Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,062,890	T→C	V215A (GTG→GCG)	yihN →	putative transporter YihN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,062,890	0	T	C	100.0%	283.1 / NA	78	V215A (GTG→GCG)	yihN	putative transporter YihN
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (42/36);  total (42/36)

CATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACATTCTTCATTCCATTCCTGAAAAACATCTATCTATTGCCCGTTGCGCTGGTGGGGGCTTACG  >  NC_000913/4062756‑4063024                                                                                                                                       |                                                                                                                                       cATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGa                                                                                                                                   <  2:903587/140‑1 (MQ=255)    cTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAg                                                                                                                                <  2:179639/140‑1 (MQ=255)        ttCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACaa                                                                                                                            <  2:321731/140‑1 (MQ=255)              tCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTAt                                                                                                                      <  2:491717/140‑1 (MQ=255)                 cTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTg                                                                                                                   >  2:824175/1‑140 (MQ=255)                 cTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTg                                                                                                                   >  2:134684/1‑140 (MQ=255)                   tATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGc                                                                                                                 <  1:1038285/140‑1 (MQ=255)                       gtgATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTAt                                                                                                             <  1:1050867/140‑1 (MQ=255)                          aTTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGc                                                                                                          >  1:901929/1‑140 (MQ=255)                          aTTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGc                                                                                                          >  2:648170/1‑140 (MQ=255)                          aTTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGc                                                                                                          >  1:282104/1‑140 (MQ=255)                               cGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTa                                                                                                     >  2:913699/1‑140 (MQ=255)                                  aGGCATTATTATTTTCTTTGTCCTGAATGACAGAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACg                                                                                                  >  2:538323/1‑140 (MQ=255)                                        tattattTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGtcttct                                                                                            >  2:300750/1‑140 (MQ=255)                                           tattTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGt                                                                                                 >  2:217668/1‑132 (MQ=255)                                           tattTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGt                                                                                                 <  1:217668/132‑1 (MQ=255)                                             ttttCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGtt                                                                                       >  1:777256/1‑140 (MQ=255)                                               ttCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTa                                                                                     <  1:300750/140‑1 (MQ=255)                                               ttCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTa                                                                                     >  1:806193/1‑140 (MQ=255)                                                tCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTAc                                                                                    <  2:292034/140‑1 (MQ=255)                                                  tttGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTAcgc                                                                                  <  2:535618/140‑1 (MQ=255)                                                  tttGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTAcgc                                                                                  >  1:593887/1‑140 (MQ=255)                                                   ttGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTAcgcg                                                                                 <  1:134684/140‑1 (MQ=255)                                                    tGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCgg                                                                                >  2:113129/1‑140 (MQ=255)                                                     gTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGt                                                                               <  2:861376/140‑1 (MQ=255)                                                       ccTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTAc                                                                                    <  2:477474/133‑1 (MQ=255)                                                       ccTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTAc                                                                                    >  1:477474/1‑133 (MQ=255)                                                          gAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACt                                                                          <  2:777256/140‑1 (MQ=255)                                                           aaTGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACtg                                                                         >  2:827747/1‑140 (MQ=255)                                                           aaTGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACtg                                                                         >  2:900892/1‑140 (MQ=255)                                                           aaTGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACtg                                                                         >  1:71013/1‑140 (MQ=255)                                                                cAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGcc                                                                    >  1:382776/1‑140 (MQ=255)                                                                cAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGcc                                                                    >  2:395937/1‑140 (MQ=255)                                                                cAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGcc                                                                    >  1:433281/1‑140 (MQ=255)                                                                cAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGc                                                                     >  1:687892/1‑139 (MQ=255)                                                                cAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGc                                                                     <  2:687892/139‑1 (MQ=255)                                                                  aagaagAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTg                                                                  >  1:864408/1‑140 (MQ=255)                                                                   agaagaGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGa                                                                 <  1:843004/140‑1 (MQ=255)                                                                   agaagaGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGa                                                                 >  2:771359/1‑140 (MQ=255)                                                                    gaagaGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGAc                                                                <  2:593887/140‑1 (MQ=255)                                                                    gaagaGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGAc                                                                <  2:558199/140‑1 (MQ=255)                                                                     aagaGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACa                                                               <  2:382776/140‑1 (MQ=255)                                                                     aagaGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACa                                                               <  2:972953/140‑1 (MQ=255)                                                                        agGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACAttc                                                            >  2:691789/1‑140 (MQ=255)                                                                          gCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACAttctt                                                          >  2:916699/1‑140 (MQ=255)                                                                          gCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACCATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACAttctt                                                          >  1:208619/1‑140 (MQ=255)                                                                           cACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACAttcttc                                                         >  2:736622/1‑140 (MQ=255)                                                                           cACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACAttcttc                                                         >  1:223800/1‑140 (MQ=255)                                                                               gtccgtTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACATTCTTcattc                                                     >  1:449110/1‑140 (MQ=255)                                                                                    ttGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACATTCTTcattccattc                                                >  2:872848/1‑140 (MQ=255)                                                                                    ttGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACATTCTTcattccattc                                                >  1:832039/1‑140 (MQ=255)                                                                                         gTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACATTCTTCATTCCATTCCTGaa                                           >  2:530941/1‑140 (MQ=255)                                                                                          tGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACATTCTTcattccat                                                  >  1:450621/1‑132 (MQ=255)                                                                                          tGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACATTCTTcattccat                                                  <  2:450621/132‑1 (MQ=255)                                                                                          tGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACATTCTTCATTCCATTCCTGaaa                                          >  2:59811/1‑140 (MQ=255)                                                                                          tGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACATTCTTCATTCCATTCCTGaaa                                          <  2:433281/140‑1 (MQ=255)                                                                                            aaaaaaGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACATTCTTCATTCCATTCCTGaaaaa                                        <  1:59811/140‑1 (MQ=255)                                                                                            aaaaaaGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACATTCTTCATTCCATTCCTGaaaaa                                        <  2:832039/140‑1 (MQ=255)                                                                                               aaaGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACATTCTTCATTCCATTCCTGAAAAACat                                     <  1:113129/140‑1 (MQ=255)                                                                                                aagaagACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACATTCTTCATTCCATTCCTGAAAAACatc                                    <  1:530941/140‑1 (MQ=255)                                                                                                aagaagACGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACATTCTTCATTCCATTCCTGAAAAACatc                                    <  1:736622/140‑1 (MQ=255)                                                                                                 agaagaCGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACATTCTTCATTCCATTCCTGAAAAACatct                                   <  1:395937/140‑1 (MQ=255)                                                                                                 agaagaCGGAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACATTCTTCATTCCATTCCTGAAAAACatct                                   <  1:538323/140‑1 (MQ=255)                                                                                                        ggAGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACATTCTTCATTCCATTCCTGAAAAACATCTATCTAtt                            <  2:282104/140‑1 (MQ=255)                                                                                                          aGCATCGAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACATTCTTCATTCCATTCCTGAAAAACATCTATCTATTGc                          >  2:32474/1‑140 (MQ=255)                                                                                                                gAAAAACACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACATTCTTCATTCCATTCCTGAAAAACATCTATCTATTGCCCGTTg                    >  1:305661/1‑140 (MQ=255)                                                                                                                  aaaaCACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACATTCTTCATTCCATTCCTGAAAAACATCTATCTATTGCCCGTTgcg                  <  1:916699/140‑1 (MQ=255)                                                                                                                  aaaaCACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACATTCTTCATTCCATTCCTGAAAAACATCTATCTATTGCCCGTTgcg                  <  1:824175/140‑1 (MQ=255)                                                                                                                   aaaCACCAGTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACATTCTTCATTCCATTCCTGAAAAACATCTATCTATTGCCCGTTgcgc                 <  1:872848/140‑1 (MQ=255)                                                                                                                           gTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACATTCTTCATTCCATTCCTGAAAAACATCTATCTATTGCCCGTTGCGCtggt             <  2:149190/136‑1 (MQ=255)                                                                                                                           gTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACATTCTTCATTCCATTCCTGAAAAACATCTATCTATTGCCCGTTGCGCtggt             >  1:149190/1‑136 (MQ=255)                                                                                                                           gTATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTGTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACATTCTTCATTCCGTTCCTGAAAAACATCTATCTATTGCCCGTTGCGCTGGTgggg         >  2:751482/1‑140 (MQ=255)                                                                                                                            tATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACATTCTTCATTCCATTCCTGAAAAACATCTATCTATTGCCCGTTgcg                  <  2:593401/130‑1 (MQ=255)                                                                                                                            tATGACCTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACATTCTTCATTCCATTCCTGAAAAACATCTATCTATTGCCCGTTgcg                  >  1:593401/1‑130 (MQ=255)                                                                                                                                 ccTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACATTCTTCATTCCATTCCTGAAAAACATCTATCTATTGCCCGTTGCGCTGGTGGGGGCTTAc   >  1:541432/1‑140 (MQ=255)                                                                                                                                 ccTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACATTCTTCATTCCATTCCTGAAAAACATCTATCTATTGCCCGTTGCGCTGGTGGGGGCTTAc   >  1:103991/1‑140 (MQ=255)                                                                                                                                 ccTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACATTCTTCACTCCATTCCTGAAAAACATCTATCTATTGCCCGTTGCGCTGGTGGGGGCTTAc   >  2:275373/1‑140 (MQ=255)                                                                                                                                  cTCGGCGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACATTCTTCATTCCATTCCTGAAAAACATCTATCTATTGCCCGTTGCGCTGGTGGGGGCTTACg  <  1:900892/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       CATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGACATTCTTCATTCCATTCCTGAAAAACATCTATCTATTGCCCGTTGCGCTGGTGGGGGCTTACG  >  NC_000913/4062756‑4063024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑