Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	40,906	A→G	R342R (CGT→CGC)	caiT ←	L‑carnitine:gamma‑butyrobetaine antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	40,906	0	A	G	100.0%	288.4 / NA	79	R342R (CGT→CGC)	caiT	L‑carnitine:gamma‑butyrobetaine antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/41);  total (38/41)

TACTGTTCGATCAGATTTGGAATGTTGATGATGTTTTTATCTATCAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGC  >  NC_000913/40769‑41041                                                                                                                                          |                                                                                                                                        tACTGTTCGATCAGATTTGGAATGTTGATGATGTTTTTATCTATCAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCg                                                                                                                                       >  2:91397/1‑140 (MQ=255)   cTGTTCGATCAGATTTGGAATGTTGATGATGTTTTTATCTATCAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGAc                                                                                                                                     <  1:375639/140‑1 (MQ=255)    tGTTCGATCAGATTTGGAATGTTGATGATGTTTTTATCTATCAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGAcc                                                                                                                                    >  2:529836/1‑140 (MQ=255)    tGTTCGATCAGATTTGGAATGTTGATGATGTTTTTATCTATCAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGAc                                                                                                                                     <  2:499790/139‑1 (MQ=255)    tGTTCGATCAGATTTGGAATGTTGATGATGTTTTTATCTATCAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGAc                                                                                                                                     >  1:499790/1‑139 (MQ=255)      ttCGATCAGATTTGGAATGTTGATGATGTTTTTATCTATCAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCAc                                                                                                                                  >  2:120475/1‑140 (MQ=255)      ttCGATCAGATTTGGAATGTTGATGATGTTTTTATCTATCAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCAc                                                                                                                                  >  2:16945/1‑140 (MQ=255)             aGATTTGGAATGTTGATGATGTTTTTATCTATCAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGAt                                                                                                                           <  2:1014505/140‑1 (MQ=255)                 ttGGAATGTTGATGATGTTTTTATCTATCAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCgg                                                                                                                       <  1:529836/140‑1 (MQ=255)                   ggAATGTTGATGATGTTTTTATCTATCAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGc                                                                                                                     <  2:885488/140‑1 (MQ=255)                         ttGATGATGTTTTTATCTATCAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGTGACCACGGGAGATGCGGGCGAGGaa                                                                                                               >  2:306904/1‑140 (MQ=255)                         ttGATGATGTTTTTATCTATCAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGaa                                                                                                               >  1:530638/1‑140 (MQ=255)                         ttGATGATGTTTTTATCTATCAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGaa                                                                                                               >  2:81461/1‑140 (MQ=255)                           gatgatGTTTTTATCTATCAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGa                                                                                                             <  2:236401/140‑1 (MQ=255)                             tgatgTTTTTATCTATCAACAGCAGAGTGTTCCTACCGAGTACAGTTCACAGGATCCAGATTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATa                                                                                                           <  2:622378/140‑1 (MQ=255)                             tgatgTTTTTATCTATCAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATa                                                                                                           <  1:431060/140‑1 (MQ=255)                              gatgTTTTTATCTATCAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATAc                                                                                                          >  1:533646/1‑140 (MQ=255)                                    tttATCTATCAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCt                                                                                                    >  1:151145/1‑140 (MQ=255)                                       atctatcAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGa                                                                                                 <  2:530638/140‑1 (MQ=255)                                              aaCAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGTGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATa                                                                                          <  1:306904/140‑1 (MQ=255)                                                   cagAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATca                                                                                     >  1:64694/1‑140 (MQ=255)                                                   cagAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATca                                                                                     >  2:846823/1‑140 (MQ=255)                                                    agagTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATcac                                                                                    <  2:457592/140‑1 (MQ=255)                                                      agTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATcaccc                                                                                  >  2:433431/1‑140 (MQ=255)                                                      agTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATcaccc                                                                                  >  1:869648/1‑140 (MQ=255)                                                      agTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATcaccc                                                                                  >  1:1082549/1‑140 (MQ=255)                                                      agTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATcaccc                                                                                  >  2:192263/1‑140 (MQ=255)                                                       gtgtTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATcaccca                                                                                 <  1:91397/140‑1 (MQ=255)                                                        tgtTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGTGGGCGAGGAAGATACTCATCTGGATAGCATAAATcacccac                                                                                >  1:95985/1‑140 (MQ=255)                                                         gtTACTACTGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATcacccacc                                                                               <  1:433431/140‑1 (MQ=255)                                                         gtTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATcacccacc                                                                               <  1:921900/140‑1 (MQ=255)                                                         gtTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATcacccacc                                                                               <  1:16945/140‑1 (MQ=255)                                                           tactacCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCAt                                                                             >  2:279082/1‑140 (MQ=255)                                                            actacCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATg                                                                            <  2:1082549/140‑1 (MQ=255)                                                              tacCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGcc                                                                          <  1:846823/140‑1 (MQ=255)                                                              tacCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGcc                                                                          <  1:279082/140‑1 (MQ=255)                                                              tacCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGcc                                                                          <  1:192263/140‑1 (MQ=255)                                                                  gAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGt                                                                      >  2:669253/1‑140 (MQ=255)                                                                   aGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTa                                                                     <  1:120475/140‑1 (MQ=255)                                                                    gTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAg                                                                    >  1:691059/1‑140 (MQ=255)                                                                    gTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAg                                                                    >  2:811020/1‑140 (MQ=255)                                                                     tACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTata                                                                   >  2:375599/1‑138 (MQ=255)                                                                        aGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGTGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAaca                                                                <  2:95985/140‑1 (MQ=255)                                                                        aGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAaca                                                                <  2:138172/140‑1 (MQ=255)                                                                        aGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAaca                                                                <  1:811020/140‑1 (MQ=255)                                                                        aGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAaca                                                                >  2:352340/1‑140 (MQ=255)                                                                        aGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAaca                                                                <  2:253466/140‑1 (MQ=255)                                                                        aGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAaca                                                                <  2:232191/140‑1 (MQ=255)                                                                          tCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGcc                                                                          <  1:63787/128‑1 (MQ=255)                                                                          tCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACg                                                              >  1:2119/1‑140 (MQ=255)                                                                          tCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACg                                                              >  1:355202/1‑140 (MQ=255)                                                                          cccACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGcc                                                                          >  2:63787/2‑128 (MQ=255)                                                                             acaGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTc                                                           <  2:64694/140‑1 (MQ=255)                                                                               aGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCa                                                         >  2:928255/1‑140 (MQ=255)                                                                                  aTCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGcc                                                      <  1:669253/140‑1 (MQ=255)                                                                                    ccAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCt                                                    >  1:457783/1‑140 (MQ=255)                                                                                            aCGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAg                                            >  2:227269/1‑140 (MQ=255)                                                                                             cGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAgc                                           <  2:355202/140‑1 (MQ=255)                                                                                                  tCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTATAACACGGTCCAGCCCTGCGGGAAGCCGCCt                                      <  1:375599/140‑1 (MQ=255)                                                                                                   cAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCtt                                     >  1:715385/1‑140 (MQ=255)                                                                                                      ccccAGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAg                                  >  2:1076452/1‑140 (MQ=255)                                                                                                          aGCACCATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGAt                              <  1:928255/140‑1 (MQ=255)                                                                                                               cATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGAt                         <  1:983179/140‑1 (MQ=255)                                                                                                               cATGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGAt                         <  2:533646/140‑1 (MQ=255)                                                                                                                aTGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGAt                         >  1:521820/1‑139 (MQ=255)                                                                                                                aTGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGAt                         <  2:521820/139‑1 (MQ=255)                                                                                                                 tGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGAt                         >  1:526475/1‑138 (MQ=255)                                                                                                                 tGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGAt                         <  2:526475/138‑1 (MQ=255)                                                                                                                 tGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGAt                         <  2:1049314/138‑1 (MQ=255)                                                                                                                 tGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGAt                         >  1:1049314/1‑138 (MQ=255)                                                                                                                 tGCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCg                       >  2:83419/1‑140 (MQ=255)                                                                                                                  gCCGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCgg                      <  1:227269/140‑1 (MQ=255)                                                                                                                   ccGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGt                     <  2:715385/140‑1 (MQ=255)                                                                                                                    cGAAGCACAGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGta                    <  1:83419/140‑1 (MQ=255)                                                                                                                           caGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGAt                         <  2:865782/128‑1 (MQ=255)                                                                                                                           caGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGAt                         >  1:865782/1‑128 (MQ=255)                                                                                                                           caGTTCACGCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGaaca             >  1:88900/1‑140 (MQ=255)                                                                                                                                   gCACAGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATgcgc     >  1:159791/1‑140 (MQ=255)                                                                                                                                    cacaGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGcgcg    <  2:869648/140‑1 (MQ=255)                                                                                                                                      caGTGCGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGc  <  2:691059/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        TACTGTTCGATCAGATTTGGAATGTTGATGATGTTTTTATCTATCAACAGCAGAGTGTTACTACCGAGTACAGTCCACAGGATCCAGGTTGACGCTGTCAGCCCCAGCACCATGCCGAAGCACAGTTCACGCACAGTACGACCACGGGAGATGCGGGCGAGGAAGATACTCATCTGGATAGCATAAATCACCCACCATGCCCAGTAGAACACGGTCCAGCCCTGCGGGAAGCCGCCTTTAGCGATGGGATCGGTATAGAACAACATGCGCGGC  >  NC_000913/40769‑41041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑