BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,270,600	A→G	E121E (GAA→GAG)	yjbQ →	UPF0047 protein YjbQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,270,600	0	A	G	99.1%	398.3 / ‑4.6	111	E121E (GAA→GAG)	yjbQ	UPF0047 protein YjbQ
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (55/55); total (56/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

Only 100 of 113 total aligned reads displayed.

ATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAGGCGCAGAACAGAGCGTGCATAATGACTGGAAAGC  >  NC_000913/4270464‑4270734

      aGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAgcatcgcatc                                                                                                                               >  1:69970/1‑140 (MQ=255)

       gCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATcc                                                                                                                              <  1:1018996/140‑1 (MQ=255)

          tGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCAcg                                                                                                                           >  1:520765/1‑140 (MQ=255)

                   gggAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATcgc                                                                                                                  >  1:267883/1‑140 (MQ=255)

                      aGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAGGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTc                                                                                                               >  1:647208/1‑140 (MQ=255)

                      aGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTc                                                                                                               >  1:1073860/1‑140 (MQ=255)

                      aGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTc                                                                                                               >  1:660142/1‑140 (MQ=255)

                      aGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTc                                                                                                               >  1:422499/1‑140 (MQ=255)

                      aGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTc                                                                                                               >  2:362751/1‑140 (MQ=255)

                      aGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTc                                                                                                               >  2:1008707/1‑140 (MQ=255)

                       gCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCg                                                                                                              >  2:804097/1‑140 (MQ=255)

                       gCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCg                                                                                                              >  2:31256/1‑140 (MQ=255)

                       gCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCg                                                                                                              >  1:390367/1‑140 (MQ=255)

                        cAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGc                                                                                                             >  1:464793/1‑140 (MQ=255)

                        cAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGc                                                                                                             >  2:794594/1‑140 (MQ=255)

                        cAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGc                                                                                                             >  2:43352/1‑140 (MQ=255)

                        cAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGc                                                                                                             >  2:354674/1‑140 (MQ=255)

                        cAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGc                                                                                                             >  1:862831/1‑140 (MQ=255)

                          gACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGcat                                                                                                           <  1:517035/140‑1 (MQ=255)

                             gATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCGCGGCGGATCGCGTCGcatcat                                                                                                        <  2:670873/140‑1 (MQ=255)

                             gATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGcatcat                                                                                                        <  2:862831/140‑1 (MQ=255)

                               tatGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAGGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATcg                                                                                                      <  2:647208/140‑1 (MQ=255)

                               tatGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATcg                                                                                                      <  2:464793/140‑1 (MQ=255)

                               tatGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATcg                                                                                                      <  2:610546/140‑1 (MQ=255)

                                  gCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGa                                                                                                   <  1:794594/140‑1 (MQ=255)

                                  gCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGa                                                                                                   <  2:977125/140‑1 (MQ=255)

                                  gCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGa                                                                                                   <  2:1073860/140‑1 (MQ=255)

                                  gCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGa                                                                                                   <  1:202826/140‑1 (MQ=255)

                                  gCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGa                                                                                                   >  1:1032571/1‑140 (MQ=255)

                                  gCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGa                                                                                                   <  1:43352/140‑1 (MQ=255)

                                  gCCTTCTCATATCAAATCCTCAATGCTGGGAACAGCGCTTGGATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCGTCCACGGCGGATCGCGTCGAATCATCGCGa                                                                                                   <  2:553045/140‑1 (MQ=255)

                                   ccTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGTATCCACGGCGGATCGCGTCGCATCATCGCGac                                                                                                  <  1:428769/140‑1 (MQ=255)

                                   ccTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGac                                                                                                  <  2:520765/140‑1 (MQ=255)

                                       ctcATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGt                                                                                                                <  1:402332/122‑1 (MQ=255)

                                       ctcATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGt                                                                                                                >  2:402332/1‑122 (MQ=255)

                                         cATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACa                                                                                            >  1:728471/1‑140 (MQ=255)

                                         cATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACa                                                                                            >  1:1001685/1‑140 (MQ=255)

                                           tatCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAg                                                                                          <  2:543375/140‑1 (MQ=255)

                                            atCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAgg                                                                                         >  2:586727/1‑140 (MQ=255)

                                                aaTCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGa                                                                                                   >  1:303996/1‑126 (MQ=255)

                                                aaTCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGa                                                                                                   <  2:303996/126‑1 (MQ=255)

                                                aaTCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAg                                                                                     >  1:620292/1‑140 (MQ=255)

                                                 aTCCTCAATGCTGGGAACATCGCTTGTATTGCC‑‑‑GCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACaa                                                                                           <  1:948371/130‑1 (MQ=255)

                                                 aTCCTCAATGCTGGGAACATCGCTTGTATTGCC‑‑‑GCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACaa                                                                                           >  2:948371/1‑130 (MQ=255)

                                                          gCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGa                                                                           <  2:728471/140‑1 (MQ=255)

                                                           cTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGAc                                                                          <  1:31256/140‑1 (MQ=255)

                                                           cTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGAc                                                                          <  2:439867/140‑1 (MQ=255)

                                                                 aCATCGCTTGTATTGCCGGTGCATAAAGGGAGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTc                                                                    <  2:669894/140‑1 (MQ=255)

                                                                   aTCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGAc                                                                          >  2:1014982/1‑132 (MQ=255)

                                                                   aTCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGAc                                                                          <  1:1014982/132‑1 (MQ=255)

                                                                   aTCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCgg                                                                  >  2:808407/1‑140 (MQ=255)

                                                                    tCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGa                                                                 <  1:132774/140‑1 (MQ=255)

                                                                    tCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGa                                                                 <  2:888506/140‑1 (MQ=255)

                                                                    tCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGa                                                                 <  1:354674/140‑1 (MQ=255)

                                                                    tCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGa                                                                 <  1:1013209/140‑1 (MQ=255)

                                                                     cGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAg                                                                >  1:561859/1‑140 (MQ=255)

                                                                     cGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAg                                                                >  1:469997/1‑140 (MQ=255)

                                                                     cGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAg                                                                >  1:742987/1‑140 (MQ=255)

                                                                      gCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGt                                                               <  2:536601/140‑1 (MQ=255)

                                                                       cTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGtt                                                              >  2:867265/1‑140 (MQ=255)

                                                                         tGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGc                                                            <  2:267883/140‑1 (MQ=255)

                                                                             ttGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACa                                                        >  2:340282/1‑140 (MQ=255)

                                                                                    tGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGc                                                 >  2:963280/1‑140 (MQ=255)

                                                                                     gCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCa                                                <  1:352515/140‑1 (MQ=255)

                                                                                     gCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCa                                                >  1:619927/1‑140 (MQ=255)

                                                                                     gCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCa                                                <  2:742834/140‑1 (MQ=255)

                                                                                     gCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCa                                                >  1:891901/1‑140 (MQ=255)

                                                                                          aaGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCa                                           <  1:340282/140‑1 (MQ=255)

                                                                                          aaGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCa                                           >  2:152119/1‑140 (MQ=255)

                                                                                               cGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAAcc                                      >  2:272631/1‑140 (MQ=255)

                                                                                               cGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAAcc                                      <  2:69970/140‑1 (MQ=255)

                                                                                                gTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCa                                     >  2:299346/1‑140 (MQ=255)

                                                                                                gTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCa                                     >  2:1268/1‑140 (MQ=255)

                                                                                                 tATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAAcc                                      >  2:206645/1‑138 (MQ=255)

                                                                                                 tATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAAcc                                      <  1:206645/138‑1 (MQ=255)

                                                                                                 tATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAg                                    <  2:561859/140‑1 (MQ=255)

                                                                                                   ttCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAGgc                                  <  2:742987/140‑1 (MQ=255)

                                                                                                    tCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAGgcg                                 <  1:963280/140‑1 (MQ=255)

                                                                                                    tCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAGgcg                                 <  2:1032571/140‑1 (MQ=255)

                                                                                                      aGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAGGCGCa                               <  1:989495/140‑1 (MQ=255)

                                                                                                        aCCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAGGCGCAGa                             >  2:667444/1‑140 (MQ=255)

                                                                                                        aCCGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAGGCGCAGa                             >  1:945082/1‑140 (MQ=255)

                                                                                                        aCCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAGGCGCAGa                             >  1:389807/1‑140 (MQ=255)

                                                                                                         ccGGCACCTGGTAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAGGCGCAGaa                            <  1:831889/140‑1 (MQ=255)

                                                                                                         ccGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTTCAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAGGCGCAGaa                            <  2:63049/140‑1 (MQ=255)

                                                                                                         ccGGCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAGGCGCAGaa                            <  2:873891/140‑1 (MQ=255)

                                                                                                            gCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAGGCGCAGAACag                         >  2:672379/1‑140 (MQ=255)

                                                                                                            gCACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAGGCGCAGAACag                         >  1:401619/1‑140 (MQ=255)

                                                                                                             cACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAGGCGCAGAACaga                        >  2:722158/1‑140 (MQ=255)

                                                                                                             cACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAGGCGCAGAACaga                        >  1:957709/1‑140 (MQ=255)

                                                                                                             cACCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAGGCGCAGAACaga                        >  2:119916/1‑140 (MQ=255)

                                                                                                              aCCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAGGCGCAGAACagag                       <  1:1268/140‑1 (MQ=255)

                                                                                                              aCCTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAGGCGCAGAACagag                       <  1:722158/140‑1 (MQ=255)

                                                                                                                cTGGCAAGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAGGCGCAGAACAGAGCg                     <  2:469997/140‑1 (MQ=255)

                                                                                                                      aGGCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAGGCGCAGAACAGAGCGTGCATa               >  2:745849/1‑140 (MQ=255)

                                                                                                                       ggCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAGGCGCAGAACAGAGCGTGCATaa              >  1:442563/1‑140 (MQ=255)

                                                                                                                       ggCATTTGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAGGCGCAGAACAGAGCGTGCATaa              >  2:344055/1‑140 (MQ=255)

                                                                                                                             tGGCTGGGGGAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAGGCGCAGAACAGAGCGTGCATAATGACTg        <  2:390367/140‑1 (MQ=255)

                                                                                                                                  gggggAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAGGCGCAGAACAGAGCGTGCATAATGACTGGAAAg   <  2:660142/140‑1 (MQ=255)

                                                                                                                                   ggggAGCATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCGTGGCAAAACCAGGCGCAGAACAGAGCGTGCATAATGACTGGAAAGc  >  1:94881/1‑140 (MQ=255)

ATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAGGCGCAGAACAGAGCGTGCATAATGACTGGAAAGC  >  NC_000913/4270464‑4270734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`