Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	502,496	A→G	V248A (GTC→GCC)	ybaL ←	putative transporter YbaL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	502,496	0	A	G	100.0%	205.4 / NA	57	V248A (GTC→GCC)	ybaL	putative transporter YbaL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/25);  total (32/25)

ACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGACATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGC  >  NC_000913/502362‑502627                                                                                                                                       |                                                                                                                                    acaacaTCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCa                                                                                                                                <  1:492216/140‑1 (MQ=255)  caacaTCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCaa                                                                                                                               <  2:160174/140‑1 (MQ=255)         ccGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGctc                                                                                                                        <  1:205533/140‑1 (MQ=255)           gACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTa                                                                                                                      >  1:184011/1‑140 (MQ=255)            aCGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTAc                                                                                                                     <  1:180502/140‑1 (MQ=255)            aCGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTAc                                                                                                                     >  2:525378/1‑140 (MQ=255)                agaCAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCa                                                                                                                 <  2:1067225/140‑1 (MQ=255)                agaCAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCa                                                                                                                 >  2:921026/1‑140 (MQ=255)                agaCAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCa                                                                                                                 >  1:433223/1‑140 (MQ=255)                  aCAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCAcc                                                                                                               >  1:972934/1‑140 (MQ=255)                         aCAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCa                                                                                                        <  2:433223/140‑1 (MQ=255)                          cAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCaa                                                                                                       <  1:994252/140‑1 (MQ=255)                          cAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCaa                                                                                                       >  1:279385/1‑140 (MQ=255)                             cACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCa                                                                                                        >  2:258238/1‑136 (MQ=255)                             cACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCa                                                                                                        <  1:258238/136‑1 (MQ=255)                             cACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAAccc                                                                                                    >  1:669264/1‑140 (MQ=255)                               ccGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCt                                                                                                  <  1:525378/140‑1 (MQ=255)                                  cAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAAc                                                                                               >  2:379896/1‑140 (MQ=255)                                         gTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAAcgccagcg                                                                                        <  2:184011/140‑1 (MQ=255)                                          tCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAAcgccagcgc                                                                                       >  2:701582/1‑140 (MQ=255)                                                  tGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCg                                                                               <  2:972934/140‑1 (MQ=255)                                                  tGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCg                                                                               <  2:669264/140‑1 (MQ=255)                                                    gCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGAc                                                                             <  1:561421/140‑1 (MQ=255)                                                    gCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGAc                                                                             <  1:701582/140‑1 (MQ=255)                                                    gCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGAc                                                                             <  2:279385/140‑1 (MQ=255)                                                    gCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGAc                                                                             <  1:379896/140‑1 (MQ=255)                                                       gCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAgg                                                                          >  1:1009293/1‑140 (MQ=255)                                                          tATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCg                                                                               <  1:454360/132‑1 (MQ=255)                                                          tATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCg                                                                               >  2:454360/1‑132 (MQ=255)                                                          tATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTa                                                                       >  1:374783/1‑140 (MQ=255)                                                            tCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTaaa                                                                     >  2:924751/1‑140 (MQ=255)                                                               tGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAg                                                                  <  2:526342/140‑1 (MQ=255)                                                                    ggcACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTcgc                                                             >  2:212922/1‑140 (MQ=255)                                                                     gcATGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTcgcg                                                            >  1:914909/1‑140 (MQ=255)                                                                      cACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCga                                                           >  1:51820/1‑140 (MQ=255)                                                                      cACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGCAAACAGCGCGCga                                                           >  2:568905/1‑140 (MQ=255)                                                                        cGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCgaga                                                         >  1:329484/1‑140 (MQ=255)                                                                             aCTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCgg                                                    <  2:914909/140‑1 (MQ=255)                                                                                   ttCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCcg                                              >  1:23930/1‑140 (MQ=255)                                                                                    tCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCcgc                                             <  2:329484/140‑1 (MQ=255)                                                                                     cAGACTCGTTCAGTCCCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCcgcg                                            <  2:793224/140‑1 (MQ=255)                                                                                           cGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGt                                      >  2:793449/1‑140 (MQ=255)                                                                                             ttCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAGAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGc                                    >  2:531653/1‑140 (MQ=255)                                                                                             ttCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGc                                    >  2:996532/1‑140 (MQ=255)                                                                                             ttCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGc                                    >  1:571154/1‑140 (MQ=255)                                                                                             ttCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGc                                    >  1:399591/1‑140 (MQ=255)                                                                                                      aTCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCa                           >  1:9458/1‑140 (MQ=255)                                                                                                      aTCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCa                           <  2:571154/140‑1 (MQ=255)                                                                                                      aTCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCa                           >  1:800752/1‑140 (MQ=255)                                                                                                       tCCCGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCAc                          >  1:831328/1‑140 (MQ=255)                                                                                                        cccGGCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACg                         <  1:924751/140‑1 (MQ=255)                                                                                                            gCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCAc                     >  2:758999/1‑140 (MQ=255)                                                                                                            gCAAAGAACGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCAc                     >  1:851761/1‑140 (MQ=255)                                                                                                                    cGCACCGAGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGc             <  1:921026/140‑1 (MQ=255)                                                                                                                           aGTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTAc      <  2:1009293/140‑1 (MQ=255)                                                                                                                            gTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTAcc     >  2:775853/1‑140 (MQ=255)                                                                                                                            gTGCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTAcc     >  1:1064719/1‑140 (MQ=255)                                                                                                                              gCAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAg   <  1:758999/140‑1 (MQ=255)                                                                                                                               cAAAGGAGGCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGc  >  2:96055/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                    ACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGACATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGC  >  NC_000913/502362‑502627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑