Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,434,440	A→G	I110V (ATT→GTT)	ytfH →	putative transcriptional regulator YtfH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,434,440	0	A	G	100.0%	247.3 / NA	68	I110V (ATT→GTT)	ytfH	putative transcriptional regulator YtfH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/35);  total (33/35)

TTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCATGGCTTGTAGGGCGGATAAGATGCGCCAGCATCGCATC  >  NC_000913/4434311‑4434568                                                                                                                                  |                                                                                                                                 ttACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGaa                                                                                                                        >  2:474183/1‑140 (MQ=255)        cGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAAtt                                                                                                                      <  2:253166/135‑1 (MQ=255)        cGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAAtt                                                                                                                      >  1:253166/1‑135 (MQ=255)        cGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAAt                                                                                                                       >  1:768722/1‑134 (MQ=255)        cGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAAt                                                                                                                       <  2:768722/134‑1 (MQ=255)        cGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGccc                                                                                                                 <  1:647919/140‑1 (MQ=255)        cGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGccc                                                                                                                 >  1:751263/1‑140 (MQ=255)        cGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGccc                                                                                                                 >  2:647919/1‑140 (MQ=255)          ttAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGa                                                                                                               >  2:50625/1‑140 (MQ=255)          ttAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGa                                                                                                               >  2:89537/1‑140 (MQ=255)            aGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAgg                                                                                                             >  2:552426/1‑140 (MQ=255)                   gATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGc                                                                                                      >  2:750613/1‑140 (MQ=255)                   gATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGc                                                                                                      <  1:474183/140‑1 (MQ=255)                       gTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTg                                                                                                  <  1:552426/140‑1 (MQ=255)                       gTTTTCTTAACCGTATCGCGTATCCGGGGGTGCCGCCGCATGTGGAATATAGCCTCACCCCGCTGGGCGAGCAGGGGAGGGAAAGGGTTGCTGCCCTGGCGGACTGGGTTGGGTTGAATTTGCCCGGGGGGTTGGCgggg                                                                                                  >  2:177054/1‑138 (MQ=255)                        ttttCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGc                                                                                                      <  1:1033045/135‑1 (MQ=255)                        ttttCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGc                                                                                                      >  2:1033045/1‑135 (MQ=255)                            tttAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGa                                                                                             <  1:177054/139‑1 (MQ=255)                             ttAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGAt                                                                                            >  1:26166/1‑140 (MQ=255)                               aaCCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGa                                                                                          <  1:594631/140‑1 (MQ=255)                                  cGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACg                                                                                       <  1:976662/140‑1 (MQ=255)                                    tATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTg                                                                                     >  1:746516/1‑140 (MQ=255)                                    tATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTg                                                                                     >  2:570340/1‑140 (MQ=255)                                    tATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTg                                                                                     <  1:50625/140‑1 (MQ=255)                                      tCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCg                                                                                   >  2:276218/1‑140 (MQ=255)                                      tCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCg                                                                                   >  2:640087/1‑140 (MQ=255)                                         cgTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCa                                                                                <  2:47751/140‑1 (MQ=255)                                           tATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGt                                                                                      >  2:487987/1‑132 (MQ=255)                                           tATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGt                                                                                      <  1:487987/132‑1 (MQ=255)                                              ccGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCa                                                                                <  2:229857/135‑1 (MQ=255)                                              ccGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCa                                                                                >  1:229857/1‑135 (MQ=255)                                                  tggtgCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGc                                                                         <  2:119942/138‑1 (MQ=255)                                                  tggtgCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGc                                                                         >  1:119942/1‑138 (MQ=255)                                                    gtgCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGa                                                                     <  1:89537/140‑1 (MQ=255)                                                     tgCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGTGGTGCGGGATGAACGTGCGGCATAACTGCCgg                                                                      >  1:983077/1‑138 (MQ=255)                                                     tgCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGTGGTGCGGGATGAACGTGCGGCATAACTGCCgg                                                                      <  2:983077/138‑1 (MQ=255)                                                        cgccgcATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGa                                                                     >  2:948992/1‑136 (MQ=255)                                                        cgccgcATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGa                                                                     <  1:948992/136‑1 (MQ=255)                                                        cgccgcATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATgcg                                                                 <  1:276218/140‑1 (MQ=255)                                                              aTGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGc                                                           <  1:640087/140‑1 (MQ=255)                                                                 tgGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCtt                                                         <  2:882558/139‑1 (MQ=255)                                                                 tgGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCtt                                                         >  1:254298/1‑139 (MQ=255)                                                                 tgGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCtt                                                         >  1:882558/1‑139 (MQ=255)                                                                 tgGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCtt                                                         <  2:254298/139‑1 (MQ=255)                                                                  ggAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTAt                                                       <  2:751263/140‑1 (MQ=255)                                                                     atatAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTg                                                    <  1:750613/140‑1 (MQ=255)                                                                       ataGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGt                                                  <  1:627850/140‑1 (MQ=255)                                                                        taGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGGGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGGGCTGGGCTTATTTGGtt                                                 >  2:627850/1‑139 (MQ=255)                                                                         aGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCt                                                >  1:82183/1‑140 (MQ=255)                                                                          gCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAAGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTa                                               <  2:255922/140‑1 (MQ=255)                                                                           ccTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTAt                                                       >  2:409801/1‑131 (MQ=255)                                                                           ccTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTAt                                                       <  1:409801/131‑1 (MQ=255)                                                                           ccTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTAt                                                       <  1:591422/131‑1 (MQ=255)                                                                           ccTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTAt                                                       >  2:591422/1‑131 (MQ=255)                                                                           ccTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTAc                                              >  2:112068/1‑140 (MQ=255)                                                                              cACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGc                                                           <  1:909809/124‑1 (MQ=255)                                                                              cACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGc                                                           >  2:909809/1‑124 (MQ=255)                                                                                cgccgcTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGc                                         <  1:570340/140‑1 (MQ=255)                                                                                cgccgcTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGc                                         >  1:483037/1‑140 (MQ=255)                                                                                cgccgcTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGc                                         <  2:918277/140‑1 (MQ=255)                                                                                   cgcTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGc                                         <  2:1094356/137‑1 (MQ=255)                                                                                   cgcTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGc                                         >  1:1094356/1‑137 (MQ=255)                                                                                        ggCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCATGGCTTg                                 <  2:26166/140‑1 (MQ=255)                                                                                           gAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCATGGCTTGTAg                              <  2:746516/140‑1 (MQ=255)                                                                                            aGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCATGGCTTGTAgg                             <  2:483037/140‑1 (MQ=255)                                                                                                      cGAAAAGGTTGCTGCACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCATGGCTTGTAGGGCGGATAAGa                   <  1:112068/140‑1 (MQ=255)                                                                                                                   gcACTGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCATGGCTTGTAGGGCGGATAAGATGCGCCAgcatcg      >  2:192519/1‑140 (MQ=255)                                                                                                                       tGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCATGGCTTGTAGGGCGGATAAGATGCGCCAgcatcgcatc  >  1:680814/1‑140 (MQ=255)                                                                                                                       tGGCGGACTGGGTTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCATGGCTTGTAGGGCGGATAAGATGCGCCAgcatcgcatc  >  1:881040/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                 TTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCATGGCTTGTAGGGCGGATAAGATGCGCCAGCATCGCATC  >  NC_000913/4434311‑4434568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑