Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	699,536	T→C	intergenic (‑359/+38)	asnB ← / ← umpH	asparagine synthetase B/UMP phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	699,536	0	T	C	100.0%	136.9 / NA	39	intergenic (‑359/+38)	asnB/umpH	asparagine synthetase B/UMP phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/16);  total (23/16)

TGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACC  >  NC_000913/699400‑699670                                                                                                                                         |                                                                                                                                       tGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGccat                                                                                                                                     <  2:197612/140‑1 (MQ=255)  gCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGccatc                                                                                                                                    >  1:520139/1‑140 (MQ=255)          aGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGGCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGTGCATCAGGCAGTTTGGCGTTTGCCATCAGAGCCaa                                                                                                                            >  2:753156/1‑140 (MQ=255)            tGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGCCATCAGAGCCAAcc                                                                                                                          <  1:966806/140‑1 (MQ=255)              cGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGCCATCAGAGCCAACCAc                                                                                                                        <  2:951020/140‑1 (MQ=255)                               aTGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGCCATCAGAGCCAACCACGTCCGCAGACGTGGTTg                                                                                                       <  1:461126/140‑1 (MQ=255)                               aTGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGCCATCAGAGCCAACCACGTCCGCAGACGTGGTTg                                                                                                       >  2:461126/1‑140 (MQ=255)                               aTGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGCCATCAGAGCCAACCACGTCCGCAGACGTGGTTg                                                                                                       >  1:189720/1‑140 (MQ=255)                                              cTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGCCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGt                                                                                        >  2:1027959/1‑140 (MQ=255)                                                          aCAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGCCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGa                                                                            >  1:696579/1‑140 (MQ=255)                                                             aaaGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGCCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTg                                                                         <  1:1027959/140‑1 (MQ=255)                                                                 cATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGCCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACgg                                                                     >  2:532109/1‑140 (MQ=255)                                                                 cATGAAAATTCAATATATTACAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGCCATCAGAGCCAACCACGTCTGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGa                                                                        <  2:48313/137‑1 (MQ=255)                                                                 cATGAAAATTCAATATATTACAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGCCATCAGAGCCAACCACGTCTGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGa                                                                        >  1:48313/1‑137 (MQ=255)                                                                   tGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGCCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGt                                                                   <  1:753156/140‑1 (MQ=255)                                                                   tGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGCCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGt                                                                   <  1:819778/140‑1 (MQ=255)                                                                   tGAAAATTCAATATATTGCAGGAGCTGCGCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGCCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACATCGATTTCAGCGACTGACGGGt                                                                   >  1:773436/1‑140 (MQ=255)                                                                      aaaTTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGCCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTaaa                                                                >  2:56181/1‑140 (MQ=255)                                                                      aaaTTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGCCATCAGAGCCAACCACGGCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTaaa                                                                <  2:773436/140‑1 (MQ=255)                                                                                tatTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGCCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTggg                                                      >  2:881264/1‑140 (MQ=255)                                                                                   tGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGCCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTgggg                                                     >  1:384967/1‑138 (MQ=255)                                                                                   tGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGCCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTgggg                                                     >  1:408106/1‑138 (MQ=255)                                                                                   tGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGCCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTgggg                                                     <  2:408106/138‑1 (MQ=255)                                                                                   tGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGCCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTgggg                                                     <  2:384967/138‑1 (MQ=255)                                                                                   tGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGCCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCg                                                   <  1:476646/140‑1 (MQ=255)                                                                                   tGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGCCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCg                                                   <  2:696579/140‑1 (MQ=255)                                                                                   tGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGCCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCg                                                   >  2:476646/1‑140 (MQ=255)                                                                                     cAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGCCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGa                                                 >  1:549144/1‑140 (MQ=255)                                                                                          gCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGCCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGc                                            <  2:328008/140‑1 (MQ=255)                                                                                          gCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGCCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGc                                            >  1:328008/1‑140 (MQ=255)                                                                                                        aTAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGCCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATAtc                              >  1:546599/1‑140 (MQ=255)                                                                                                        aTAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGCCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATAtc                              >  2:490630/1‑140 (MQ=255)                                                                                                        aTAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGCCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATAtc                              >  1:763235/1‑140 (MQ=255)                                                                                                            gCGTAGCGCATCAGGCAGTTTGGCGTTTGCCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATAtcgtcg                          <  2:487490/140‑1 (MQ=255)                                                                                                            gCGTAGCGCATCAGGCAGTTTGGCGTTTGCCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATAtcgtcg                          <  2:546599/140‑1 (MQ=255)                                                                                                             cGTAGCGCATCAGGCAGTTTGGCGTTTGCCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGa                         >  1:781765/1‑140 (MQ=255)                                                                                                                    cATCAGGCAGTTTGGCGTTTGCCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGcgacga                  <  1:490630/140‑1 (MQ=255)                                                                                                                      tCAGGCAGTTTGGCGTTTGCCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGaaa                <  2:763235/140‑1 (MQ=255)                                                                                                                      tCAGGCAGTTTGGCGTTTGCCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGaaa                >  2:780457/1‑140 (MQ=255)                                                                                                                              tttGGCGTTTGCCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGaa        >  1:516917/1‑140 (MQ=255)                                                                                                                              tttGGCGTTTGCCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGaa        >  1:1001861/1‑140 (MQ=255)                                                                                                                                    gTTTGCCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCAcc  >  1:716847/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       TGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACC  >  NC_000913/699400‑699670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑