Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,287,164	A→G	Y70H (TAC→CAC)	acs ←	acetyl‑CoA synthetase (AMP‑forming)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,287,164	0	A	G	100.0%	253.7 / NA	70	Y70H (TAC→CAC)	acs	acetyl‑CoA synthetase (AMP‑forming)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/36);  total (34/36)

TGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTACCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTGGTAAGGTTTGATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAGAAGGTATCAGGTACGTTAATAGATTGTTGATACATCGCC  >  NC_000913/4287031‑4287297                                                                                                                                      |                                                                                                                                      tGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCAttt                                                                                                                                 >  1:144934/1‑140 (MQ=255) tGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGAT‑‑‑GGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAAt                                                                                                                              <  1:1017381/140‑1 (MQ=255)  gCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTa                                                                                                                                >  1:672921/1‑140 (MQ=255)  gCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTa                                                                                                                                <  2:311525/140‑1 (MQ=255)    aGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAAt                                                                                                                              <  1:868856/140‑1 (MQ=255)     gCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATg                                                                                                                             <  2:912561/140‑1 (MQ=255)       tctTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGa                                                                                                                           <  1:611068/140‑1 (MQ=255)           tataGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGAcaca                                                                                                                       >  2:589481/1‑140 (MQ=255)           tataGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGAcaca                                                                                                                       >  1:1004003/1‑140 (MQ=255)           tataGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTAGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGAcaca                                                                                                                       >  2:761964/1‑140 (MQ=255)                cTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTAcc                                                                                                                  >  2:1040566/1‑140 (MQ=255)                cTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTAcc                                                                                                                  >  2:155404/1‑140 (MQ=255)                   atatGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGTCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCggg                                                                                                               >  1:125598/1‑140 (MQ=255)                   atatGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTAcc                                                                                                                  <  2:1031233/137‑1 (MQ=255)                   atatGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTAcc                                                                                                                  >  1:1031233/1‑137 (MQ=255)                   atatGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCggg                                                                                                               >  1:72751/1‑140 (MQ=255)                   atatGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCggg                                                                                                               >  1:627510/1‑140 (MQ=255)                   atatGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCggg                                                                                                               >  1:508345/1‑140 (MQ=255)                   atatGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCggg                                                                                                               <  2:144934/140‑1 (MQ=255)                   atatGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCggg                                                                                                               >  1:102578/1‑140 (MQ=255)                     atGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCggggg                                                                                                             >  1:154036/1‑140 (MQ=255)                       gTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCa                                                                                                           >  1:830754/1‑140 (MQ=255)                          tGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTAcc                                                                                                                  <  2:237348/130‑1 (MQ=255)                          tGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTAcc                                                                                                                  >  1:237348/1‑130 (MQ=255)                             tctGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAaggag                                                                                                     <  1:511551/140‑1 (MQ=255)                             tctGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAaggag                                                                                                     >  1:303481/1‑140 (MQ=255)                              ctggctggCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAaggagg                                                                                                    <  1:589481/140‑1 (MQ=255)                               tggctggcGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGgt                                                                                                   >  1:1014520/1‑140 (MQ=255)                               tggctggcGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGgt                                                                                                   <  2:39413/140‑1 (MQ=255)                                       gtcgtcGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCa                                                                                           <  2:1014520/140‑1 (MQ=255)                                          gtcgCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCt                                                                                        <  1:303827/140‑1 (MQ=255)                                           tcgCCTTCTCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCACCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCtt                                                                                       >  2:1078253/1‑140 (MQ=255)                                           tcgCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTc                                                                                            >  2:580956/1‑135 (MQ=255)                                           tcgCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTc                                                                                            <  1:580956/135‑1 (MQ=255)                                           tcgCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCAcc                                                                                         <  1:106319/138‑1 (MQ=255)                                           tcgCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCAcc                                                                                         >  2:106319/1‑138 (MQ=255)                                           tcgCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCtt                                                                                       <  2:672921/140‑1 (MQ=255)                                            cgCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAAATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTc                                                                                      <  1:60828/140‑1 (MQ=255)                                                ttCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTGGt                                                                                  <  1:1040566/140‑1 (MQ=255)                                                ttCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTGGt                                                                                  <  2:830754/140‑1 (MQ=255)                                                  cccAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTGGTaa                                                                                >  1:655825/1‑140 (MQ=255)                                                             gCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTgg                                                                                   <  1:551606/126‑1 (MQ=255)                                                             gCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTgg                                                                                   >  2:551606/1‑126 (MQ=255)                                                                          cGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTGGTAAGGTTTGATCCAGTCAAGAAttttt                                                        >  2:160731/1‑140 (MQ=255)                                                                                 ttGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATTGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTGGTAAGGTTTGATCCAGTCAAGAATTTTTCCCTGtt                                                 <  2:46852/140‑1 (MQ=255)                                                                                    cAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTGGTAAGGTTTGATCCAGTCAAGAATTTTTCCCTGTTCGc                                              >  1:331630/1‑140 (MQ=255)                                                                                      gATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTGGTAAGGTTTGATCCAGTCAAGAATTTTTCCCTGTTCGccc                                            <  1:761964/140‑1 (MQ=255)                                                                                      gATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTGGTAAGGTTTGATCCAGTCAAGAATTTTTCCCTGTTCGccc                                            <  2:250953/140‑1 (MQ=255)                                                                                         ggCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTGGTAAGGTTTGATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAg                                         >  2:846133/1‑140 (MQ=255)                                                                                         ggCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTGGTAAGGTTTGATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAg                                         >  2:433268/1‑140 (MQ=255)                                                                                           cGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTGGTAAGGTTTGATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAGaa                                       <  2:923812/140‑1 (MQ=255)                                                                                              tCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTGGTAAGGTTTGATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAGAAGGt                                    >  1:334985/1‑140 (MQ=255)                                                                                                aaGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTGGTAAGGTTTGATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAGAAGGTAt                                  <  2:1004003/140‑1 (MQ=255)                                                                                                aaGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTGGTAAGGTTTGATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAGAAGGTAt                                  <  2:575965/140‑1 (MQ=255)                                                                                                aaGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTGGTAAGGTTTGATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAGAAGGTAt                                  >  2:427245/1‑140 (MQ=255)                                                                                                 aGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTGGTAAGGTTTGATCCAGTCAAGAATTTTTCCCTGtt                                                 >  2:946886/1‑124 (MQ=255)                                                                                                 aGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTGGTAAGGTTTGATCCAGTCAAGAATTTTTCCCTGtt                                                 <  1:946886/124‑1 (MQ=255)                                                                                                  ggCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTGGTAAGGTTTGATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAGAAGGTATCa                                <  2:331630/140‑1 (MQ=255)                                                                                                       tttGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTGGTAAGGTTTGATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAGAAGGTATCAGGTAc                           >  2:1049775/1‑140 (MQ=255)                                                                                                        ttGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTGGTAAGGTTTGATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAGAAGGTATCAGGTACg                          <  1:1049775/140‑1 (MQ=255)                                                                                                         tGCCGCCAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGAGTTTTTCACCTTCTGGTAAGGTTTGATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAGAAGGTATCAGGTACGt                         <  2:735403/140‑1 (MQ=255)                                                                                                           ccgccAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTGGTAAGGTTTGATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAGAAGGTATCAGGTACGTTa                       <  2:334985/140‑1 (MQ=255)                                                                                                             gccAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTGGTAAGGTTTGATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAGAAGGTATCAGGTACGTTAAt                     <  2:125598/140‑1 (MQ=255)                                                                                                             gccAGATTCAGCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTGGTAAGGTTTGATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAGAAGGTATCAGGTACGTTAAt                     <  1:160731/140‑1 (MQ=255)                                                                                                                       gCGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTGGTAAGGTTTGATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAGAAGGTATCAGGTACGTTAATAGATTGTTGa           <  1:1078253/140‑1 (MQ=255)                                                                                                                        cGTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTGGTAAGGTTTGATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAGAAGGTATCAGGTACGTTAATAGATTGTTGAt          >  2:852128/1‑140 (MQ=255)                                                                                                                         gTGCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTGGTAAGGTTTGATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAGAAGGTATCAGGTACGTTAATAGATTGTTGATa         <  1:427245/140‑1 (MQ=255)                                                                                                                           gCCGTCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTGGTAAGGTTTGATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAGAAGGTATCAGGTACGTTAATAGATTGTTGATACa       <  2:154036/140‑1 (MQ=255)                                                                                                                               tCCTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTGGTAAGGTTTGATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAGAAGGTATCAGGTACGTTAATAGATTGTTGATACATCGc   >  1:114897/1‑140 (MQ=255)                                                                                                                                ccTCGTGCCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTGGTAAGGTTTGATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAGAAGGTATCAGGTACGTTAATAGATTGTTGATACATCGcc  >  1:802437/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                      TGCAGCTCTTTATAGCTGATATGTTTGCTCTGGCTGGCGTCGTCGCCTTCCCAGATGATGGCGGTACGATCGCCGTTTTCTTGCAGATGGCGGTCAAGGCAGTTTGCCGCCAGATTCAGCGTGCCGTCCTCGTACCATTTAATGGACACATTACCGGGGGCAAAGGAGGTGTTTTTCACCTTCTGGTAAGGTTTGATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAGAAGGTATCAGGTACGTTAATAGATTGTTGATACATCGCC  >  NC_000913/4287031‑4287297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑