Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,782,359	T→C	E68E (GAA→GAG)	cysE ←	serine acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,782,359	0	T	C	100.0%	298.2 / NA	83	E68E (GAA→GAG)	cysE	serine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (42/41);  total (42/41)

GCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCTTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCAAGGTTTTCGTGCTTGAGTAGCGTCGCGTGGTAAAAACTGGCCAGCATT  >  NC_000913/3782225‑3782488                                                                                                                                       |                                                                                                                                  gCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTccac                                                                                                                              <  2:112623/140‑1 (MQ=255)  ccTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTccacc                                                                                                                             <  2:647840/140‑1 (MQ=255)   cTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTccacca                                                                                                                            >  2:709155/1‑140 (MQ=255)      cAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACtt                                                                                                                         >  1:435325/1‑140 (MQ=255)      cAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACtt                                                                                                                         >  1:3652/1‑140 (MQ=255)        aGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCa                                                                                                                       <  2:679430/140‑1 (MQ=255)        aGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCa                                                                                                                       <  1:528428/140‑1 (MQ=255)          gCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACg                                                                                                                     <  2:642085/140‑1 (MQ=255)            aTGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGa                                                                                                                   <  1:301007/140‑1 (MQ=255)                    ccTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGatagcaata                                                                                                           <  1:782939/140‑1 (MQ=255)                     cTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACg                                                                                                                     <  1:150264/129‑1 (MQ=255)                     cTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACg                                                                                                                     >  2:150264/1‑129 (MQ=255)                            tataACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCAt                                                                                                   <  2:435325/140‑1 (MQ=255)                              taACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATaa                                                                                                 <  2:768733/140‑1 (MQ=255)                              taACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATaa                                                                                                 <  1:709155/140‑1 (MQ=255)                                  aacGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTgg                                                                                             <  1:260770/140‑1 (MQ=255)                                    cGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCg                                                                                           >  2:982888/1‑140 (MQ=255)                                     ggggTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGc                                                                                            <  1:805027/138‑1 (MQ=255)                                     ggggTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGc                                                                                            >  2:805027/1‑138 (MQ=255)                                     ggggTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGa                                                                                          >  2:717330/1‑140 (MQ=255)                                      gggTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCg                                                                                           >  2:894609/1‑138 (MQ=255)                                      gggTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCg                                                                                           <  1:894609/138‑1 (MQ=255)                                      gggTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGAt                                                                                         >  1:865654/1‑140 (MQ=255)                                        gTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGa                                                                                       >  2:330301/1‑140 (MQ=255)                                         ttGAGTATTTATCGACTGCCGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCg                                                                                           <  2:509492/135‑1 (MQ=255)                                         ttGAGTATTTATCGACTGCCGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCg                                                                                           >  1:509492/1‑135 (MQ=255)                                                 ttATCGACTGCCGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGTGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGtt                                                                              >  1:103684/1‑140 (MQ=255)                                                 ttATCGACTGCCGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGtt                                                                              >  2:251868/1‑140 (MQ=255)                                                      gACTGCCGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCa                                                                         <  1:1085416/140‑1 (MQ=255)                                                        cTGCCGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGc                                                                       >  1:835040/1‑140 (MQ=255)                                                          gCCGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCAt                                                                     >  1:192494/1‑140 (MQ=255)                                                           ccGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCa                                                                         <  2:588539/135‑1 (MQ=255)                                                           ccGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCa                                                                         >  1:588539/1‑135 (MQ=255)                                                           ccGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATg                                                                    >  1:74188/1‑140 (MQ=255)                                                           ccGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATg                                                                    <  1:689873/140‑1 (MQ=255)                                                           ccGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATg                                                                    >  2:689873/1‑140 (MQ=255)                                                           ccGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATg                                                                    >  1:316699/1‑140 (MQ=255)                                                           ccGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATg                                                                    <  1:961433/140‑1 (MQ=255)                                                           ccGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATg                                                                    >  1:1015778/1‑140 (MQ=255)                                                           ccGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATg                                                                    >  2:1043387/1‑140 (MQ=255)                                                                tCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCt                                                               <  1:998524/140‑1 (MQ=255)                                                                   cgGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAg                                                            >  2:997209/1‑140 (MQ=255)                                                                         cGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACt                                                      >  1:468462/1‑140 (MQ=255)                                                                              cGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCCCTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCa                                                        <  2:1047732/133‑1 (MQ=255)                                                                              cGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCaa                                                 >  2:793176/1‑140 (MQ=255)                                                                              cGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCaa                                                 >  2:168271/1‑140 (MQ=255)                                                                              cGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCaa                                                 >  2:700852/1‑140 (MQ=255)                                                                              cGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAAGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCa                                                        >  1:1047732/1‑133 (MQ=255)                                                                               gCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCAAg                                                <  2:835040/140‑1 (MQ=255)                                                                                ccTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCa                                                        >  2:75708/1‑131 (MQ=255)                                                                                ccTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCa                                                        <  1:75708/131‑1 (MQ=255)                                                                                ccTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCAAgg                                               >  2:890952/1‑140 (MQ=255)                                                                                   gAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCAAGGttt                                            <  2:468462/140‑1 (MQ=255)                                                                                    aaTATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCAAGGtttt                                           <  2:1015778/140‑1 (MQ=255)                                                                                    aaTATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCAAGGtttt                                           <  2:624435/140‑1 (MQ=255)                                                                                        tCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCAAGGTTTTCGTg                                       <  2:343777/140‑1 (MQ=255)                                                                                        tCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCAAGGTTTTCGTg                                       <  2:3652/140‑1 (MQ=255)                                                                                         cacaGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCAAGGTTTTCGTGc                                      <  2:1051918/140‑1 (MQ=255)                                                                                         cacaGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCAAGGTTTTCGTGc                                      <  1:793176/140‑1 (MQ=255)                                                                                           caGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCAAGGTTTTCGTGCtt                                    >  2:535610/1‑140 (MQ=255)                                                                                           caGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCAAGGTTTTCGTGCtt                                    >  1:159667/1‑140 (MQ=255)                                                                                           caGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCAAGGTTTTCGTGCtt                                    <  2:316699/140‑1 (MQ=255)                                                                                           caGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCAAGGTTTTCGTGCtt                                    >  1:532296/1‑140 (MQ=255)                                                                                              gCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCAAGGTTTTCGTGCTTGAg                                 <  1:890952/140‑1 (MQ=255)                                                                                              gCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCAAGGTTTTCGTGCTTGAg                                 >  1:973114/1‑140 (MQ=255)                                                                                                cGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGACTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCAAGGTTTTCGTGCTTGAGTa                               >  2:274801/1‑140 (MQ=255)                                                                                                  cAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCAAGGTTTTCGTGCTTGAGTAGc                             >  1:854432/1‑140 (MQ=255)                                                                                                  cAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCAAGGTTTTCGTGCTTGAGTAGc                             >  2:64903/1‑140 (MQ=255)                                                                                                   agagGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCAAGGTTTTCGTGCTTGAGTAGCg                            >  1:701687/1‑140 (MQ=255)                                                                                                      ggCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCAAGGTTTTCGTGCTTGAGTAGCGTcg                         <  2:192494/140‑1 (MQ=255)                                                                                                       gCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCAAGGTTTTCGTGCTTGAGTAGCGTcgc                        <  1:641413/140‑1 (MQ=255)                                                                                                       gCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCAAGGTTTTCGTGCTTGAGTAGCGTcgc                        <  2:854432/140‑1 (MQ=255)                                                                                                       gCGATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCAAGGTTTTCGTGCTTGAGTAGCGTcgc                        >  2:641413/1‑140 (MQ=255)                                                                                                         gATCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCAAGGTTTTCGTGCTTGAGTAGCGTCGCGt                      <  1:982888/140‑1 (MQ=255)                                                                                                          aTCATTTCCGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCAAGGTTTTCGTGCTTGAGTAGCGTCGCGTg                     >  1:605775/1‑140 (MQ=255)                                                                                                                 ccGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCAAGGTTTTCGTGCTTGAGTAGCGTCGCGTGGTaaaaa              >  2:170861/1‑140 (MQ=255)                                                                                                                 ccGGGTCAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCAAGGTTTTCGTGCTTGAGTAGCGTCGCGTGGTaaaaa              >  2:458035/1‑140 (MQ=255)                                                                                                                       cAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCAAGGTTTTCGTGCTTGAGTAGCGTCGCGTGGTAAAAACTGGcc        >  1:325768/1‑140 (MQ=255)                                                                                                                       cAGCGGCGTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGAGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCAAGGTTTTCGTGCTTGAGTAGCGTCGCGTGGTAAAAACTGGcc        <  2:701687/140‑1 (MQ=255)                                                                                                                           ggcgTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCAAGGTTTTCGTGCTTGAGTAGCGTCGCGTGGTAAAAACTGGCCAGCa    <  2:74188/140‑1 (MQ=255)                                                                                                                           ggcgTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCAAGGTTTTCGTGCTTGAGTAGCGTCGCGTGGTAAAAACTGGCCAGCa    <  1:168271/140‑1 (MQ=255)                                                                                                                           ggcgTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCAAGGTTTTCGTGCTTGAGTAGCGTCGCGTGGTAAAAACTGGCCAGCa    <  1:700852/140‑1 (MQ=255)                                                                                                                             cgTAGGCTTCCTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCAAGGTTTTCGTGCTTGAGTAGCGTCGCGTGGTAAAAACTGGCCAGCAtt  <  2:532296/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                  GCCTGCAAGGCATGAAAACCCTTCAGGTATAACAACGGGGTTGAGTATTTATCGACTGCCGGGTCGCGGGTACGCACCGCCTGAATATCACAGGCCGCAGAGGCGATCATTTCCGGGTCAGCGGCGTAGGCTTCTTCCACCACTTCACGGATAGCAATAGCAGGCATAATTGGCGATGACAGCTTGTTCGCCAGCATGTAGCTCAGTGCACTGCCAAGGTTTTCGTGCTTGAGTAGCGTCGCGTGGTAAAAACTGGCCAGCATT  >  NC_000913/3782225‑3782488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑