTCAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAACGAAAGTATCAAAAATCTTCTTGAGATTACtt < 2:887887/140‑1 (MQ=255) aTTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATCTTCTTGAGATTAc < 2:241819/134‑1 (MQ=255) aTTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATCTTCTTGAGATTAc > 1:241819/1‑134 (MQ=255) aTTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATCTTCTTGAGATTACTTATgg > 2:1052281/1‑140 (MQ=255) tAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATCTTCTTGAGATTACTTATGGTa > 2:1083385/1‑140 (MQ=255) tAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATCTTCTTGAGATTACTTATGGTa < 1:166909/140‑1 (MQ=255) aTTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAAGCTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATCTTCTTGAGATTACTTATGGTaaa > 1:1082605/1‑140 (MQ=255) cTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAAGCTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATCTTCTTGAGATTACTTATGGTAAACACt < 2:1082605/140‑1 (MQ=255) ggAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATCTTCTTGAGATTACTTATGGTAAACACTTGc > 2:381851/1‑140 (MQ=255) ggAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATCTTCTTGAGATTACTTATGGTAAACACTTGc < 1:301219/140‑1 (MQ=255) aTTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATCTTCTTGAGATTACTTATGGTAAACACTTGCCCCATa < 1:184606/140‑1 (MQ=255) aTGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATCTTCTTGAGATTACTTATGGTAAACACTTGCCCCATaa < 2:262295/136‑1 (MQ=255) aTGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATCTTCTTGAGATTACTTATGGTAAACACTTGCCCCATaa > 1:262295/1‑136 (MQ=255) aTGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATCTTCTTGAGATTACTTATGGTAAACACTTGCCCCATAAGAAt > 2:17249/1‑140 (MQ=255) tGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATCTTCTTGAGATTACTTATGGTAAACACTTGCCCCATAAGAAtt < 1:770974/140‑1 (MQ=255) cGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGTAAAGAAAGTATCAAAAATCTTCTTGAGATTACTTATGGTAAACACTTGCCCCATAAGAATTcac > 2:657613/1‑140 (MQ=255) cGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATCTTCTTGAGATTACTTATGGTAAACACTTGCCCCATAAGAATTcac > 1:1007473/1‑140 (MQ=255) agagaATTATTGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATCTTCTTGAGATTACTTATGGTAAACACTTGCCCCATAAGAATTCACaa < 2:971201/140‑1 (MQ=255) attGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATCTTCTTGAGATTACTTATGGTAAACACTTGCCCCATAAGAATTCACAATTATGttt < 1:381851/140‑1 (MQ=255) attGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATCTTCTTGAGATTACTTATGGTAAACACTTGCCCCATAAGAATTCACAATTATGttt < 1:514228/140‑1 (MQ=255) attGTTTTGGCACCTTATCACATCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATCTTCTTGAGATTACTTATGGTAAACACTTGCCCCATAAGAATTCACAATTATGttt < 2:1007473/140‑1 (MQ=255) cacaTCAGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATCTTCTTGAGATTACTTATGGTAAACACTTGCCCCATAAGAATTCACAATTATGTTTTTCACATAATCAGTTCaa < 1:322634/140‑1 (MQ=255) aGTAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATCTTCTTGAGATTACTTATGGTAAACACTTGCCCCATAAGAATTCACAATTATGTTTTTCACATAATCAGTTCAAAATTAt < 1:8985/140‑1 (MQ=255) tAAG‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATCTTCTTGAGATTACTTATGGTAAACACTTGCCCCATAAGAATTCACAATTATGTTTTTCACATAATCAGTTCAAAATTATGc < 2:1029695/140‑1 (MQ=255) | AAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTGATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAA‑CTAAAAGCTAAAGCGCCTATTTATTTTGTTAGCCGCAAAGAAAGTATCAAAAATCTTCTTGAGATTACTTATGGTAAACACTTGCCCCATAAGAATTCACAATTATGTTTTTCACATAATCAGTTCAAAATTATGC > NC_000913/3658480‑3658738 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |