Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	778,662	A→G	Q308R (CAG→CGG)	tolB →	Tol‑Pal system periplasmic protein TolB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	778,662	0	A	G	100.0%	247.4 / NA	68	Q308R (CAG→CGG)	tolB	Tol‑Pal system periplasmic protein TolB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/32);  total (36/32)

TCGAAAACCGGTAGTCTGAACCTGTACGTAATGGATTTGGCTTCTGGTCAGATCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCAGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCGGATGTCAGCAGCGACGGTAAATTTATGGTAATGGTCAGCTCCAAT  >  NC_000913/778526‑778795                                                                                                                                         |                                                                                                                                      tCGAAAACCGGTAGTCTGAACCTGTACGTAATGGATTTGGCTTCTGGTCAGATCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGc                                                                                                                                    <  1:41506/140‑1 (MQ=255)           gTAGTCTGAACCTGTACGTAATGGATTTGGCTTCTGGTCAGATCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCg                                                                                                                          <  2:10073/140‑1 (MQ=255)           gTAGTCTGAACCTGTACGTAATGGATTTGGCTTCTGGTCAGATCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCg                                                                                                                          <  2:972805/140‑1 (MQ=255)           gTAGTCTGAACCTGTACGTAATGGATTTGGCTTCTGGTCAGATCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCg                                                                                                                          <  1:344434/140‑1 (MQ=255)                 tGAACCTGTACGTAATGGATTTGGCTTCTGGTCAGATCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGtt                                                                                                                    >  1:918343/1‑140 (MQ=255)                  gAACCTGTACGTAATGGATTTGGCTTCTGGTCAGATCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGttt                                                                                                                   >  1:603201/1‑140 (MQ=255)                   aaCCTGTACGTAATGGATTTGGCTTCTGGTCAGATCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTta                                                                                                                  >  2:704067/1‑140 (MQ=255)                     ccTGTACGTAATGGATTTGGCTTCTGGTCAGATCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTtata                                                                                                                <  1:252532/140‑1 (MQ=255)                        gTACGTAATGGATTTGGCTTCTGGTCAGATCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTcc                                                                                                                           >  2:479472/1‑126 (MQ=255)                        gTACGTAATGGATTTGGCTTCTGGTCAGATCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTcc                                                                                                                           <  1:479472/126‑1 (MQ=255)                             tAATGGATTTGGCTTCTGGTCAGATCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAat                                                                                                        >  1:913560/1‑140 (MQ=255)                                tGGATTTGGCTTCTGGTCAGATCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATc                                                                                                     >  2:196000/1‑140 (MQ=255)                                tGGATTTGGCTTCTGGTCAGATCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATc                                                                                                     >  1:77805/1‑140 (MQ=255)                                   aTTTGGCTTCTGGTCAGATCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAAc                                                                                                  >  2:585559/1‑140 (MQ=255)                                   aTTTGGCTTCTGGTCAGATCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAAc                                                                                                  >  2:200927/1‑140 (MQ=255)                                   aTTTGGCTTCTGGTCAGATCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAAc                                                                                                  >  1:628982/1‑140 (MQ=255)                                     ttGGCTTCTGGTCAGATCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTtata                                                                                                                <  2:154756/124‑1 (MQ=255)                                     ttGGCTTCTGGTCAGATCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTtata                                                                                                                >  1:154756/1‑124 (MQ=255)                                     ttGGCTTCTGGTCAGATCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAAcgg                                                                                                >  2:678060/1‑140 (MQ=255)                                     ttGGCTTCTGGTCAGATCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAAcgg                                                                                                >  1:791117/1‑140 (MQ=255)                                       ggCTTCTGGTCAGATCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAAcggcg                                                                                              <  2:143898/140‑1 (MQ=255)                                          ttCTGGTCAGATCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTg                                                                                           <  2:546796/140‑1 (MQ=255)                                               gTCAGATCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCca                                                                                      <  2:791117/140‑1 (MQ=255)                                                tCAGATCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCcac                                                                                     <  2:568396/140‑1 (MQ=255)                                                tCAGATCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCcac                                                                                     <  1:497652/140‑1 (MQ=255)                                                tCAGATCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCcac                                                                                     <  1:548392/140‑1 (MQ=255)                                                  aGATCCGCTAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCcac                                                                                     <  2:672828/138‑1 (MQ=255)                                                  aGATCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCcac                                                                                     >  1:672828/1‑138 (MQ=255)                                                  aGATCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACaa                                                                                   >  1:641671/1‑140 (MQ=255)                                                    aTCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACg                                                                                 >  1:925818/1‑140 (MQ=255)                                                      ccGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTa                                                                               <  1:678060/140‑1 (MQ=255)                                                         ccAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGt                                                                                >  1:246047/1‑136 (MQ=255)                                                         ccAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGt                                                                                <  2:246047/136‑1 (MQ=255)                                                         ccAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTa                                                                            >  1:776922/1‑140 (MQ=255)                                                            ggTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCcaca                                                                                    <  1:50371/129‑1 (MQ=255)                                                            ggTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCcaca                                                                                    >  2:50371/1‑129 (MQ=255)                                                              tGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTgg                                                                       >  1:726487/1‑140 (MQ=255)                                                              tGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTgg                                                                       >  1:382483/1‑140 (MQ=255)                                                                       gTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAGGTTCg                                                              <  2:913560/140‑1 (MQ=255)                                                                       gTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAGGTTCg                                                              <  2:908658/140‑1 (MQ=255)                                                                        tCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAGGTTCGc                                                             >  2:345928/1‑140 (MQ=255)                                                                        tCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAGGTTCGc                                                             <  1:345928/140‑1 (MQ=255)                                                                                 cAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAGGTTCGCAGAACCAgg                                                    <  1:200927/140‑1 (MQ=255)                                                                                    tACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCA‑TTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAgg                                                                  >  1:94728/1‑122 (MQ=255)                                                                                    tACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCA‑TTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAgg                                                                  <  2:94728/122‑1 (MQ=255)                                                                                      ccgaaccgaCCTGGTTCCCTGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCg                                               >  1:309093/1‑140 (MQ=255)                                                                                      ccgaaccgaCCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCg                                               <  2:726487/140‑1 (MQ=255)                                                                                      ccgaaccgaCCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCg                                               <  2:382483/140‑1 (MQ=255)                                                                                      ccgaaccgaCCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCg                                               >  1:677990/1‑140 (MQ=255)                                                                                        gaaccgaCCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAGGTTTGCAGAACCCGGATGCGGa                                             >  1:271004/1‑140 (MQ=255)                                                                                         aaccgaCCTGGTT‑CCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCGGATg                                           >  2:803778/1‑140 (MQ=255)                                                                                          accgaCCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCGGATg                                           >  2:76634/1‑140 (MQ=255)                                                                                                  ggTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCGGATGTCagcagc                                   <  2:641671/140‑1 (MQ=255)                                                                                                  ggTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCGGATGTCagcagc                                   <  2:242447/140‑1 (MQ=255)                                                                                                        cGGATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCGGATGTCAGCAGCGACGGt                             <  1:585559/140‑1 (MQ=255)                                                                                                         ggATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCGGATGTCAGCAGCGACGGTa                            >  1:1019265/1‑140 (MQ=255)                                                                                                         ggATAGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGACGGTGCGCCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCGGATGTCAGCAGCGACGGTa                            >  2:762057/1‑140 (MQ=255)                                                                                                             aGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCGGATGTCAGCAGCGACGGTAAAtt                        >  1:417047/1‑140 (MQ=255)                                                                                                             aGCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCGGATGTCAGCAGCGACGGTAAAtt                        <  2:918343/140‑1 (MQ=255)                                                                                                              gCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCGGATGTcagcag                                    <  1:558825/127‑1 (MQ=255)                                                                                                              gCCAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCGGATGTcagcag                                    >  2:558825/1‑127 (MQ=255)                                                                                                               ccAGAACCTGGCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCGGATGTCAGCAGCGACGGTAAATTTa                      <  2:776922/140‑1 (MQ=255)                                                                                                                        ggCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCGGATGTCAGCAGCGACGGTAAATTTATGGTa                 <  2:358033/136‑1 (MQ=255)                                                                                                                        ggCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCGGATGTCAGCAGCGACGGTAAATTTATGGTa                 >  1:358033/1‑136 (MQ=255)                                                                                                                         gCATTTACTTCTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCGGATGTCAGCAGCGACGGTAAATTTATGGTAATGGt            >  2:868956/1‑140 (MQ=255)                                                                                                                              tACTTCTGACCGGGCCGGTCGTCCACAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCGGATGTCAGCAGCGACGGTAAATTTATGGTAATGGTc           <  2:666171/136‑1 (MQ=255)                                                                                                                              tACTTCTGACCGGGCCGGTCGTCCACAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCGGATGTCAGCAGCGACGGTAAATTTATGGTAATGGTc           >  1:666171/1‑136 (MQ=255)                                                                                                                                   cTGACCGGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCGGATGTCAGCAGCGACGGTAAATTTATGGTAATGGTCAGCTCCAAt  >  2:538474/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      TCGAAAACCGGTAGTCTGAACCTGTACGTAATGGATTTGGCTTCTGGTCAGATCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCAGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCGGATGTCAGCAGCGACGGTAAATTTATGGTAATGGTCAGCTCCAAT  >  NC_000913/778526‑778795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑