| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,632,411 | A→G | Y560C (TAC→TGC) | slt → | soluble lytic murein transglycosylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,632,411 | 0 | A | G | 96.8% | 208.1 / ‑2.0 | 62 | Y560C (TAC→TGC) | slt | soluble lytic murein transglycosylase |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (35/25); total (36/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01 | |||||||||||
GTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGA > NC_000913/4632278‑4632543 | gTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCa < 1:712722/140‑1 (MQ=255) gCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGt > 2:336260/1‑140 (MQ=255) gCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGt > 1:310501/1‑140 (MQ=255) ccAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTAc < 2:91508/128‑1 (MQ=255) ccAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTAc > 1:91508/1‑128 (MQ=255) gCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATcag < 1:440179/140‑1 (MQ=255) cTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATcagc > 2:419032/1‑140 (MQ=255) tGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATcagcag > 1:821960/1‑140 (MQ=255) tATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCaata < 2:979518/140‑1 (MQ=255) ggTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCa > 2:603237/1‑130 (MQ=255) ggTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCa < 1:603237/130‑1 (MQ=255) ggTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATc < 2:65216/136‑1 (MQ=255) ggTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATc > 1:65216/1‑136 (MQ=255) ggTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTAt > 2:13545/1‑140 (MQ=255) gTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTAtt > 2:850960/1‑140 (MQ=255) gTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTAtt > 1:593180/1‑140 (MQ=255) ccATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTcag < 2:252814/140‑1 (MQ=255) aaGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATaa > 1:426662/1‑140 (MQ=255) aaGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATaa > 2:93779/1‑140 (MQ=255) aaGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATaa > 2:575877/1‑140 (MQ=255) aaGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATaa > 2:691923/1‑140 (MQ=255) aTGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGc < 1:575877/140‑1 (MQ=255) ctctATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGGACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGAc > 2:1025367/1‑140 (MQ=255) ctctATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGAc > 2:581441/1‑140 (MQ=255) ctctATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGAc < 1:336260/140‑1 (MQ=255) ctctATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGAc > 2:512224/1‑140 (MQ=255) tctATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGAcc < 1:512224/140‑1 (MQ=255) ggTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGt > 1:356053/1‑140 (MQ=255) ggTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGt < 1:839485/140‑1 (MQ=255) ggTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGt > 1:914461/1‑140 (MQ=255) ttATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGc > 2:496480/1‑140 (MQ=255) taGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGaa > 2:535219/1‑140 (MQ=255) taGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGaa > 1:325333/1‑140 (MQ=255) aGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAAc < 1:535219/140‑1 (MQ=255) cAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCt < 2:356053/140‑1 (MQ=255) cAGTCCTGGACAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCt < 2:214125/140‑1 (MQ=255) tCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGc > 2:141652/1‑140 (MQ=255) tCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGc > 2:373365/1‑140 (MQ=255) ccTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCt > 1:78697/1‑140 (MQ=255) ccTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCt > 1:545336/1‑140 (MQ=255) ccTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCt > 1:222514/1‑140 (MQ=255) ccTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCt > 2:479409/1‑140 (MQ=255) ttGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAg > 2:1082696/1‑140 (MQ=255) ttGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAg > 2:497253/1‑140 (MQ=255) ttGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAg > 2:95260/1‑140 (MQ=255) ggATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAgcg < 1:728681/137‑1 (MQ=255) ggATCCGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAgcg > 2:728681/1‑137 (MQ=255) ccGGAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCg < 2:545336/140‑1 (MQ=255) gAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTAt < 1:1025367/140‑1 (MQ=255) gAAACGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTAt < 1:581441/140‑1 (MQ=255) aCGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGa < 1:496480/140‑1 (MQ=255) aCGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGa < 1:419032/140‑1 (MQ=255) cGAATATCAACATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGAc < 2:310501/140‑1 (MQ=255) aCATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCa < 1:141652/140‑1 (MQ=255) aCATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCa < 1:373365/140‑1 (MQ=255) cATTGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCAt < 1:13545/140‑1 (MQ=255) tGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTg > 2:601192/1‑140 (MQ=255) tGGCACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTg > 1:481704/1‑140 (MQ=255) cACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCAt < 2:1053599/134‑1 (MQ=255) cACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCAt > 1:1053599/1‑134 (MQ=255) cACCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCg > 1:673229/1‑140 (MQ=255) aCCAGTTGCCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCga < 1:479409/140‑1 (MQ=255) | GTAGGGGCCAGCGGCTTGATGCAGATTATGCCTGGTACAGCGACCCATACGGTGAAGATGTTCTCTATTCCCGGTTATAGCAGTCCTGGGCAATTGCTGGATCCGGAAACGAATATCAACATTGGCACCAGTTACCTGCAATATGTTTATCAGCAGTTTGGCAATAATCGTATTTTCTCCTCAGCAGCTTATAACGCCGGACCAGGGCGGGTGCGAACCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGA > NC_000913/4632278‑4632543 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |