Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,468,925	C→T	A434V (GCC→GTC)	mgtA →	Mg(2(+)) importing P‑type ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,468,925	0	C	T	100.0%	262.1 / NA	73	A434V (GCC→GTC)	mgtA	Mg(2(+)) importing P‑type ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (45/28);  total (45/28)

CCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCG  >  NC_000913/4468790‑4469061                                                                                                                                        |                                                                                                                                         ccGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCATACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGt                                                                                                                                      >  1:47572/1‑140 (MQ=255) ccGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGt                                                                                                                                      >  1:785462/1‑140 (MQ=255)       tctcCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTgg                                                                                                                                >  2:670731/1‑140 (MQ=255)         tcCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCa                                                                                                                              <  2:786070/140‑1 (MQ=255)         tcCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCa                                                                                                                              <  1:529053/140‑1 (MQ=255)         tcCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCa                                                                                                                              <  1:34805/140‑1 (MQ=255)          ccGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCaa                                                                                                                             >  2:667309/1‑140 (MQ=255)            ggTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCaaaa                                                                                                                           >  2:460846/1‑140 (MQ=255)               aaaaCCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAAt                                                                                                                        <  2:785462/140‑1 (MQ=255)                  aCCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATtga                                                                                                                     <  1:594433/140‑1 (MQ=255)                   ccAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATtgat                                                                                                                    <  1:670731/140‑1 (MQ=255)                       cGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATgaga                                                                                                                <  1:1047543/140‑1 (MQ=255)                       cGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATgaga                                                                                                                >  2:1047543/1‑140 (MQ=255)                               tgctgcATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGttt                                                                                                        <  1:463296/140‑1 (MQ=255)                                         cgcgTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGc                                                                                              >  1:856032/1‑140 (MQ=255)                                         cgcgTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGc                                                                                              >  2:799354/1‑140 (MQ=255)                                          gcgTGGTTGAACAGTCATTATCATACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCg                                                                                             <  2:47572/140‑1 (MQ=255)                                                   aaCAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGAt                                                                                    <  1:310752/140‑1 (MQ=255)                                                   aaCAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGAt                                                                                    >  1:736224/1‑140 (MQ=255)                                                    aCAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATg                                                                                   <  2:856032/140‑1 (MQ=255)                                                          aTTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGt                                                                                  >  1:517714/1‑135 (MQ=255)                                                          aTTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGt                                                                                  <  2:517714/135‑1 (MQ=255)                                                              tCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAg                                                                         <  2:115370/140‑1 (MQ=255)                                                                 gACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTgg                                                                      >  1:267358/1‑140 (MQ=255)                                                                  aCCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGc                                                                     >  2:750121/1‑140 (MQ=255)                                                                  aCCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGc                                                                     >  2:722667/1‑140 (MQ=255)                                                                  aCCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGc                                                                     >  2:725911/1‑140 (MQ=255)                                                                  aCCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGc                                                                     >  2:484710/1‑140 (MQ=255)                                                                  aCCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGc                                                                     >  2:9637/1‑140 (MQ=255)                                                                  aCCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGc                                                                     >  1:259107/1‑140 (MQ=255)                                                                    cGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTg                                                                       >  2:679788/1‑136 (MQ=255)                                                                    cGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTg                                                                       <  1:679788/136‑1 (MQ=255)                                                                      gACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGaa                                                                 >  1:183484/1‑140 (MQ=255)                                                                      gACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGaa                                                                 >  2:918143/1‑140 (MQ=255)                                                                      gACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGaa                                                                 >  1:374588/1‑140 (MQ=255)                                                                      gACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGaa                                                                 >  1:955296/1‑140 (MQ=255)                                                                      gACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCAGTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGaa                                                                 >  2:976375/1‑140 (MQ=255)                                                                       aCTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGaaa                                                                >  2:257113/1‑140 (MQ=255)                                                                        cTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGaaaa                                                               >  1:717372/1‑140 (MQ=255)                                                                          tAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATa                                                             <  1:667309/140‑1 (MQ=255)                                                                                     tCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCAt                                                  >  2:241256/1‑140 (MQ=255)                                                                                     tCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCAt                                                  >  2:318086/1‑140 (MQ=255)                                                                                        aTACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAg                                               <  1:294557/140‑1 (MQ=255)                                                                                        aTACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAg                                               <  1:722667/140‑1 (MQ=255)                                                                                           cAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTg                                            <  1:460846/140‑1 (MQ=255)                                                                                              cGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAg                                                        >  2:1077819/1‑125 (MQ=255)                                                                                              cGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAg                                                        <  1:1077819/125‑1 (MQ=255)                                                                                              cGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGtt                                         <  2:717372/140‑1 (MQ=255)                                                                                              cGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGtt                                         >  1:35380/1‑140 (MQ=255)                                                                                                gTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTg                                       <  2:558552/140‑1 (MQ=255)                                                                                                   cTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAg                                               <  2:371426/129‑1 (MQ=255)                                                                                                   cTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAg                                               >  1:371426/1‑129 (MQ=255)                                                                                                   cTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCaa                                    >  1:277497/1‑140 (MQ=255)                                                                                                   cTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCaa                                    >  1:711529/1‑140 (MQ=255)                                                                                                   cTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGACTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCaa                                    >  2:516677/1‑140 (MQ=255)                                                                                                      gAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAgg                                 >  1:274412/1‑140 (MQ=255)                                                                                                        aGGTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTg                               >  1:893968/1‑140 (MQ=255)                                                                                                          gTACGGATGAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCa                             <  1:918143/140‑1 (MQ=255)                                                                                                                  gAAGAGTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGa                     >  2:786882/1‑140 (MQ=255)                                                                                                                     gagTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAAt                  >  2:791084/1‑140 (MQ=255)                                                                                                                     gagTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAAt                  >  1:498383/1‑140 (MQ=255)                                                                                                                       gTCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGACTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATcc                <  1:516677/140‑1 (MQ=255)                                                                                                                        tCAGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGTGATTCCGTTTGATTTCGAGCGTCACCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAA‑GTGCATTGCAGGAAATCCTc              >  2:449722/1‑140 (MQ=255)                                                                                                                          aGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCa             >  2:777765/1‑140 (MQ=255)                                                                                                                          aGCGCGCTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCa             >  1:1055399/1‑140 (MQ=255)                                                                                                                            cgcgcTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAATGGTGCATTGCAGGaaa                   >  1:81570/1‑132 (MQ=255)                                                                                                                            cgcgcTCGCTGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAATGGTGCATTGCAGGaaa                   <  2:81570/132‑1 (MQ=255)                                                                                                                              cgctcgctGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAAtgt         <  2:1055399/140‑1 (MQ=255)                                                                                                                              cgctcgctGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAAtgt         <  1:725911/140‑1 (MQ=255)                                                                                                                                ctcgctGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAAtgtgt       >  2:780424/1‑140 (MQ=255)                                                                                                                                ctcgctGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAAtgtgt       >  2:861151/1‑140 (MQ=255)                                                                                                                                  cgctGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATgtgtgt     >  1:237267/1‑140 (MQ=255)                                                                                                                                     tGGTCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCg  <  1:777765/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         CCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAAAAAATTGATGAGATTCCGTTTGATTTCGAGCGTCGCCGGATGTCGGTGGTAGTGGCAGAAAATACCGAGCACCATCAGCTGGTTTGCAAAGGTGCATTGCAGGAAATCCTCAATGTGTGTTCG  >  NC_000913/4468790‑4469061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑