Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	483,232	T→C	N391S (AAC→AGC)	acrB ←	multidrug efflux pump RND permease AcrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	483,232	0	T	C	100.0%	253.6 / NA	70	N391S (AAC→AGC)	acrB	multidrug efflux pump RND permease AcrB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/40);  total (30/40)

CCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGC  >  NC_000913/483096‑483365                                                                                                                                         |                                                                                                                                      ccccATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTa                                                                                                                                    >  2:366017/1‑140 (MQ=255) ccccATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATACCGAACATTGTTAGCGTGCTTa                                                                                                                                    >  1:774138/1‑140 (MQ=255)     aTCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCga                                                                                                                                >  2:167338/1‑140 (MQ=255)           ttACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGcc                                                                                                                          >  2:25033/1‑140 (MQ=255)              cGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCaaa                                                                                                                       <  1:679007/140‑1 (MQ=255)                ggTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGctt                                                                                                                                     >  2:480907/1‑124 (MQ=255)                    gCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCga                                                                                                                                <  2:112897/125‑1 (MQ=255)                    gCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCga                                                                                                                                >  1:112897/1‑125 (MQ=255)                    gCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAggcggc                                                                                                                 <  2:244682/140‑1 (MQ=255)                    gCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAggcggc                                                                                                                 >  1:48919/1‑140 (MQ=255)                       tcttTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAg                                                                                                              >  1:173542/1‑140 (MQ=255)                       tcttTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAg                                                                                                              <  1:57667/140‑1 (MQ=255)                          tttGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGAc                                                                                                           >  2:662332/1‑140 (MQ=255)                          tttGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGAc                                                                                                           <  2:756399/140‑1 (MQ=255)                               cggcAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCaa                                                                                                      <  1:662332/140‑1 (MQ=255)                                    aaCCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTc                                                                                                 <  1:81672/140‑1 (MQ=255)                                    aaCCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTc                                                                                                 <  1:962974/140‑1 (MQ=255)                                    aaCCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTc                                                                                                 <  2:173542/140‑1 (MQ=255)                                     cccTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGTGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTcc                                                                                                <  2:192045/139‑1 (MQ=255)                                       cttcttCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCg                                                                                              <  1:25033/140‑1 (MQ=255)                                                ccATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATacc                                                                                     >  2:739896/1‑140 (MQ=255)                                                ccATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATacc                                                                                     <  1:793587/140‑1 (MQ=255)                                                  aTAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATaccac                                                                                   <  1:980475/140‑1 (MQ=255)                                                          cTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATaccac                                                                                   <  1:225371/132‑1 (MQ=255)                                                          cTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATaccac                                                                                   >  2:225371/1‑132 (MQ=255)                                                           tCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGc                                                                          >  2:732489/1‑140 (MQ=255)                                                           tCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGc                                                                          >  2:153355/1‑140 (MQ=255)                                                             aaCGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCaa                                                                        <  1:509874/140‑1 (MQ=255)                                                             aaCGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCaa                                                                        >  1:36559/1‑140 (MQ=255)                                                             aaCGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCaa                                                                        <  1:732489/140‑1 (MQ=255)                                                               cGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATg                                                                      >  1:12252/1‑140 (MQ=255)                                                                gTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATgg                                                                     <  2:668605/140‑1 (MQ=255)                                                                    tCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCgg                                                                 <  2:764731/140‑1 (MQ=255)                                                                     cTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGa                                                                >  1:87863/1‑140 (MQ=255)                                                                       aCCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAAt                                                              >  1:260364/1‑140 (MQ=255)                                                                             aCGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGt                                                        <  2:260364/140‑1 (MQ=255)                                                                              cGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTc                                                       >  1:75417/1‑140 (MQ=255)                                                                                 tGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTcgcg                                                    <  1:167338/140‑1 (MQ=255)                                                                                    cGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCgg                                                 <  2:75417/140‑1 (MQ=255)                                                                                    cGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCgg                                                 <  2:36559/140‑1 (MQ=255)                                                                                        aTCCACCAACAGGCCGATGGCGAGCACCATCCCTAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGt                                             <  2:804232/140‑1 (MQ=255)                                                                                         tCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGtt                                            >  1:384386/1‑140 (MQ=255)                                                                                             ccaACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGc                                        <  1:318185/140‑1 (MQ=255)                                                                                              caACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCa                                       <  2:384386/140‑1 (MQ=255)                                                                                                aCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAgg                                     <  2:136464/140‑1 (MQ=255)                                                                                                 cAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGa                                    >  2:42153/1‑140 (MQ=255)                                                                                                   ggCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAAc                                  <  1:366017/140‑1 (MQ=255)                                                                                                    gCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACa                                 <  2:87863/140‑1 (MQ=255)                                                                                                    gCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACa                                 >  2:973534/1‑140 (MQ=255)                                                                                                    gCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACa                                 >  2:615010/1‑140 (MQ=255)                                                                                                    gCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACa                                 <  1:153355/140‑1 (MQ=255)                                                                                                    gCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACa                                 <  2:19943/140‑1 (MQ=255)                                                                                                    gCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACa                                 >  1:19943/1‑140 (MQ=255)                                                                                                    gCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACa                                 >  1:27204/1‑140 (MQ=255)                                                                                                    gCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACa                                 >  1:777345/1‑140 (MQ=255)                                                                                                     ccGATGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAg                                <  2:110776/140‑1 (MQ=255)                                                                                                        aTGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATa                             >  2:425228/1‑140 (MQ=255)                                                                                                         tGGCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATAc                            <  2:27204/140‑1 (MQ=255)                                                                                                          ggCGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACa                           <  1:425228/140‑1 (MQ=255)                                                                                                            cGAGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATa                         <  1:739896/140‑1 (MQ=255)                                                                                                              aGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCGGGAACAGATACATAAc                       <  2:12252/140‑1 (MQ=255)                                                                                                              aGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGAt                              >  1:285339/1‑133 (MQ=255)                                                                                                              aGCACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGAt                              <  2:285339/133‑1 (MQ=255)                                                                                                                cACCATCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCa                     >  2:193057/1‑140 (MQ=255)                                                                                                                     tCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAac                <  1:973534/140‑1 (MQ=255)                                                                                                                     tCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAac                <  1:42153/140‑1 (MQ=255)                                                                                                                     tCCCGAACATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAac                <  1:222043/140‑1 (MQ=255)                                                                                                                          aaCATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg           <  2:777345/140‑1 (MQ=255)                                                                                                                           aCATTGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAgg          >  1:979429/1‑140 (MQ=255)                                                                                                                             aTTGTTAGCGTGCTTATCGAGGAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgat        >  2:824008/1‑140 (MQ=255)                                                                                                                               tGTTAGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatga      >  1:396542/1‑140 (MQ=255)                                                                                                                                   aGCGTGCTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGc  >  2:780150/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      CCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGC  >  NC_000913/483096‑483365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑