Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	527,730	A→G	V50V (GTA→GTG)	ybbC →	PF15631 family protein YbbC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	527,730	0	A	G	100.0%	262.0 / NA	72	V50V (GTA→GTG)	ybbC	PF15631 family protein YbbC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/39);  total (33/39)

CAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTAAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGCAAAGTTAC  >  NC_000913/527594‑527866                                                                                                                                         |                                                                                                                                         cAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTgaaa                                                                                                                                       >  1:12068/1‑140 (MQ=255)        ttCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGaaa                                                                                                                                >  1:37454/1‑140 (MQ=255)           gATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACaa                                                                                                                             <  1:554074/140‑1 (MQ=255)             tGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATg                                                                                                                           <  2:289514/140‑1 (MQ=255)                tttCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCg                                                                                                                        <  2:19224/140‑1 (MQ=255)                tttCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCg                                                                                                                        >  1:447083/1‑140 (MQ=255)                       ttttATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAAt                                                                                                                 <  2:906342/140‑1 (MQ=255)                           ataCTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGc                                                                                                             <  2:341473/140‑1 (MQ=255)                           ataCTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGc                                                                                                             >  1:998884/1‑140 (MQ=255)                           ataCTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGc                                                                                                             <  1:781552/140‑1 (MQ=255)                             aCTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTg                                                                                                           <  2:214423/140‑1 (MQ=255)                                aTTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAATATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGaaa                                                                                                        >  1:194396/1‑140 (MQ=255)                                aTTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGaga                                                                                                        >  2:1053178/1‑138 (MQ=255)                                 tttGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGagat                                                                                                       <  1:1053178/140‑4 (MQ=255)                                 tttGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAat                                                                                                       <  2:1011146/140‑1 (MQ=255)                                 tttGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAat                                                                                                       <  2:499768/140‑1 (MQ=255)                                   tGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAatat                                                                                                     <  2:566774/140‑1 (MQ=255)                                        gTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGtt                                                                                                <  2:998884/140‑1 (MQ=255)                                         tAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAg                                                                                                              >  1:883767/1‑125 (MQ=255)                                         tAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAg                                                                                                              <  2:883767/125‑1 (MQ=255)                                         tAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTa                                                                                               >  2:569870/1‑140 (MQ=255)                                           aTGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAgg                                                                                             >  2:211555/1‑140 (MQ=255)                                           aTGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAgg                                                                                             <  1:1042475/140‑1 (MQ=255)                                           aTGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGACACAATTAGCTGAAATATATGTTAgg                                                                                             <  2:990216/140‑1 (MQ=255)                                             gagaCAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTa                                                                                           <  1:75008/140‑1 (MQ=255)                                              agaCAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTAt                                                                                          >  1:202594/1‑140 (MQ=255)                                               gaCAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATc                                                                                         >  2:28122/1‑140 (MQ=255)                                                aCAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCg                                                                                        <  2:1079781/140‑1 (MQ=255)                                                aCAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCg                                                                                        <  1:860201/140‑1 (MQ=255)                                                  aGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAATATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCt                                                                                      <  2:194396/140‑1 (MQ=255)                                                   gCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACGATTAGCTGAAATATATGTTAGGTATCGCTa                                                                                     >  2:447301/1‑140 (MQ=255)                                                        ttACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCg                                                                                >  1:680248/1‑140 (MQ=255)                                                            ggTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACg                                                                            <  2:1041064/140‑1 (MQ=255)                                                              ttCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGa                                                                          <  1:415470/140‑1 (MQ=255)                                                                 tgatgaAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTg                                                                       >  1:502408/1‑140 (MQ=255)                                                                 tgatgaAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTg                                                                       <  1:126093/140‑1 (MQ=255)                                                                 tgatgaAAACATTATTTTTATGAGCCTTGTGGAAAAATTACTTTTGAATAAATACTCGATTAAAAATACGGTGAGAACTGAAACAATCGCGATACAATTAGTTGCGATATATGTTAGGTATCGCTATGGCGAACGGATTg                                                                       >  2:648316/1‑140 (MQ=255)                                                                   atgaAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCa                                                                     >  2:332838/1‑140 (MQ=255)                                                                   atgaAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCa                                                                     <  2:202594/140‑1 (MQ=255)                                                                        aaCATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCagaaga                                                                >  1:257514/1‑140 (MQ=255)                                                                        aaCATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCagaaga                                                                >  1:294282/1‑140 (MQ=255)                                                                          cATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCagaagaag                                                              >  2:43079/1‑140 (MQ=255)                                                                             tattTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGaaa                                                           >  1:59611/1‑140 (MQ=255)                                                                               tttttATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGaaaaa                                                         >  1:13378/1‑140 (MQ=255)                                                                                     tGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCatat                                                   <  2:680248/140‑1 (MQ=255)                                                                                      gAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATAtt                                                  <  2:139117/140‑1 (MQ=255)                                                                                            tgtgGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTa                                            <  2:502408/140‑1 (MQ=255)                                                                                             gtgGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTAc                                           <  1:702600/140‑1 (MQ=255)                                                                                               ggAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACgg                                         <  2:257514/140‑1 (MQ=255)                                                                                                 aaaaaTTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGaa                                       >  1:56257/1‑140 (MQ=255)                                                                                                   aaaTTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACt                                     >  1:714417/1‑140 (MQ=255)                                                                                                   aaaTTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACt                                     >  1:292234/1‑140 (MQ=255)                                                                                                   aaaTTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACt                                     >  1:434747/1‑140 (MQ=255)                                                                                                    aaTTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTa                                    >  2:183312/1‑140 (MQ=255)                                                                                                     aTTACATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTAc                                   <  1:28122/140‑1 (MQ=255)                                                                                                        aCATTTAGATAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAg                                >  1:788554/1‑140 (MQ=255)                                                                                                                aTAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTgg                        <  1:183312/140‑1 (MQ=255)                                                                                                                aTAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTgg                        <  2:294282/140‑1 (MQ=255)                                                                                                                 tAAATACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTggg                       <  2:714417/140‑1 (MQ=255)                                                                                                                    aTACTCTGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGgttg                    >  1:342400/1‑140 (MQ=255)                                                                                                                       ctctGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGttgttg                 <  2:13378/140‑1 (MQ=255)                                                                                                                          tGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGa                <  2:182581/138‑1 (MQ=255)                                                                                                                          tGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGa                >  1:182581/1‑138 (MQ=255)                                                                                                                          tGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAg               >  1:355364/1‑139 (MQ=255)                                                                                                                          tGTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAg               <  2:355364/139‑1 (MQ=255)                                                                                                                           gTTAAAAATACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAggg             <  2:292234/140‑1 (MQ=255)                                                                                                                              aaaaaTACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGc          >  1:214748/1‑140 (MQ=255)                                                                                                                                  aTACGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGCAAAg      <  2:59611/140‑1 (MQ=255)                                                                                                                                    aCGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGa            >  2:825944/1‑132 (MQ=255)                                                                                                                                    aCGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGa            <  1:825944/132‑1 (MQ=255)                                                                                                                                    aCGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGCAAAGtt    >  1:484347/1‑140 (MQ=255)                                                                                                                                    aCGGTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGCAAAGtt    >  1:552801/1‑140 (MQ=255)                                                                                                                                      ggTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGCa         <  1:81293/133‑1 (MQ=255)                                                                                                                                      ggTGAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGCAAAGTTAc  >  2:870123/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         CAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACATTTAGATAAATACTCTGTTAAAAATACGGTAAAAACTGAAACAATGGCGATACAATTAGCTGAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTAATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGCAAAGTTAC  >  NC_000913/527594‑527866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑