Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,961,491	A→G	K272R (AAG→AGG)	rep →	ATP‑dependent DNA helicase Rep

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,961,491	0	A	G	100.0%	234.7 / NA	65	K272R (AAG→AGG)	rep	ATP‑dependent DNA helicase Rep
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/38);  total (27/38)

TGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTATGGCGCGGAGCTAAAAGTAT  >  NC_000913/3961356‑3961619                                                                                                                                        |                                                                                                                                 tGAAACTGCTGGTGGGCATCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCtt                                                                                                                              >  2:100399/1‑140 (MQ=255) tGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCtt                                                                                                                              <  2:704521/140‑1 (MQ=255)          tggtggGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAAc                                                                                                                     >  2:839241/1‑140 (MQ=255)           ggtggGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACt                                                                                                                    >  2:937485/1‑140 (MQ=255)             tggGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAg                                                                                                                        >  2:950470/1‑134 (MQ=255)             tggGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAg                                                                                                                        <  1:950470/134‑1 (MQ=255)              gggCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATc                                                                                                                 >  2:287289/1‑140 (MQ=255)              gggCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATc                                                                                                                 <  2:581881/140‑1 (MQ=255)               ggCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCg                                                                                                                >  2:934935/1‑140 (MQ=255)                       cgcgcgcTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCg                                                                                                        >  2:179699/1‑140 (MQ=255)                         cgcgcTTTAACGTGGTGGGTGACGATGACCAGTCGCTCTACTCCTGGCGCGGGGCACGTCCGCCAAACCTGGTGCTGTTGAGTCAGGATTTTCCGGCGCTGAACATGATTACGCTTGTGCAGAACTATCGCTCTTCCgtg                                                                                                      >  2:670921/1‑138 (MQ=255)                          gcgcTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGc                                                                                                     <  1:934935/140‑1 (MQ=255)                           cgcTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCg                                                                                                    <  2:123674/140‑1 (MQ=255)                           cgcTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCg                                                                                                    <  1:839241/140‑1 (MQ=255)                           cgcTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCg                                                                                                    <  1:737881/140‑1 (MQ=255)                            gcTTTACCGTGGTGGGTGACGATGACCAGTGGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGt                                                                                                   <  1:670921/140‑1 (MQ=255)                            gcTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGt                                                                                                   <  1:773638/140‑1 (MQ=255)                              tttACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTAt                                                                                                 <  1:100399/140‑1 (MQ=255)                                      ggtgggtgACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAg                                                                                         >  1:571207/1‑140 (MQ=255)                                        tgggtgACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAgcg                                                                                       >  2:442063/1‑140 (MQ=255)                                          ggtgACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAgcggc                                                                                     >  1:168861/1‑140 (MQ=255)                                           gtgACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAgcggcg                                                                                    <  2:619279/140‑1 (MQ=255)                                           gtgACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAgcggcg                                                                                    <  2:213567/140‑1 (MQ=255)                                            tgACGATGACCAGTCGATCTACTCCTGGCGCGGTGCATGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGa                                                                                   >  1:42039/1‑140 (MQ=255)                                            tgACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGa                                                                                   >  2:734563/1‑140 (MQ=255)                                             gACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGaa                                                                                  >  1:1054881/1‑140 (MQ=255)                                                 aTGACCAGTCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATc                                                                              <  2:571207/140‑1 (MQ=255)                                                  tGACCAGTCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTg                                                                                             <  2:880089/124‑1 (MQ=255)                                                  tGACCAGTCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTg                                                                                             >  1:880089/1‑124 (MQ=255)                                                     ccAGTCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGa                                                                          >  2:492721/1‑140 (MQ=255)                                                       aGTCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATc                                                                        <  1:287289/140‑1 (MQ=255)                                                        gTCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGAt                                                                         >  1:741406/1‑138 (MQ=255)                                                        gTCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGAt                                                                         <  2:741406/138‑1 (MQ=255)                                                         tCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATcc                                                                             <  2:701335/133‑1 (MQ=255)                                                         tCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATcc                                                                             >  1:701335/1‑133 (MQ=255)                                                         tCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGAt                                                                         <  1:1051738/137‑1 (MQ=255)                                                         tCGATCTACTCCTGCCGCGGAGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGAt                                                                         >  2:1051738/1‑137 (MQ=255)                                                          cGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGcc                                                                     <  2:434354/140‑1 (MQ=255)                                                                aCTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGAt                                                                         <  1:505389/130‑1 (MQ=255)                                                                aCTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGAt                                                                         >  2:505389/1‑130 (MQ=255)                                                                aCTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAAc                                                               >  2:115529/1‑140 (MQ=255)                                                                aCTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAAc                                                               >  2:622438/1‑140 (MQ=255)                                                                   ccTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGAt                                                                         >  2:6494/1‑127 (MQ=255)                                                                   ccTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGAt                                                                         <  1:6494/127‑1 (MQ=255)                                                                     tGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCa                                                          <  1:819695/140‑1 (MQ=255)                                                                     tGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCa                                                          <  1:442063/140‑1 (MQ=255)                                                                       gcgcGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACg                                                        >  2:511951/1‑140 (MQ=255)                                                                       gcgcGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACg                                                        >  2:614548/1‑140 (MQ=255)                                                                       gcgcGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACg                                                        <  1:179699/140‑1 (MQ=255)                                                                       gcgcGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACg                                                        <  2:539988/140‑1 (MQ=255)                                                                            gTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCttt                                                   >  1:702076/1‑140 (MQ=255)                                                                               cACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGaa                                                <  1:617308/140‑1 (MQ=255)                                                                                aCGTCCGCAAAACCTGGTGCTGCTGGGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGaaa                                               <  1:501070/140‑1 (MQ=255)                                                                                   tCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGAAAAGc                                            <  1:826363/140‑1 (MQ=255)                                                                                     cGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGAAAAGCGt                                          <  2:168861/140‑1 (MQ=255)                                                                                     cGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGAAAAGCGt                                          <  1:622819/140‑1 (MQ=255)                                                                                       cAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGAAAAGCGTCt                                        <  1:937485/140‑1 (MQ=255)                                                                                       cAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGAAAAGCGTCt                                        >  1:4556/1‑140 (MQ=255)                                                                                       cAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGAAAAGCGTCt                                        >  2:919228/1‑140 (MQ=255)                                                                                                 tgctgcTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGaa                              <  2:42039/140‑1 (MQ=255)                                                                                                    tgctgAGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTg                           <  1:622438/140‑1 (MQ=255)                                                                                                         aGTCAGGATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTa                      <  1:199797/140‑1 (MQ=255)                                                                                                              ggATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTATGgcg                 <  2:625030/140‑1 (MQ=255)                                                                                                               gATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTATGgcgc                <  2:702076/140‑1 (MQ=255)                                                                                                               gATTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTATGgcgc                <  1:436643/140‑1 (MQ=255)                                                                                                                aTTTTCCGGCGCTGAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTATGGcgcg               >  2:625304/1‑140 (MQ=255)                                                                                                                             gAAGGTGATTAGGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTATGGCGCGGAGCTAAAAGTAt  <  1:115529/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                 TGAAACTGCTGGTGGGCAGCCGCGCGCGCTTTACCGTGGTGGGTGACGATGACCAGTCGATCTACTCCTGGCGCGGTGCACGTCCGCAAAACCTGGTGCTGCTGAGTCAGGATTTTCCGGCGCTGAAGGTGATTAAGCTTGAGCAGAACTATCGCTCTTCCGGGCGTATTCTGAAAGCGGCGAACATCCTGATCGCCAATAACCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTATGGCGCGGAGCTAAAAGTAT  >  NC_000913/3961356‑3961619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑