Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	768,190	C→T	D71D (GAC→GAT)	mngB →	alpha‑mannosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	768,190	0	C	T	100.0%	223.0 / NA	64	D71D (GAC→GAT)	mngB	alpha‑mannosidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/32);  total (32/32)

ACGTATTCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCATCGTCCGTAATCTGATGTACGGAATGCGTGACTGCCT  >  NC_000913/768055‑768315                                                                                                                                        |                                                                                                                              aCGTATTCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCg                                                                                                                             >  1:573515/1‑138 (MQ=255)  cGTATTCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCgtgt                                                                                                                          >  2:57020/1‑140 (MQ=255)  cGTATTCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCgtgt                                                                                                                          >  1:373059/1‑140 (MQ=255)      ttCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTgaag                                                                                                                      <  2:373059/140‑1 (MQ=255)       tCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTgaaga                                                                                                                     <  1:149136/140‑1 (MQ=255)         tgctgGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGaaa                                                                                                                   <  1:105536/140‑1 (MQ=255)         tgctgGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGaaa                                                                                                                   >  1:304890/1‑140 (MQ=255)           ctgGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCgtgt                                                                                                                          >  2:767267/1‑131 (MQ=255)           ctgGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCgtgt                                                                                                                          <  1:767267/131‑1 (MQ=255)           ctgGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCgt                                                                                                                            <  2:930331/129‑1 (MQ=255)           ctgGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCgt                                                                                                                            >  1:930331/1‑129 (MQ=255)           ctgGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACa                                                                                                                 >  1:188038/1‑140 (MQ=255)           ctgGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACa                                                                                                                 >  1:715156/1‑140 (MQ=255)            tgGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAg                                                                                                                >  1:155188/1‑140 (MQ=255)              gTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGt                                                                                                              >  2:453751/1‑140 (MQ=255)                       atGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCgg                                                                                                     <  1:833529/140‑1 (MQ=255)                        tGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGc                                                                                                    >  1:422413/1‑140 (MQ=255)                             gagaTCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGtt                                                                                               >  1:867835/1‑140 (MQ=255)                             gagaTCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGtt                                                                                               >  1:825854/1‑140 (MQ=255)                             gagaTCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGtt                                                                                               >  1:876517/1‑140 (MQ=255)                             gagaTCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGtt                                                                                               >  2:232090/1‑140 (MQ=255)                                aTCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGAt                                                                                            >  2:175121/1‑140 (MQ=255)                                aTCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGAt                                                                                            >  1:770466/1‑140 (MQ=255)                                   cTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTAt                                                                                         <  2:876517/140‑1 (MQ=255)                                     gtgCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCg                                                                                       >  2:152328/1‑140 (MQ=255)                                     gtgCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCg                                                                                       <  1:447501/140‑1 (MQ=255)                                       gCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGc                                                                                     <  2:770466/140‑1 (MQ=255)                                        ccGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGcc                                                                                    >  2:738081/1‑140 (MQ=255)                                         cGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGccc                                                                                   <  2:809165/140‑1 (MQ=255)                                         cGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGccc                                                                                   <  2:867835/140‑1 (MQ=255)                                         cGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGccc                                                                                   <  1:738081/140‑1 (MQ=255)                                         cGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGccc                                                                                   <  2:715156/140‑1 (MQ=255)                                         cGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGccc                                                                                   <  1:453751/140‑1 (MQ=255)                                         cGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGccc                                                                                   <  2:825854/140‑1 (MQ=255)                                           aCTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCt                                                                                 >  2:41771/1‑140 (MQ=255)                                              ggAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGt                                                                              <  2:304890/140‑1 (MQ=255)                                               gAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGta                                                                             <  2:155188/140‑1 (MQ=255)                                                   aGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATAcc                                                                         <  2:188038/140‑1 (MQ=255)                                                     gACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGccc                                                                                   <  1:82070/128‑1 (MQ=255)                                                     gACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGccc                                                                                   >  2:377173/1‑128 (MQ=255)                                                     gACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGccc                                                                                   <  1:350753/128‑1 (MQ=255)                                                     gACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGccc                                                                                   >  2:350753/1‑128 (MQ=255)                                                     gACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGccc                                                                                   >  2:82070/1‑128 (MQ=255)                                                     gACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGccc                                                                                   <  1:377173/128‑1 (MQ=255)                                                        aaCGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGAc                                                                    <  1:152328/140‑1 (MQ=255)                                                          cGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGta                                                                             >  1:574667/1‑129 (MQ=255)                                                          cGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGta                                                                             <  2:574667/129‑1 (MQ=255)                                                             aTACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATa                                                               >  2:811190/1‑140 (MQ=255)                                                             aTACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATa                                                               >  2:351370/1‑140 (MQ=255)                                                               aCAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATAcc                                                             >  1:295616/1‑140 (MQ=255)                                                               aCAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATAcc                                                             <  2:381272/140‑1 (MQ=255)                                                                   atatTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGa                                                         >  2:806789/1‑140 (MQ=255)                                                                         cGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGt                                                     >  2:678649/1‑138 (MQ=255)                                                                         cGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGt                                                     <  1:678649/138‑1 (MQ=255)                                                                           tACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCt                                                 >  2:847141/1‑140 (MQ=255)                                                                               cGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCgg                                             <  1:175121/140‑1 (MQ=255)                                                                               cGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCgg                                             <  2:258956/140‑1 (MQ=255)                                                                                  cGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAAt                                          <  1:806789/140‑1 (MQ=255)                                                                                   gggCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATc                                         >  2:235702/1‑140 (MQ=255)                                                                                                   tCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCATCGTCCGTAATCTg                         <  1:1067435/140‑1 (MQ=255)                                                                                                            attTCGCGGTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCATCGTCCGTAATCTGATGTACGGa                >  1:139943/1‑140 (MQ=255)                                                                                                                   ggTGAAACCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCATCGTCCGTAATCTGATGTACGGAATGCGTg         <  2:139943/140‑1 (MQ=255)                                                                                                                        aaCCGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCATCGTCCGTAATCTGATGTACGGAATGCGTGACTGc    <  1:847141/140‑1 (MQ=255)                                                                                                                          ccGGAAAACAAAGATCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCATCGTCCGTAATCTGATGTACGGAATGCGTGACTGCCt  <  2:295616/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                              ACGTATTCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCCATCGTCCGTAATCTGATGTACGGAATGCGTGACTGCCT  >  NC_000913/768055‑768315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑