Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	307,306	T→C	D676G (GAC→GGC)	ecpC ←	putative fimbrial usher protein EcpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	307,306	0	T	C	100.0%	259.1 / NA	72	D676G (GAC→GGC)	ecpC	putative fimbrial usher protein EcpC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/37);  total (35/37)

GTAACTGTCGAGTGAGTTTTTGCTGTTCTGTAACTCCACCTCATATCTTCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGTCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGC  >  NC_000913/307173‑307435                                                                                                                                      |                                                                                                                                  gTAACTGTCGAGTGAGTTTTTGCTGTTCTGTAACTCCACCTCATATCTTCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCa                                                                                                                             <  1:722560/140‑1 (MQ=255)    aCTGTCGAGTGAGTTTTTGCTGTTCTGTAACTCCACCTCATATCTTCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGcc                                                                                                                          <  2:764844/140‑1 (MQ=255)     cTGTCGAGTGAGTTTTTGCTGTTCTGTAACTCCACCTCATATCTTCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGccc                                                                                                                         >  1:377483/1‑140 (MQ=255)      tGTCGAGTGAGTTTTTGCTGTTCTGTAACTCCACCTCATATCTTCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCg                                                                                                                        <  1:20712/140‑1 (MQ=255)      tGTCGAGTGAGTTTTTGCTGTTCTGTAACTCCACCTCATATCTTCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGACCTGACCGTCGCCCTCCAGCCCg                                                                                                                        <  1:907319/140‑1 (MQ=255)       gTCGAGTGAGTTTTTGCTGTTCTGTAACTCCACCTCATATCTTCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCgg                                                                                                                       >  1:1055168/1‑140 (MQ=255)        tCGAGTGAGTTTTTGCTGTTCTGTAACTCCACCTCATATCTTCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCg                                                                                                                        <  1:799900/138‑1 (MQ=255)        tCGAGTGAGTTTTTGCTGTTCTGTAACTCCACCTCATATCTTCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCg                                                                                                                        >  2:799900/1‑138 (MQ=255)        tCGAGTGAGTTTTTGCTGTTCTGTAACTCCACCTCATATCTTCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCg                                                                                                                        >  2:343260/1‑138 (MQ=255)        tCGAGTGAGTTTTTGCTGTTCTGTAACTCCACCTCATATCTTCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCg                                                                                                                        <  1:343260/138‑1 (MQ=255)        tCGAGTGAGTTTTTGCTGTTCTGTAACTCCACCTCATATCTTCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGgt                                                                                                                      >  1:1048686/1‑140 (MQ=255)        tCGAGTGAGTTTTTGCTGTTCTGTAACTCCACCTCATATCTTCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGgt                                                                                                                      >  1:362296/1‑140 (MQ=255)         cGAGTGAGTTTTTGCTGTTCTGTAACTCCACCTCATATCTTCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGgtg                                                                                                                     >  1:702278/1‑140 (MQ=255)         cGAGTGAGTTTTTGCTGTTCTGTAACTCCACCTCATATCTTCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGgtg                                                                                                                     >  1:1038386/1‑140 (MQ=255)          gagtgagtTTTTGCTGTTCTGTAACTCCACCTCATATCTTCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGgtgt                                                                                                                    <  2:362296/140‑1 (MQ=255)          gagtgagtTTTTGCTGTTCTGTAACTCCACCTCATATCTTCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGgtgt                                                                                                                    >  1:408943/1‑140 (MQ=255)            gtgagtTTTTGCTGTTCTGTAACTCCACCTCATATCTTCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTg                                                                                                                  <  1:588765/140‑1 (MQ=255)                gtTTTTGCTGTTCTGTAACTCCACCTCATATCTTCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAata                                                                                                              <  2:375387/140‑1 (MQ=255)                gtTTTTGCTGTTCTGTAACTCCACCTCATATCTTCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAata                                                                                                              >  2:833373/1‑140 (MQ=255)                    ttGCTGTTCTGTAACTCCACCTCATATCTTCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCAc                                                                                                          <  2:702278/140‑1 (MQ=255)                        tGTTCTGTAACTCCACCTCATATCTTCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCa                                                                                                      <  2:1055168/140‑1 (MQ=255)                          ttCTGTAACTCCACCTCATATCTTCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGc                                                                                                    <  2:569464/140‑1 (MQ=255)                          ttCTGTAACTCCACCTCATATCTTCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGc                                                                                                    <  2:1038386/140‑1 (MQ=255)                             tGTAACTCCACCTCATATCTTCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGtt                                                                                                 <  1:564317/140‑1 (MQ=255)                               tAACTCCACCTCATATCTTCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGc                                                                                               >  2:495309/1‑140 (MQ=255)                                aaCTCCACCTCATATCTTCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGcc                                                                                              <  2:925329/140‑1 (MQ=255)                                    ccACCTCATATCTTCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCAt                                                                                            <  1:338633/138‑1 (MQ=255)                                    ccACCTCATATCTTCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCAt                                                                                            >  2:338633/1‑138 (MQ=255)                                    ccACCTCATATCTTCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCa                                                                                          >  1:670718/1‑140 (MQ=255)                                      aCCTCATATCTTCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGt                                                                                        >  1:851511/1‑140 (MQ=255)                                         tCATATCTTCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCg                                                                                                   >  1:638882/1‑126 (MQ=255)                                         tCATATCTTCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCg                                                                                                   <  2:638882/126‑1 (MQ=255)                                             atCTTCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTccgcccg                                                                                 >  2:44941/1‑140 (MQ=255)                                                 tCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTgg                                                                             <  2:377483/140‑1 (MQ=255)                                                      aGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGgcagca                                                                        >  2:893615/1‑140 (MQ=255)                                                       gggAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCaa                                                                       <  1:495309/140‑1 (MQ=255)                                                        ggAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAAt                                                                      >  1:275531/1‑140 (MQ=255)                                                          agagagCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGt                                                                    >  1:782602/1‑140 (MQ=255)                                                            agagCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGttt                                                                  >  2:174692/1‑140 (MQ=255)                                                            agagCGGGAGATAGTTACGCTTGCCGTTAAGCGGG‑AAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGtttt                                                                 >  2:457254/1‑140 (MQ=255)                                                             gagCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGtttt                                                                 >  2:772352/1‑140 (MQ=255)                                                             gagCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGtttt                                                                 >  2:1006696/1‑140 (MQ=255)                                                                  ggAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTAccc                                                            >  1:249174/1‑140 (MQ=255)                                                                  ggAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTAccc                                                            >  2:131735/1‑140 (MQ=255)                                                                      aTAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGcc                                                        >  1:900690/1‑140 (MQ=255)                                                                        aGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAg                                                      >  1:1094099/1‑140 (MQ=255)                                                                        aGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAg                                                      >  1:554897/1‑140 (MQ=255)                                                                        aGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAg                                                      >  1:703360/1‑140 (MQ=255)                                                                         gTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGc                                                     <  2:1094099/140‑1 (MQ=255)                                                                          ttACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTGCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGcc                                                    >  2:753949/1‑140 (MQ=255)                                                                          ttACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGcc                                                    <  1:833373/140‑1 (MQ=255)                                                                             cGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGAc                                                 >  1:638367/1‑140 (MQ=255)                                                                              gCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACg                                                >  2:316287/1‑140 (MQ=255)                                                                                ttGCCGTTAAGCGGGAAAGTCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCATCCAGCCCCGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGGCAGCCGACGCt                                              >  2:165451/1‑140 (MQ=255)                                                                                ttGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCt                                              <  2:554897/140‑1 (MQ=255)                                                                                  gCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGTGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGAc                                                 >  1:1009852/1‑135 (MQ=255)                                                                                  gCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGTGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGAc                                                 <  2:1009852/135‑1 (MQ=255)                                                                                       tAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTg                                       <  2:1048686/140‑1 (MQ=255)                                                                                             ggAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCAt                                         <  2:1095137/132‑1 (MQ=255)                                                                                             ggAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCAt                                         >  1:1095137/1‑132 (MQ=255)                                                                                                 aaTCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGt                             <  1:131735/140‑1 (MQ=255)                                                                                                     cGCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGt                         <  2:385090/140‑1 (MQ=255)                                                                                                      gCCCGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTa                        >  2:1050548/1‑140 (MQ=255)                                                                                                       cccGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTAt                       <  1:753949/140‑1 (MQ=255)                                                                                                         cGTTGATTTTGGCGCTGATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTg                     <  2:408943/140‑1 (MQ=255)                                                                                                            tGATTTTGGCGCTGATCTGACCTTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACg                  <  1:772352/140‑1 (MQ=255)                                                                                                                         gATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTccgccg     <  1:316287/140‑1 (MQ=255)                                                                                                                         gATCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTccgccg     <  1:1090223/140‑1 (MQ=255)                                                                                                                           tCTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCcgcg   <  2:249174/140‑1 (MQ=255)                                                                                                                            cTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCgcgc  <  2:638367/140‑1 (MQ=255)                                                                                                                            cTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCgcgc  <  1:915046/140‑1 (MQ=255)                                                                                                                            cTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCgcgc  <  2:900690/140‑1 (MQ=255)                                                                                                                            cTGACCGTCGCCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCgcgc  <  1:457254/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                  GTAACTGTCGAGTGAGTTTTTGCTGTTCTGTAACTCCACCTCATATCTTCCATAGGGAGAGAGCGGGAGATAGTTACGCTTGCCGTTAAGCGGGAAAATCCGCCCGTTGATTTTGGCGCTGATCTGACCGTCGTCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGC  >  NC_000913/307173‑307435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑