Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,253,150	G→A	Q45Q (CAG→CAA)	ubiA →	4‑hydroxybenzoate octaprenyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,253,150	0	G	A	100.0%	248.7 / NA	71	Q45Q (CAG→CAA)	ubiA	4‑hydroxybenzoate octaprenyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/31);  total (40/31)

GAGTCTGACGCAGAATAAGCTGCTGGCGTTTCATCGCTTAATGCGTACGGATAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAGCTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATGGTCATGTTAAGCGCACGGCGAACCGACCACTTCCCAGCGGCGC  >  NC_000913/4253024‑4253284                                                                                                                               |                                                                                                                                       gagtCTGACGCAGAATAAGCTGCTGGCGTTTCATCGCTTAATGCGTACGGATAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTgg                                                                                                                           <  2:398503/140‑1 (MQ=255)  agtCTGACGCAGAATAAGCTGCTGGCGTTTCATCGCTTAATGCGTACGGATAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGc                                                                                                                          >  2:571439/1‑140 (MQ=255)    tCTGACGCAGAATAAGCTGCTGGCGTTTCATCGCTTAATGCGTACGGATAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCgg                                                                                                                        >  2:774112/1‑140 (MQ=255)       gACGCAGAATAAGCTGCTGGCGTTTCATTGCTTAATGCGTACGGATAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGgtgt                                                                                                                     >  2:937536/1‑140 (MQ=255)       gACGCAGAATAAGCTGCTGGCGTTTCATTGCTTAATGCGTACGGATAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGgtgt                                                                                                                     >  2:442604/1‑140 (MQ=255)         cGCAGAATAAGCTGCTGGCGTTTCATCGCTTAATGCGTACGGATAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGttt                                                                                                                   >  2:298163/1‑140 (MQ=255)           cAGAATAAGCTGCTGGCGTTTCATCGCTTAATGCGTACGGATAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGt                                                                                                                 >  2:446778/1‑140 (MQ=255)            aGAATAAGCTGCTGGCGTTTCATCGCTTAATGCGTACGGATAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTc                                                                                                                >  2:836017/1‑140 (MQ=255)            aGAATAAGCTGCTGGCGTTTCATCGCTTAATGCGTACGGATAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTc                                                                                                                >  1:911744/1‑140 (MQ=255)              aaTAAGCTGCTGGCGTTTCATCGCTTAATGCGTACGGATAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTcgc                                                                                                              >  1:678674/1‑140 (MQ=255)              aaTAAGCTGCTGGCGTTTCATCGCTTAATGCGTACGGATAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTcgc                                                                                                              >  1:62940/1‑140 (MQ=255)                   gctgctGGCGTTTCATCGCTTAATGCGTACGGATAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGgtg                                                                                                         >  2:618474/1‑140 (MQ=255)                    ctgctgGCGTTTCATCGCTTAATGCGTACGGATAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGgtgt                                                                                                        <  1:571439/140‑1 (MQ=255)                       ctgGCGTTTCATCGCTTAATGCGTACGGATAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTcgc                                                                                                              <  1:142956/131‑1 (MQ=255)                       ctgGCGTTTCATCGCTTAATGCGTACGGATAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTcgc                                                                                                              >  2:142956/1‑131 (MQ=255)                        tgGCGTTTCATCGCTTAATGCGTACGGATAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGgtg                                                                                                         >  1:130872/1‑135 (MQ=255)                        tgGCGTTTCATCGCTTAATGCGTACGGATAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGgtg                                                                                                         <  2:130872/135‑1 (MQ=255)                        tgGCGTTTCATCGCTTAATGCGTACGGATAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTgg                                                                                                    >  1:795949/1‑140 (MQ=255)                        tgGCGTTTCATCGCTTAATGCGTACGGATAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTgg                                                                                                    >  1:787949/1‑140 (MQ=255)                        tgGCGTTTCATCGCTTAATGCGTACGGATAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTgg                                                                                                    >  1:525493/1‑140 (MQ=255)                        tgGCGTTTCATCGCTTAATGCGTACGGATAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTgg                                                                                                    >  2:479426/1‑140 (MQ=255)                             tttCATCGCTTAATGCGTACGGATAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGAt                                                                                               <  1:618474/140‑1 (MQ=255)                                 aTCGCTTAATGCGTACGGATAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATgcgc                                                                                           <  2:308996/140‑1 (MQ=255)                                  ttgCTTAATGCGTACGGATAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATgcgcg                                                                                          <  1:937536/138‑1 (MQ=255)                                  ttgCTTAATGCGTACGGATAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATgcgcg                                                                                          <  1:442604/138‑1 (MQ=255)                                  tCGCTTAATGCGTACGGATAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATgcgcg                                                                                          <  1:479426/140‑1 (MQ=255)                                   cGCTTAATGCGTACGGATAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATgcgcgc                                                                                         <  2:787949/140‑1 (MQ=255)                                          tGCGTACGGATAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGa                                                                                  <  1:446778/140‑1 (MQ=255)                                            cGTACGGATAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGAtg                                                                                <  2:133628/140‑1 (MQ=255)                                                cGGATAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGa                                                                                  >  2:408685/1‑134 (MQ=255)                                                cGGATAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGa                                                                                  <  1:408685/134‑1 (MQ=255)                                                   aTAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTgtggtg                                                                         <  2:62940/140‑1 (MQ=255)                                                    tAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATgcgcgc                                                                                         <  1:351384/123‑1 (MQ=255)                                                    tAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATgcgcgc                                                                                         >  2:351384/1‑123 (MQ=255)                                                       gCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATg                                                                     >  1:721830/1‑140 (MQ=255)                                                           aTTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTa                                                                 >  1:791867/1‑140 (MQ=255)                                                            ttGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTAt                                                                >  2:181365/1‑140 (MQ=255)                                                                     tACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGc                                                       >  2:809208/1‑140 (MQ=255)                                                                             ctctGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGa                                               >  1:193461/1‑140 (MQ=255)                                                                             ctctGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGa                                               <  1:308886/140‑1 (MQ=255)                                                                               ctGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGTGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATg                                             >  2:117113/1‑140 (MQ=255)                                                                               ctGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGc                                                       >  1:54085/1‑130 (MQ=255)                                                                               ctGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGc                                                       <  2:54085/130‑1 (MQ=255)                                                                               ctGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATg                                             >  1:142417/1‑140 (MQ=255)                                                                               ctGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATg                                             <  2:279888/140‑1 (MQ=255)                                                                                tGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATgg                                            <  2:721830/140‑1 (MQ=255)                                                                                 ggCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATGGt                                           <  2:678674/140‑1 (MQ=255)                                                                                  gCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATGGTc                                          <  1:181365/140‑1 (MQ=255)                                                                                           aTGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATGGTCATGTTAAgc                                 <  2:525493/140‑1 (MQ=255)                                                                                           aTGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATGGTCATGTTAAgc                                 <  2:589975/140‑1 (MQ=255)                                                                                            tGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATGGt                                           >  2:359954/1‑129 (MQ=255)                                                                                            tGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATGGt                                           <  1:359954/129‑1 (MQ=255)                                                                                            tGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATGGTCATGTTAAgcg                                >  1:796783/1‑140 (MQ=255)                                                                                            tGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATGGTCATGTTAAgcg                                >  2:746881/1‑140 (MQ=255)                                                                                                cGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATGGTCATGTTAAGCGCACg                            <  2:65888/140‑1 (MQ=255)                                                                                                cGTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATGGTCATGTTAAGCGCACg                            <  2:796783/140‑1 (MQ=255)                                                                                                 gTTGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATGGTCATGTTAAGCGCACgg                           >  1:699356/1‑140 (MQ=255)                                                                                                  ttGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATGGTCATGTTAAGCGCACGGc                          >  1:935827/1‑140 (MQ=255)                                                                                                  ttGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATGGTCATGTTAAGCGCACGGc                          >  2:859825/1‑140 (MQ=255)                                                                                                  ttGTGGGTGGCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATGGTCATGTTAAGCGCACGGc                          >  2:192629/1‑140 (MQ=255)                                                                                                       ggtggCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATGGTCATGTTAAGCGCACGGCGAAcc                     <  1:477771/140‑1 (MQ=255)                                                                                                          ggCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATGGTCATGTTAAGCGCACGGCGAACCGAc                  <  2:935827/140‑1 (MQ=255)                                                                                                          ggCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATGGTCATGTTAAGCGCACGGCGAACCGAc                  <  2:791867/140‑1 (MQ=255)                                                                                                          ggCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATGGTCATGTTAAGCGCACGGCGAACCGAc                  <  2:795949/140‑1 (MQ=255)                                                                                                          ggCGACACCGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATGGTCATGTTAAGCGCACGGCGAACCGAc                  <  2:142417/140‑1 (MQ=255)                                                                                                            cgacctcGGGCGTTCCCCATCTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATGGTCATGTTAAGCGCACGGCGAACCGACCa                <  2:1006961/134‑1 (MQ=255)                                                                                                               cacCGGGCGTTCCCCAACTCTGGATCCTGGCGGGGTTTGTCGCGGGGGTCTGGCTGATGCGCGCTGCCGGATGTGTGGGGAATGATTATGCTGACCGCAAGGTTGATGGTCATGTTAAGCGCACGGCGAACCGACCACtt             >  1:1006961/1‑140 (MQ=255)                                                                                                                 ccGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATGGTCATGTTAAGCGCACGGCGAACCGACCACTTcc           >  2:75841/1‑140 (MQ=255)                                                                                                                 ccGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATGGTCATGTTAAGCGCACGGCGAACCGACCACTTcc           >  2:153758/1‑140 (MQ=255)                                                                                                                  cGGGCGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATGGTCATGTTAAGCGCACGGCGAACCGACCACTTccc          >  2:357217/1‑140 (MQ=255)                                                                                                                      cGTTCCCCAACTCTGGATCCTGGCGGTGTTTGTCGCGGGGGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCCGACCGCAAGTTTGATGGTCATGTTAAGCGCACGGCGAACCGACCACTTCCCAgcg      >  2:522839/1‑140 (MQ=255)                                                                                                                          ccccAACTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATGGTCATGTTAAGCGCACGGCGAACCGACCACTTCCCAGCGgcgc  >  1:625053/1‑140 (MQ=255)                                                                                                                               |                                                                                                                                       GAGTCTGACGCAGAATAAGCTGCTGGCGTTTCATCGCTTAATGCGTACGGATAAGCCAATTGGCGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAGCTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATGGTCATGTTAAGCGCACGGCGAACCGACCACTTCCCAGCGGCGC  >  NC_000913/4253024‑4253284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑