Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,027,317	A→G	intergenic (+89/+292)	fre → / ← fadA	flavin reductase/3‑ketoacyl‑CoA thiolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,027,317	0	A	G	100.0%	134.8 / NA	39	intergenic (+89/+292)	fre/fadA	flavin reductase/3‑ketoacyl‑CoA thiolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/25);  total (14/25)

TGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCC  >  NC_000913/4027183‑4027452                                                                                                                                       |                                                                                                                                        tGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAGCCATg                                                                                                                                    <  2:912650/140‑1 (MQ=255)    gcgGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAGCCATGccc                                                                                                                                 >  1:394383/1‑140 (MQ=255)     cgGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGCAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAGCCATGCCCg                                                                                                                                >  1:1068567/1‑140 (MQ=255)             tCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAGCCATGCCCGATGCGACg                                                                                                                        >  2:1028650/1‑140 (MQ=255)             tCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAGCCATGCCCGATGCGACg                                                                                                                        <  2:1011354/140‑1 (MQ=255)              cGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAGCCATGCCCGATGCGACGc                                                                                                                       >  1:737432/1‑140 (MQ=255)                ccTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAGCCATGCCCGATGCGACGCTg                                                                                                                     <  2:737432/140‑1 (MQ=255)                  tGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAGCCATGCCCGATGCGACGCTGtt                                                                                                                   <  1:395739/140‑2 (MQ=255)                      tGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAGCCATGCCCGATGCGACGCTGTCGCGt                                                                                                               >  1:15560/1‑140 (MQ=255)                       ggCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAGCCATGCCCGATGCGACGCTGTCGCGTc                                                                                                              >  1:1003729/1‑140 (MQ=255)                           aTGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAGCCATGCCCGATGCGACGCTGTCGCGTCTTAt                                                                                                          <  2:86998/140‑1 (MQ=255)                             gCGTTTGCATTTATCTGAGATATAAAAAACCCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGCTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAGCCATGCCCGATGCGACGCTGTCGCGTCTTATCg                                                                                                        <  2:761237/140‑1 (MQ=255)                             gCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCCGTGGCATTTAGATCTATGACGTATCTGGCAAGCCATGCCCGATGCGACGCTGTCGCGTCTTATCg                                                                                                        <  1:956427/140‑1 (MQ=255)                             gCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAGCCATGCCCGATGCGACGCTGTCGCGTCTTATCg                                                                                                        <  1:206695/140‑1 (MQ=255)                             gCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAGCCATGCCCGATGCGACGCTGTCGCGTCTTATCg                                                                                                        <  1:641562/140‑1 (MQ=255)                                      tttATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAGCCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACaa                                                                                               <  1:632720/140‑1 (MQ=255)                                        tATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACCAAAATGTTAATCAGTGGCATTTAGATCTATGACGTATCTGGCAAGCCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAAt                                                                                             >  2:1891/1‑140 (MQ=255)                                                  aaaaaaaaaCCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAGCCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAAcc                                                                                   <  1:623699/138‑1 (MQ=255)                                                          aCCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAGCCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCg                                                                           >  2:611691/1‑140 (MQ=255)                                                               ccccTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAGCCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATaa                                                                      >  2:523431/1‑140 (MQ=255)                                                                              aagaaCGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCa                                                       >  2:851239/1‑140 (MQ=255)                                                                                 aaCGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAGCCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATcc                                                    <  2:584453/140‑1 (MQ=255)                                                                                   cGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAGCCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg                                                  >  2:1069695/1‑140 (MQ=255)                                                                                   cGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAGCCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg                                                  >  2:457833/1‑140 (MQ=255)                                                                                      cAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAGCCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCaa                                               <  2:15560/140‑1 (MQ=255)                                                                                       aaCTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAGCCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAt                                              <  1:1028650/140‑1 (MQ=255)                                                                                              cTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAGCCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCa                                       <  1:984279/140‑1 (MQ=255)                                                                                                gTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAGCCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCatg                                     >  1:180328/1‑140 (MQ=255)                                                                                                gTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAGCCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCatg                                     <  1:611691/140‑1 (MQ=255)                                                                                                      cAGTGGCATTTAGATCTATGACGTATCTGGCAAGCCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCt                               >  1:1074272/1‑140 (MQ=255)                                                                                                           gCATTTAGATCTATGACGTATCTGGCAAGCCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGcc                                <  2:413216/134‑1 (MQ=255)                                                                                                           gCATTTAGATCTATGACGTATCTGGCAAGCCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGcc                                >  1:413216/1‑134 (MQ=255)                                                                                                           gCATTTAGATCTATGACGTATCTGGCAAGCCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGc                          >  1:495127/1‑140 (MQ=255)                                                                                                              tttAGATCTATGACGTATCTGGCAAGCCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGAc                       >  1:215431/1‑140 (MQ=255)                                                                                                               ttAGATCTATGACGTATCTGGCAAGCCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACg                      <  2:220329/140‑1 (MQ=255)                                                                                                                         gACGTATCTGGCAAGCCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTc            <  1:1891/140‑1 (MQ=255)                                                                                                                             tATCTGGCAAGCCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTAt        <  1:457833/140‑1 (MQ=255)                                                                                                                               tCTGGCAAGCCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCg      <  2:394383/140‑1 (MQ=255)                                                                                                                               tCTGGCAAGCCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCg      <  1:803839/140‑1 (MQ=255)                                                                                                                               tCTGGCAAGCCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCg      <  1:523431/140‑1 (MQ=255)                                                                                                                               tCTGGCAAGCCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCg      <  1:474016/140‑1 (MQ=255)                                                                                                                                   gCAAGCCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGcc  <  1:1069695/140‑1 (MQ=255)                                                                                                                                    cAAGCCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATc       <  2:864418/134‑1 (MQ=38)                                                                                                                                       |                                                                                                                                        TGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCC  >  NC_000913/4027183‑4027452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑