Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,874,882	C→T	R93R (CGG→CGA)	dgoR ←	putative DNA‑binding transcriptional regulator DgoR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,874,882	0	C	T	100.0%	192.7 / NA	55	R93R (CGG→CGA)	dgoR	putative DNA‑binding transcriptional regulator DgoR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/32);  total (23/32)

GGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCGAGGTAATTCCACTGGTTACGCGGTGCCACAAACGCCCCGCGATAACGTTTCATTTCAATCAGCCGCTTCGCCATCAGCGAACGGAACA  >  NC_000913/3874746‑3875018                                                                                                                                         |                                                                                                                                         ggTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGt                                                                                                                                       <  1:475502/140‑1 (MQ=255)        aTTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGt                                                                                                                                <  2:644285/140‑1 (MQ=255)          tgttgGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAg                                                                                                                              <  1:467099/140‑1 (MQ=255)           gttgGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGt                                                                                                                             >  2:319851/1‑140 (MQ=255)             tgGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCa                                                                                                                           >  2:807383/1‑140 (MQ=255)             tgGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCa                                                                                                                           >  2:318448/1‑140 (MQ=255)             tgGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCa                                                                                                                           <  1:25058/140‑1 (MQ=255)             tgGCAATCATCTCGCTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCa                                                                                                                           >  2:169453/1‑140 (MQ=255)               gCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCa                                                                                                                           <  2:179931/138‑1 (MQ=255)               gCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCa                                                                                                                           >  1:179931/1‑138 (MQ=255)                   tcatcTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTcc                                                                                                                     >  1:42089/1‑140 (MQ=255)                    catcTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCa                                                                                                                    <  2:61402/140‑1 (MQ=255)                      tctcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGc                                                                                                                  <  1:276309/140‑1 (MQ=255)                      tctcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGc                                                                                                                  <  1:1053651/140‑1 (MQ=255)                      tctcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGc                                                                                                                  <  2:1071326/140‑1 (MQ=255)                           ttCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCa                                                                                                             <  1:129300/140‑1 (MQ=255)                                     aTTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACg                                                                                                   >  1:985059/1‑140 (MQ=255)                                      ttCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGt                                                                                                  <  1:318448/140‑1 (MQ=255)                                              gcgcCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCg                                                                                          <  1:99479/140‑1 (MQ=255)                                                     aTCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCGAGGTAAt                                                                                   >  1:413643/1‑140 (MQ=255)                                                       cGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCGAGGTAATTc                                                                                 <  1:874439/140‑1 (MQ=255)                                                         cTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCGAGGTAATTCCa                                                                               <  2:413643/140‑1 (MQ=255)                                                              aGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCGAGGt                                                                                      <  1:565677/128‑1 (MQ=255)                                                              aGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCGAGGt                                                                                      >  2:565677/1‑128 (MQ=255)                                                                  gcgcgcTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCGAGGTAATTCCACTGGTTAcg                                                                      >  1:1045515/1‑140 (MQ=255)                                                                    gcgcTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCGAGGTAATTCCACTGGTTAcgcg                                                                    <  2:985059/140‑1 (MQ=255)                                                                      gcTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCCGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGCACTCATTGCAGTACGTCAGTGTCGAGGTAATTCCACTGGTTACGCGGt                                                                  <  2:753217/140‑1 (MQ=255)                                                                                   aCGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCGGCACCCATTGCAGTACGTCAGTGTCGAGGTAATTCCACTGGTTACGCGGTGCCACAAACGccc                                                     <  1:1033623/140‑1 (MQ=255)                                                                                   aCGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCGAGGTAATTCCACTGGTTACGCGGTGCCACAAACGccc                                                     <  1:319851/140‑1 (MQ=255)                                                                                    cGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCGAGGTAATTCCACTGGTTACGCGGTGCCACAAACGcccc                                                    >  1:686243/1‑140 (MQ=255)                                                                                    cGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCGAGGTAATTCCACTGGTTACGCGGTGCCACAAACGcccc                                                    >  1:15697/1‑140 (MQ=255)                                                                                    cGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCGAGGTAATTCCACTGGTTACGCGGTGCCACAAACGcccc                                                    >  1:176238/1‑140 (MQ=255)                                                                                    cGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCGAGGTAATTCCACTGGTTACGCGGTGCCACAAACGcccc                                                    >  2:340829/1‑140 (MQ=255)                                                                                    cGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCGAGGTAATTCCACTGGTTACGCGGTGCCACAAACGcccc                                                    >  1:619880/1‑140 (MQ=255)                                                                                      ggCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCGAGGTAATTCCACTGGTTACGCGGTGCCACAAACGCCCcgc                                                  <  2:42089/140‑1 (MQ=255)                                                                                        cAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCGAGGTAATTCCACTGGTTACGCGGTGCCACAAACGCCCCGCGa                                                <  2:15697/140‑1 (MQ=255)                                                                                        cAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCGAGGTAATTCCACTGGTTACGCGGTGCCACAAACGCCCCGCGa                                                <  1:169453/140‑1 (MQ=255)                                                                                         aaTCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCGAGGTAATTCCACTGGTTACGCGGTGCCACAAACGCCCCGCGAt                                               <  2:686243/140‑1 (MQ=255)                                                                                          aTCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCGAGGTAATTCCACTGGTTACGCGGTGCCACAAACGCCCCGCGATa                                              <  1:573609/140‑1 (MQ=255)                                                                                               cGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCGAGGTAATTCCACTGGTTACGCGGTGCCACAAACGCCCCGCGATAACGtt                                         <  2:861125/140‑1 (MQ=255)                                                                                                 gTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCGAGGTAATTCCACTGGTTACGCGGTGCCACAAACGCCCCGCGATAACGtttc                                       >  1:28280/1‑140 (MQ=255)                                                                                                 gTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCGAGGTAATTCCACTGGTTACGCGGTGCCACAAACGCCCCGCGATAACGtttc                                       >  2:223977/1‑140 (MQ=255)                                                                                                           aTTTCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCGAGGTAATTCCACTGGTTACGCGGTGCCACAAACGCCCCGCGATAACGTTTCATTTCAATCa                             <  2:983838/140‑1 (MQ=255)                                                                                                            tttCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCGAGGTAATTCCACTGGTTACGCGGTGCCACAAACGCCCCGCGATAACGTTTCATTTCAATCAg                            >  2:3453/1‑140 (MQ=255)                                                                                                             ttCGCACTTCGCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCGAGGTAATTCCACTGGTTACGCGGTGCCACAAACGCCCCGCGATAACGTTTCATTTCAATCAGc                           >  2:380625/1‑140 (MQ=255)                                                                                                                       gCTCATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCGAGGTAATTCCACTGGTTACGCGGTGCCACAAACGCCCCGCGATAACGTTTCATTTCAATCAGCCGCTTCGCCa                 <  2:28280/140‑1 (MQ=255)                                                                                                                          cATGGCACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCGAGGTAATTCCACTGGTTACGCGGTGCCACAAACGCCCCGCGATAACGTTTCATTTCAATCAGCCGCTTCGCCATCa              >  2:35899/1‑140 (MQ=255)                                                                                                                               cACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCGAGGTAATTCCACTGGTTACGCGGTGCCACAAACGCCCCGCGATAACGTTTCATTTCAATCAGCCGCTTCGCCATCAGCGaa         <  1:340829/140‑1 (MQ=255)                                                                                                                               cACTGATAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCGAGGTAATTCCACTGGTTACGCGGTGCCACAAACGCCCCGCGATAACGTTTCATTTCAATCAGCCGCTTCGCCATCAGCGaa         <  1:3453/140‑1 (MQ=255)                                                                                                                                aCTGATAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCGAGGTAATTCCACTGGTTACGCGGTGCCACAAACGCCCCGCGATAACGTTTCATTTCAATCAGCCGCTTCGCCATCAGCGAAc        >  1:30899/1‑140 (MQ=255)                                                                                                                                  tGATAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCGAGGTAATTCCACTGGTTACGCGGTGCCACAAACGCCCCGCGATAACGTTTCATTTCAATCAGCCGCTTCGCCATCAGCGAACgg      >  2:575203/1‑140 (MQ=255)                                                                                                                                   gATAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCGAGGTAATTCCACTGGTTACGCGGTGCCACAAACGCCCCGCGATAACGTTTCATTTCAATCAGCCGCTTCGCCATCAGCGAACGGa     >  2:148567/1‑140 (MQ=255)                                                                                                                                    aTAAGTCGTGGGTCGTAGTCATTTTCCAGCACTCATTGCACTACGTCAGTGTCGAGGTAATTCCACTGGTTACGCGGTGCCACAAACGCCCCGCGATAACGTCTCATTTCAATCAGCCGCTTCGCCATCAGCGAACGGaa    <  2:966704/140‑1 (MQ=255)                                                                                                                                    aTAAGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCGAGGTAATTCCACTGGTTACGCGGTGCCACAAACGCCCCGCGATAACGTTTCATTTCAATCAGCCGCTTCGCCATCAGCGAACGGaa    <  1:704059/140‑1 (MQ=255)                                                                                                                                      aaGTCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCGAGGTAATTCCACTGGTTACGCGGTGCCACAAACGCCCCGCGATAACGTTTCATTTCAATCAGCCGCTTCGCCATCAGCGAACGGAaca  <  1:35899/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         GGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCAGTACGTCAGTGTCGAGGTAATTCCACTGGTTACGCGGTGCCACAAACGCCCCGCGATAACGTTTCATTTCAATCAGCCGCTTCGCCATCAGCGAACGGAACA  >  NC_000913/3874746‑3875018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑