Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,450,726	A→G	K255K (AAA→AAG)	ytfQ →	galactofuranose ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,450,726	0	A	G	100.0%	254.3 / NA	70	K255K (AAA→AAG)	ytfQ	galactofuranose ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/34);  total (36/34)

TGGTTTACGCCCATAACGACGACATGGTGATTGGTGCAATTCAGGCAATTAAAGAAGCGGGCCTGAAACCGGGCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAAGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCC  >  NC_000913/4450605‑4450859                                                                                                                          |                                                                                                                                      tGGTTTACGCCCATAACGACGACATGGTGATTGGTGCAATTCAGGCAATTAAAGAAGCGGGCCTGAAACCGGGCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGgcgaa                                                                                                                     <  2:309891/140‑1 (MQ=255)              tAACGACGACATGGTGATTGGTGCAATTCAGGCAATTAAAGAAGCGGGCCTGAAACCGGGCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGc                                                                                                             <  2:933222/135‑1 (MQ=255)              tAACGACGACATGGTGATTGGTGCAATTCAGGCAATTAAAGAAGCGGGCCTGAAACCGGGCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGc                                                                                                             >  1:933222/1‑135 (MQ=255)              tAACGACGACATGGTGATTGGTGCAATTCAGGCAATTAAAGAAGCGGGCCTGAAACCGGGCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAgtg                                                                                                        >  2:332799/1‑140 (MQ=255)              tAACGACGACATGGTGATTGGTGCAATTCAGGCAATTAAAGAAGCGGGCCTGAAACCGGGCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAgtg                                                                                                        >  2:196820/1‑140 (MQ=255)              tAACGACGACATGGTGATTGGTGCAATTCAGGCAATTAAAGAAGCGGGCCTGAAACCGGGCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAgtg                                                                                                        >  2:132827/1‑140 (MQ=255)              tAACGACGACATGGTGATTGGTGCAATTCAGGCAATTAAAGAAGCGGGCCTGAAACCGGGCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAgtg                                                                                                        >  2:837800/1‑140 (MQ=255)               aaCGACGACATGGTGATTGGTGCAATTCAGGCAATTAAAGAAGCGGGCCTGAAACCGGGCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAgtgt                                                                                                       <  2:617251/140‑1 (MQ=255)                    cgacATGGTGATTGGTGCAATTCAGGCAATTAAAGAAGCGGGCCTGAAACCGGGCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAAc                                                                                                  <  2:222055/140‑1 (MQ=255)                         tGGTGATTGGTGCAATTCAGGCAATTAAAGAAGCGGGCCTGAAACCGGGCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACg                                                                                             <  1:837800/140‑1 (MQ=255)                           gTGATTGGTGCAATTCAGGCAATTAAAGAAGCGGGCCTGAAACCGGGCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGcc                                                                                           <  2:422043/140‑1 (MQ=255)                            tGATTGGTGCAATTCAGGCAATTAAAGAAGCGGGCCTGAAACCGGGCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCg                                                                                          >  1:210108/1‑140 (MQ=255)                                 ggTGCAATTCAGGCAATTAAAGAAGCGGGCCTGAAACCGGGCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAatat                                                                                     >  1:850659/1‑140 (MQ=255)                                  gTGCAATTCAGGCAATTAAAGAAGCGGGCCTGAAACCGGGCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATg                                                                                    >  2:708635/1‑140 (MQ=255)                                  gTGCAATTCAGGCAATTAAAGAAGCGGGCCTGAAACCGGGCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATg                                                                                    >  1:569701/1‑140 (MQ=255)                                   tGCAATTCAGGCAATTAAAGAAGCGGGCCTGAAACCGGGCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATgg                                                                                   >  2:662453/1‑140 (MQ=255)                                    gCAATTCAGGCAATTAAAGAAGCGGGCCTGAAACCGGGCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACg                                                                                             >  2:259860/1‑129 (MQ=255)                                    gCAATTCAGGCAATTAAAGAAGCGGGCCTGAAACCGGGCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACg                                                                                             <  1:259860/129‑1 (MQ=255)                                     cAATTCAGGCAATTAAAGAAGCGGGCCTGAAACCGGGCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCa                                                                                 >  1:691800/1‑140 (MQ=255)                                             gCAATTAAAGAAGCGGGCCTGAAACCGGGCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGc                                                                         >  2:665062/1‑140 (MQ=255)                                                  tAAAGAAGCGGGCCTGAAACCGGGCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCg                                                                    >  1:284535/1‑140 (MQ=255)                                                  tAAAGAAGCGGGCCTGAAACCGGGCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCg                                                                    <  1:1543/140‑1 (MQ=255)                                                     agaagCGGGCCTGAAACCGGGCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGAcg                                                                 <  2:672900/140‑1 (MQ=255)                                                     agaagCGGGCCTGAAACCGGGCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGAcg                                                                 <  2:569701/140‑1 (MQ=255)                                                     agaagCGGGCCTGAAACCGGGCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGAcg                                                                 <  2:16699/140‑1 (MQ=255)                                                        agCGGGCCTGAAACCGGGCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACgcgc                                                              <  1:471007/140‑1 (MQ=255)                                                         gCGGGCCTGAAACCGGGCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGGCCCGCCCTCGgcgcgcc                                                             >  2:888617/1‑139 (MQ=255)                                                          cGGGCCTGAAACCGGGCAAAGATATCCTCACGGGTTCCATCGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACgcgc                                                              >  2:656297/1‑138 (MQ=255)                                                          cGGGCCTGAAACCGGGCAAAGATATCCTCACGGGTTCCATCGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACgcgc                                                              <  1:656297/138‑1 (MQ=255)                                                           gggCCTGAAACCGGGCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGAcg                                                                 >  1:482897/1‑134 (MQ=255)                                                           gggCCTGAAACCGGGCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGAcg                                                                 <  2:482897/134‑1 (MQ=255)                                                           gggCCTGAAACCGGGCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTgg                                                           >  2:907970/1‑140 (MQ=255)                                                            ggCCTGAAACCGGGCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGGCCCGCCCTCGACGCGCTggg                                                          >  2:703064/1‑139 (MQ=255)                                                               cTGAAACCGGGCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAGTATGGCAGGTCCCGCCTTCGACGCGCTGGAGaa                                                       <  1:547115/140‑1 (MQ=255)                                                                       gggCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGAcgccc                                                              >  1:792137/1‑123 (MQ=255)                                                                       gggCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACgcgc                                                              <  2:792137/125‑1 (MQ=255)                                                                       gggCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACaaaa                                               >  1:656474/1‑140 (MQ=255)                                                                        ggCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGgag                                                         <  2:494057/129‑1 (MQ=255)                                                                        ggCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGgag                                                         >  1:494057/1‑129 (MQ=255)                                                                        ggCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACaaaaa                                              >  1:1048595/1‑140 (MQ=255)                                                                           aaaGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGa                                           <  1:626524/140‑1 (MQ=255)                                                                              gATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACgg                                        <  1:888617/140‑1 (MQ=255)                                                                                 atCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCAc                                     <  2:143147/140‑1 (MQ=255)                                                                                 atCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCAc                                     <  1:6819/140‑1 (MQ=255)                                                                                       aCGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGcc                               >  2:767012/1‑140 (MQ=255)                                                                                        cGGGTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCt                              <  1:907970/140‑1 (MQ=255)                                                                                         gggTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTg                             >  2:494229/1‑140 (MQ=255)                                                                                           gTTCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGaa                           <  1:494229/140‑1 (MQ=255)                                                                                            ttCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCAc                                     <  1:495489/129‑1 (MQ=255)                                                                                            ttCCATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAAAACAAAAAAGACGGCAc                                     >  2:495489/1‑129 (MQ=255)                                                                                               cATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGc                       >  1:334162/1‑140 (MQ=255)                                                                                               cATTGACGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGc                       <  1:767012/140‑1 (MQ=255)                                                                                                    aCGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACg                  <  1:420106/140‑1 (MQ=255)                                                                                                     cGGTGTACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGt                 >  1:1047655/1‑140 (MQ=255)                                                                                                       gtgtACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTa               >  1:225062/1‑140 (MQ=255)                                                                                                        tgtACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCATCATGCCTGAAAAGCTGACGTTaa              >  1:484900/1‑140 (MQ=255)                                                                                                        tgtACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTaa              <  2:334162/140‑1 (MQ=255)                                                                                                         gtACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAAc             <  1:487604/140‑1 (MQ=255)                                                                                                          tACCGGACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAAcc            >  2:685978/1‑140 (MQ=255)                                                                                                               gACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTGCCGCCCTCCGCCCGCTGGGGAAAAAAAAAAAAAACGGCACCACGCCTGAAAAGCTGAAGTTAACCAAATc       >  2:487604/1‑140 (MQ=255)                                                                                                               gACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATc       <  2:656474/140‑1 (MQ=255)                                                                                                               gACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATc       <  1:665062/140‑1 (MQ=255)                                                                                                               gACATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATc       <  1:132827/140‑1 (MQ=255)                                                                                                               agcATCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATc       <  1:100054/138‑1 (MQ=255)                                                                                                                  aTCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATc       >  2:599503/1‑137 (MQ=255)                                                                                                                  aTCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATc       <  1:599503/137‑1 (MQ=255)                                                                                                                   tCTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCAcc   <  1:327187/140‑1 (MQ=255)                                                                                                                    cTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCAccc  >  1:577260/1‑140 (MQ=255)                                                                                                                    cTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCAccc  >  1:409159/1‑140 (MQ=255)                                                                                                                    cTACAAGGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCAccc  >  1:928937/1‑140 (MQ=255)                                                                                                                          |                                                                                                                                      TGGTTTACGCCCATAACGACGACATGGTGATTGGTGCAATTCAGGCAATTAAAGAAGCGGGCCTGAAACCGGGCAAAGATATCCTCACGGGTTCCATTGACGGTGTACCGGACATCTACAAAGCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACCC  >  NC_000913/4450605‑4450859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑