Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,610,444	A→G	D344G (GAC→GGC)	prfC →	peptide chain release factor RF3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,610,444	0	A	G	100.0%	210.7 / NA	58	D344G (GAC→GGC)	prfC	peptide chain release factor RF3
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/35);  total (23/35)

TCGTATTTAAAATTCAGGCCAACATGGACCCGAAACACCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGC‑GATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCG  >  NC_000913/4610309‑4610578                                                                                                                                        |                                                                                                                                        tCGTATTTAAAATTCAGGCCAACATGGACCCGAAACACCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGcgcg                                                                                                                                     >  2:51066/1‑140 (MQ=255)   gTATTTAAAATTCAGGCCAACATGGACCCGAAACACCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCt                                                                                                                                   <  1:13010/140‑1 (MQ=255)           aaTTCAGGCCAACATGGACCCGAAACACCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTa                                                                                                                           <  1:371277/140‑1 (MQ=255)              tCAGGCCAACATGGACCCGAAACACCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATgg                                                                                                                        >  1:634924/1‑140 (MQ=255)                aGGCCAACATGGACCCGAAACACCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCg                                                                                                                      >  1:367381/1‑140 (MQ=255)                 ggCCAACATGGACCCGAAACACCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTa                                                                                                                           <  2:373293/134‑1 (MQ=255)                 ggCCAACATGGACCCGAAACACCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTa                                                                                                                           >  1:373293/1‑134 (MQ=255)                    cAACATGGACCCGAAACACCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGTg                                                                                                                  <  1:64131/140‑1 (MQ=255)                            aCCCGAAACACCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTa                                                                                                                           >  2:278164/1‑123 (MQ=255)                            aCCCGAAACACCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTa                                                                                                                           <  1:278164/123‑1 (MQ=255)                            aCCCGAAACACCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGTGACCGTTCg                                                                                                          >  1:394521/1‑140 (MQ=255)                            aCCCGAAACACCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGTGACCGTTCg                                                                                                          <  1:403535/140‑1 (MQ=255)                            aCCCGAAACACCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGTGACCGTTCg                                                                                                          <  2:536690/140‑1 (MQ=255)                            aCCCGAAACACCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGTGACCGTTCg                                                                                                          >  1:239424/1‑140 (MQ=255)                            aCCCGAAACACCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGTGACCGTTCg                                                                                                          <  2:367381/140‑1 (MQ=255)                              ccGAAACACCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGTGACCGTTCGCa                                                                                                        <  2:634924/140‑1 (MQ=255)                              ccGAAACACCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGTGACCGTTCGCa                                                                                                        <  2:239424/140‑1 (MQ=255)                              ccGAAACACCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGTGACCGTTCGCa                                                                                                        <  1:385964/140‑1 (MQ=255)                                 aaaCACCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGGGGCCGGTCGCACgg                                                                                                     >  1:659370/1‑139 (MQ=255)                                           accgcgTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGTGAACGGTCCCGCGGTGaagaagag                                                                                           >  1:796834/1‑138 (MQ=255)                                                           gCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGC‑GATATcc                                                                          <  2:394521/140‑1 (MQ=255)                                                            cGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGC‑GATATCCt                                                                         <  2:458550/140‑1 (MQ=255)                                                              tgtgGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGC‑GATATCCTCg                                                                       <  1:999201/140‑1 (MQ=255)                                                                tggtgTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGC‑GATATCCTCGGc                                                                     <  2:583079/140‑1 (MQ=255)                                                                tggtgTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGC‑GATATCCTCGGc                                                                     <  1:509625/140‑1 (MQ=255)                                                                tggtgTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGGGACCATTCGCACGTTGTAGAAGCGTATCCGGGC‑GGTATCCCCGGc                                                                     >  2:100685/1‑140 (MQ=255)                                                                 ggtgTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGC‑GATATCCTCGGcc                                                                    <  1:806415/140‑1 (MQ=255)                                                                 ggtgTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGGGAGCGTTCGCGCGGTGCAGAAACGGATCCGGGC‑GGCCTCCTCTGcc                                                                    >  2:509625/1‑140 (MQ=255)                                                                  gtgtCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGGGGCCGCTCGCACGGTGTAGAAAGGTATTCCGGC‑GGTATCCTCCGccc                                                                   >  2:701176/1‑139 (MQ=255)                                                                    gtCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGTGGCCGGTCGCACGCTGAAGAAAAGGATCCCGGC‑GGTATCCTccgcccgc                                                                 >  2:406773/1‑140 (MQ=255)                                                                      ccGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGC‑GATATCCTCGGCCTGcac                                                               <  2:85577/140‑1 (MQ=255)                                                                       cGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGTGGCCGGTCGCACGCTGGAGAAGAGGATCCGGGC‑GGGATTCTCGGCCTGcccc                                                              >  2:912305/1‑137 (MQ=255)                                                                         gTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGC‑GATATCCTCGGCCTGCACAAc                                                            <  1:1038256/140‑1 (MQ=255)                                                                         gTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGGGGGCGGTCGCACGTTGGAGAAGCGGATCCCGGC‑GGTATTCTCGGCCCGCCCAAc                                                            >  2:436573/1‑140 (MQ=255)                                                                           aaaTATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGC‑GATATCCTCGGCCTGCACAACCa                                                          <  1:268197/140‑1 (MQ=255)                                                                              tatGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGC‑GATATCCTCGGCCTGCACAACCACgg                                                       <  1:701176/140‑1 (MQ=255)                                                                               atGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGC‑GATATCCTCGGCCTGCACAACCACGGc                                                      <  2:205894/140‑1 (MQ=255)                                                                                    aaaGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGGAGAAGCGGATCCGGGC‑GGTATCCTCGGCCTGCACAACCACCGCAccct                                                 >  2:371584/1‑138 (MQ=255)                                                                                    aaaGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGGGGGCGGTCGCCCGGTGGAGAAAAGGATCCGGGC‑GGTATCCTCCGCCCGCCCAACCACCGCACCaa                                                 >  2:667662/1‑139 (MQ=255)                                                                                      aGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGGGGGCCCTTGCACGGTGTAGGAACGGATACGGGG‑GGGATCCTCCGCCCGCCCCACCACCGcccccatc                                               >  2:268197/1‑136 (MQ=255)                                                                                         cATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGC‑GATATCCTCGGCCTGCACAACCACGGCACCATTCAGa                                            <  1:371584/140‑1 (MQ=255)                                                                                         cATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGC‑GATATCCTCGGCCTGCACAACCACGGCACCATTCAGa                                            <  1:51066/140‑1 (MQ=255)                                                                                         cATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGGGGCGGTTCGCACGCTGCAGAAACGGATCCGGGC‑GGGATCCTCCGCCCGCCCCACCACCGCACCCCTCAGa                                            >  2:950649/1‑140 (MQ=255)                                                                                                cTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGC‑GATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGa                                     <  1:912305/140‑1 (MQ=255)                                                                                                cTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGC‑GATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGa                                     <  1:667662/140‑1 (MQ=255)                                                                                                cTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGC‑GATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGa                                     <  1:6540/140‑1 (MQ=255)                                                                                                cTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGC‑GATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGa                                     <  2:244801/140‑1 (MQ=255)                                                                                                cTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGC‑GATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGa                                     <  2:245327/140‑1 (MQ=255)                                                                                                cTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGC‑GATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGa                                     <  2:291251/140‑1 (MQ=255)                                                                                                      cAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGC‑GATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCtt                               <  1:793552/140‑1 (MQ=255)                                                                                                        ggTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGGGGGCGTTCGCCCGGTGAAGAAAAGGAACCCGGG‑GGTATTCTCGGCCTGCCCCACCACCGCCCCAATCCAATCGGCGGCACCcttt                             >  2:535542/1‑139 (MQ=255)                                                                                                        ggTGCGCACTGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGGGGCCGGTTGCACGATGGAGGAGAGGATACCGGG‑GAGAATCTCCGCCCGCCCCACCACAGCACCCTTCAGATCCAGGACACCcttt                             >  2:293391/1‑139 (MQ=255)                                                                                                                 tGCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGC‑GATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTg                    <  1:436573/140‑1 (MQ=255)                                                                                                                  gCGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGGGACCGTTCGCACGTTGAAGAAGCGTATCCGGGC‑GGTATCCTCGGCCTGCACAACCACCGCACCATTCAGATCGGCGGCACCCTTACCCCgggggg                   >  2:474309/1‑137 (MQ=255)                                                                                                                   cGAAAGATGTGGTGATCTCCGGCGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGC‑GATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTgag                  <  1:702976/140‑1 (MQ=255)                                                                                                                              gtgATCTCCGGCGCGCTGACCTTTATGGCGGGTGACCATTCGCACGTTGAAGAAGCGTATCCGGGC‑GATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGtt       <  1:100685/140‑1 (MQ=255)                                                                                                                                   ctcCGGCGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGC‑GATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCg  <  2:659370/140‑1 (MQ=255)                                                                                                                                   ctcCGGCGCGCTGACCTTTATGGCGGGGGGGCCCTCGCCCGGTGTAAAAAAGAATACGGGCGGGGATCC‑CGGCCTGCCCAACCCCGGGACCCTTTCAATCGGCGACACCCCTACCCCCGGGGGGAAGATGAAGGTCACCg  >  2:798898/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        TCGTATTTAAAATTCAGGCCAACATGGACCCGAAACACCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGC‑GATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCG  >  NC_000913/4610309‑4610578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑