Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	365,310	A→G	G332G (GGT→GGC)	lacZ ←	beta‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	365,310	0	A	G	100.0%	293.2 / NA	82	G332G (GGT→GGC)	lacZ	beta‑galactosidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/40);  total (42/40)

GATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGA  >  NC_000913/365175‑365442                                                                                                                                           |                                                                                                                                     gATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGa                                                                                                                                  >  1:819935/1‑140 (MQ=255) gATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGa                                                                                                                                  <  1:169952/140‑1 (MQ=255)   tatCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACa                                                                                                                                >  1:696358/1‑140 (MQ=255)        tGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCa                                                                                                                           <  2:604209/140‑1 (MQ=255)        tGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCa                                                                                                                           <  2:566641/140‑1 (MQ=255)        tGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCa                                                                                                                           >  1:566641/1‑140 (MQ=255)           aCCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCg                                                                                                                             <  2:625288/135‑1 (MQ=255)           aCCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCg                                                                                                                             <  2:286125/135‑1 (MQ=255)           aCCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCg                                                                                                                             >  1:625288/1‑135 (MQ=255)           aCCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCg                                                                                                                             >  1:286125/1‑135 (MQ=255)           aCCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGc                                                                                                                        >  2:67088/1‑140 (MQ=255)           aCCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGc                                                                                                                        >  1:909754/1‑140 (MQ=255)            ccATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCt                                                                                                                       >  2:794686/1‑140 (MQ=255)              aTCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACa                                                                                                                                >  1:287268/1‑129 (MQ=255)              aTCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACa                                                                                                                                <  2:287268/129‑1 (MQ=255)              aTCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTc                                                                                                                     >  1:834331/1‑140 (MQ=255)               tCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGa                                                                                                                                  <  2:732593/126‑1 (MQ=255)               tCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGa                                                                                                                                  >  1:732593/1‑126 (MQ=255)               tCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCt                                                                                                                    >  1:246146/1‑140 (MQ=255)               tCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCt                                                                                                                    >  2:626839/1‑140 (MQ=255)               tCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCt                                                                                                                    >  2:693936/1‑140 (MQ=255)               tCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCt                                                                                                                    >  1:1030490/1‑140 (MQ=255)               tCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCt                                                                                                                    >  1:169368/1‑140 (MQ=255)               tCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCt                                                                                                                    >  2:686038/1‑140 (MQ=255)                cGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCtt                                                                                                                      <  2:384268/140‑1 (MQ=255)                  tCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCt                                                                                                                 <  1:695241/140‑1 (MQ=255)                    tGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTc                                                                                                               <  1:686038/140‑1 (MQ=255)                      cTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCaa                                                                                                             <  1:1023023/140‑1 (MQ=255)                         aTCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCa                                                                                                          >  2:738448/1‑140 (MQ=255)                                  ctgaccATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGt                                                                                                 <  2:696358/140‑1 (MQ=255)                                     accATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTcgg                                                                                              >  2:292719/1‑140 (MQ=255)                                     accATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTcgg                                                                                              >  2:558789/1‑140 (MQ=255)                                     accATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTcgg                                                                                              >  1:405234/1‑140 (MQ=255)                                     accATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTcgg                                                                                              >  1:38137/1‑140 (MQ=255)                                       cATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTcggcg                                                                                            <  2:38137/140‑1 (MQ=255)                                       cATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTcggcg                                                                                            <  1:794686/140‑1 (MQ=255)                                       cATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTcggcg                                                                                            <  2:169368/140‑1 (MQ=255)                                         tGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGgt                                                                                          >  1:907518/1‑140 (MQ=255)                                          gCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGgtg                                                                                         >  1:164315/1‑140 (MQ=255)                                           cAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGTTTCAATCAGCGTGCCGTCGGCGgtgt                                                                                        <  2:819935/140‑1 (MQ=255)                                           cAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGgtgt                                                                                        >  1:191619/1‑140 (MQ=255)                                           cAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGgtgt                                                                                        <  1:738448/140‑1 (MQ=255)                                             gagGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGc                                                                                      <  1:626839/140‑1 (MQ=255)                                                     tgCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAac                                                                              >  2:726231/1‑140 (MQ=255)                                                       cTCGTGACGGTTAACGTCTCGAATCAGCACCGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAacca                                                                            <  2:524994/140‑1 (MQ=255)                                                          gTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCg                                                                         >  2:155975/1‑140 (MQ=255)                                                            gACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCa                                                                       <  2:907518/140‑1 (MQ=255)                                                            gACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCa                                                                       >  2:1048214/1‑140 (MQ=255)                                                            gACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCa                                                                       <  1:1048214/140‑1 (MQ=255)                                                               ggTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGa                                                                    >  1:1005706/1‑140 (MQ=255)                                                               ggTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGa                                                                    >  2:615103/1‑140 (MQ=255)                                                                         tCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCg                                                          >  1:709462/1‑140 (MQ=255)                                                                           gAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCggg                                                        >  2:51899/1‑140 (MQ=255)                                                                                  cAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCgg                                                 <  2:834331/140‑1 (MQ=255)                                                                                        cTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTcc                                           <  2:885825/140‑1 (MQ=255)                                                                                            ccGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCGCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGACTCGGGATTTCGGCGCTCCACAg                                       >  2:1083666/1‑140 (MQ=255)                                                                                                    cagcagcagcagACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCg                                  >  2:584004/1‑140 (MQ=255)                                                                                                           agcagACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTc                           <  1:155975/140‑1 (MQ=255)                                                                                                            gcggACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCg                          >  2:373027/4‑140 (MQ=255)                                                                                                            gcagACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCg                          <  2:254161/140‑1 (MQ=255)                                                                                                             cagACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGa                         <  2:221129/140‑1 (MQ=255)                                                                                                             cagACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGCGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGa                         <  2:219946/140‑1 (MQ=255)                                                                                                               gACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACg                       >  1:376394/1‑140 (MQ=255)                                                                                                               gACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACg                       >  1:1001433/1‑140 (MQ=255)                                                                                                               gACCATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACg                       <  1:584004/140‑1 (MQ=255)                                                                                                                 ccATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGtt                     <  1:558789/140‑1 (MQ=255)                                                                                                                 ccATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGtt                     <  1:726231/140‑1 (MQ=255)                                                                                                                 ccATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGtt                     >  1:889406/1‑140 (MQ=255)                                                                                                                 ccATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGtt                     <  1:292719/140‑1 (MQ=255)                                                                                                                  cATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCTGCGCTCCACAGTTTCGGGTTTTCGACGTTc                    <  2:1001433/140‑1 (MQ=255)                                                                                                                  cATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTc                    <  2:376394/140‑1 (MQ=255)                                                                                                                  cATTTTCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTc                    <  2:164315/140‑1 (MQ=255)                                                                                                                    ttttCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAg                  >  1:993434/1‑140 (MQ=255)                                                                                                                    ttttCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAg                  <  1:693936/140‑1 (MQ=255)                                                                                                                      ttCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGAc                <  1:1063288/140‑1 (MQ=255)                                                                                                                       tCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACg               <  1:615103/140‑1 (MQ=255)                                                                                                                       tCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACg               <  1:51899/140‑1 (MQ=255)                                                                                                                       tCAATCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACg               <  2:909754/140‑1 (MQ=255)                                                                                                                           tCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACg               >  1:925825/1‑136 (MQ=255)                                                                                                                           tCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACg               <  2:925825/136‑1 (MQ=255)                                                                                                                           tCCGCACCTCGCGGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAgt           <  2:191619/140‑1 (MQ=255)                                                                                                                                    cgcgGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGa  >  2:1028474/1‑140 (MQ=255)                                                                                                                                    cgcgGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGa  >  1:139095/1‑140 (MQ=255)                                                                                                                                    cgcgGAAGCCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGa  <  2:889406/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                     GATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTT‑‑‑CAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGA  >  NC_000913/365175‑365442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑