Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,050,693	T→C	T25A (ACC→GCC)	yihA ←	GTP‑binding protein YihA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,050,693	0	T	C	100.0%	295.0 / NA	81	T25A (ACC→GCC)	yihA	GTP‑binding protein YihA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/41);  total (40/41)

AACAGGTTGATAAGCTGGGTGCGCCCTGGGGTTTTTGAGGTACGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGTATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGGTCGTCTCCGTCAGTCAAAGCTTTGTGGCGATTATACCTGAACCATAATAAAAGGCGG  >  NC_000913/4050563‑4050827                                                                                                                                   |                                                                                                                                       aaCAGGTTGATAAGCTGGGTGCGCCCTGGGGTTTTTGAGGTACGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAagg                                                                                                                               >  1:324386/1‑140 (MQ=255) aaCAGGTTGATAAGCTGGGTGCGCCCTGGGGTTTTTGAGGTACGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAagg                                                                                                                               >  1:314093/1‑140 (MQ=255) aaCAGGTTGATAAGCTGGGTGCGCCCTGGGGTTTTTGAGGTACGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAagg                                                                                                                               >  2:298120/1‑140 (MQ=255)    aGGTTGATAAGCTGGGTGCGCCCTGGGGTTTTTGAGGTACGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAaggtag                                                                                                                            <  1:1078752/140‑1 (MQ=255)    aGGTTGATAAGCTGGGTGCGCCCTGGGGTTTTTGAGGTACGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAaggtag                                                                                                                            <  2:283445/140‑1 (MQ=255)     ggTTGATAAGCTGGGTGCGCCCTGGGGTTTTTGAGGTACGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAaggtagg                                                                                                                           <  2:216794/140‑1 (MQ=255)      gTTGATAAGCTGGGTGCGCCCTGGGGTTTTTGAGGTACGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAaggtaggt                                                                                                                          >  1:319098/1‑140 (MQ=255)       ttGATAAGCTGGGTGCGCCCTGGGGTTTTTGAGGTACGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAaggtaggt                                                                                                                          <  1:648504/139‑1 (MQ=255)       ttGATAAGCTGGGTGCGCCCTGGGGTTTTTGAGGTACGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAaggtaggt                                                                                                                          >  2:648504/1‑139 (MQ=255)         gATAAGCTGGGTGCGCCCTGGGGTTTTTGAGGTACGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGc                                                                                                                       <  2:319098/140‑1 (MQ=255)          aTAAGCTGGGTGCGCCCTGGGGTTTTTGAGGTACGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCg                                                                                                                      >  2:1060942/1‑140 (MQ=255)             aGCTGGGTGCGCCCTGGGGTTTTTGAGGTACGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAat                                                                                                                   <  1:892532/140‑1 (MQ=255)              gCTGGGTGCGCCCTGGGG‑TTTTGAGGTACGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAatat                                                                                                                 <  1:262628/140‑1 (MQ=255)               cTGGGTGCGCCCTGGGGTTTTTGAGGTACGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAatat                                                                                                                 <  2:793154/140‑1 (MQ=255)                tGGGTGCGCCCTGGGGTTTTTGAGGTACGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATc                                                                                                                >  1:835581/1‑140 (MQ=255)                  ggTGCGCCCTGGGGTTTTTGAGGTACGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAg                                                                                                              >  2:441530/1‑140 (MQ=255)                    tGCGCCCTGGGGTTTTTGAGGTACGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGgc                                                                                                            >  1:548541/1‑140 (MQ=255)                     gcgcCCTGGGGTTTTTGAGGTACGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGgcg                                                                                                           <  1:801159/140‑1 (MQ=255)                     gcgcCCTGGGGTTTTTGAGGTACGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGgc                                                                                                            >  1:75263/1‑139 (MQ=255)                     gcgcCCTGGGGTTTTTGAGGTACGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGgc                                                                                                            <  2:75263/139‑1 (MQ=255)                      cgcCCTGGGGTTTTTGAGGTACGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGgcgc                                                                                                          <  1:735472/140‑1 (MQ=255)                       gcCCTGGGGTTTTTGAGGTACGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCa                                                                                                         <  2:314093/140‑1 (MQ=255)                       gcCCTGGGGTTTTTGAGGTACGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCa                                                                                                         <  2:324386/140‑1 (MQ=255)                        cccTGGGGTTTTTGAGGTACGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCAc                                                                                                        <  1:531488/140‑1 (MQ=255)                         ccTGGGGTTTTTGAGGTACGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACt                                                                                                       <  1:1060942/140‑1 (MQ=255)                         ccTGGGGGTTGTGAGGTACGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACt                                                                                                       >  2:991063/1‑140 (MQ=255)                                 ttttGAGGTACGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCAc                                                                                                        >  1:457093/1‑131 (MQ=255)                                 ttttGAGGTACGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCAc                                                                                                        <  2:457093/131‑1 (MQ=255)                                 ttttGAGGTACGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACaa                                                                                               <  2:1074215/140‑1 (MQ=255)                                          aCGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCt                                                                                      <  1:991063/140‑1 (MQ=255)                                          aCGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCt                                                                                      >  1:151373/1‑140 (MQ=255)                                               ccAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGa                                                                                 <  2:595176/140‑1 (MQ=255)                                                  ggCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATaa                                                                              >  2:1091104/1‑140 (MQ=255)                                                   gCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAGAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAAt                                                                             >  2:977663/1‑140 (MQ=255)                                                   gCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAAt                                                                             <  1:441530/140‑1 (MQ=255)                                                   gCTTTTCTGGCTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAAt                                                                             >  2:101192/1‑140 (MQ=255)                                                       ttCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCaa                                                                         <  2:323039/140‑1 (MQ=255)                                                        tCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCaaa                                                                        >  1:886055/1‑140 (MQ=255)                                                        tCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCaaa                                                                        >  1:1066641/1‑140 (MQ=255)                                                        tCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCaaa                                                                        <  2:1066641/140‑1 (MQ=255)                                                                 tCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGt                                                                   <  1:181185/136‑1 (MQ=255)                                                                 tCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGt                                                                   >  2:181185/1‑136 (MQ=255)                                                                 tCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCaaa                                                               >  2:22937/1‑140 (MQ=255)                                                                 tCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCaaa                                                               >  1:1076638/1‑140 (MQ=255)                                                                 tCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCaaa                                                               >  2:81615/1‑140 (MQ=255)                                                                 tCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCaaa                                                               >  1:509329/1‑140 (MQ=255)                                                                     cGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCgg                                                           <  1:120357/140‑1 (MQ=255)                                                                      gtgtTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCgg                                                           >  1:436572/1‑139 (MQ=255)                                                                      gtgtTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCgg                                                           <  2:436572/139‑1 (MQ=255)                                                                      gtgtTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGCGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGgt                                                          >  2:205320/1‑140 (MQ=255)                                                                       tgtTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGgtc                                                         >  2:523612/1‑140 (MQ=255)                                                                        gtTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGgtcg                                                        <  2:509329/140‑1 (MQ=255)                                                                              cgcgcTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGGTCGTCTCCg                                                  >  2:954478/1‑140 (MQ=255)                                                                              cgcgcTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGGTCGTCTCCg                                                  >  2:1079881/1‑140 (MQ=255)                                                                                  cTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGc                                                             <  2:193140/125‑1 (MQ=255)                                                                                  cTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGc                                                             >  1:193140/1‑125 (MQ=255)                                                                                     gATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGGTCGTCTCCgtcagtca                                           <  1:1079881/140‑1 (MQ=255)                                                                                               cGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGGTCGTCTCCGTCAGTCAAAGCTTtgtg                                 >  2:963355/1‑140 (MQ=255)                                                                                               cGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGGTCGTCTCCGTCAGTCAAAGCTTtgtg                                 <  2:867999/140‑1 (MQ=255)                                                                                               cGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGGTCGTCTCCGTCAGTCAAAGCTTtgtg                                 >  2:861377/1‑140 (MQ=255)                                                                                               cGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGGTCGTCTCCGTCAGTCAAAGCTTtgtg                                 <  1:22937/140‑1 (MQ=255)                                                                                               cGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGGTCGTCTCCGTCAGTCAAAGCTTtgtg                                 >  1:1043603/1‑140 (MQ=255)                                                                                               cGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGGTCGTCTCCGTCAGTCAAAGCTTtgtg                                 >  1:153945/1‑140 (MQ=255)                                                                                               cGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGGTCGTCTCCGTCAGTCAAAGCTTtgtg                                 >  1:1069704/1‑140 (MQ=255)                                                                                               cGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGGTCGTCTCCGTCAGTCAAAGCTTtgtg                                 >  1:736514/1‑140 (MQ=255)                                                                                               cGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGGTCGTCTCCGTCAGTCAAAGCTTtgtg                                 <  2:1069704/140‑1 (MQ=255)                                                                                                    gAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGGTCGTCTCCGTCAGTCAAAGCTTTGTGGCGAt                            <  2:886055/140‑1 (MQ=255)                                                                                                    gAACGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGGTCGTCTCCGTCAGTCAAAGCTTTGTGGCGAt                            <  1:1091104/140‑1 (MQ=255)                                                                                                      aCGGCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGGTCGTCTCCGTCAGTCAAAGCTTTGTGGCGATta                          <  2:736514/140‑1 (MQ=255)                                                                                                         gccagCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGGTCGTCTCCGTCAGTCAAAGCTTTGTGGCGATTATAc                       >  1:118816/5‑140 (MQ=255)                                                                                                         gCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGTGGTCGTCTCCGTCAGTCAAAGCTTTGTGGCGAt                            <  2:1094924/135‑1 (MQ=255)                                                                                                         gCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGTGGTCGTCTCCGTCAGTCAAAGCTTTGTGGCGAt                            >  1:1094924/1‑135 (MQ=255)                                                                                                         gCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGGTCGTCTCCGTCAGTCAAAGCTTTGTGGCGATTATAc                       >  2:1029682/1‑140 (MQ=255)                                                                                                         gCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGGTCGTCTCCGTCAGTCAAAGCTTTGTGGCGATTATAc                       <  1:861377/140‑1 (MQ=255)                                                                                                         gCCTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGGGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGGTCGTCTCCGTCAGTCAAAGCTTTGTGGCGATTATAc                       >  2:425138/1‑140 (MQ=255)                                                                                                         gCCAGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGGTCGTCTCCGTCAGTCAAAGCTTTGTGGCGATTATAc                       <  2:118816/140‑1 (MQ=255)                                                                                                          ccTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGGTCGTCTCCGTCAGTCAAAGCTTtgtg                                 >  2:1085607/1‑129 (MQ=255)                                                                                                          ccTGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGGTCGTCTCCGTCAGTCAAAGCTTtgtg                                 <  1:1085607/129‑1 (MQ=255)                                                                                                            tGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGGTCGTCTCCGTCAGTCAAAGCTTTGTGGCGATTATACCTg                    <  2:1076638/140‑1 (MQ=255)                                                                                                            tGCAAAAGCCACTTCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGGTCGTCTCCGTCAGTCAAAGCTTTGTGGCGATTATACCTg                    <  1:963355/140‑1 (MQ=255)                                                                                                                        ttCAATTCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGGTCGTCTCCGTCAGTCAAAGCTTTGTGGCGATTATACCTGAACCATAATaaa        >  1:1084086/1‑140 (MQ=255)                                                                                                                              tCCGGCATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGGTCGTCTCCGTCAGTCAAAGCTTTGTGGCGATTATACCTGAACCATAATAAAAGGCgg  <  2:412059/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                       AACAGGTTGATAAGCTGGGTGCGCCCTGGGGTTTTTGAGGTACGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGTATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGGTCGTCTCCGTCAGTCAAAGCTTTGTGGCGATTATACCTGAACCATAATAAAAGGCGG  >  NC_000913/4050563‑4050827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑