Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 878,840 A→G K33K (AAA→AAG)  yliI → aldose sugar dehydrogenase YliI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913878,8400AG96.5% 190.2 / ‑3.6 57K33K (AAA→AAG) yliIaldose sugar dehydrogenase YliI
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (29/26);  total (30/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGACTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGG  >  NC_000913/878704‑878976
                                                                                                                                        |                                                                                                                                        
cTGACTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTc                                                                                                                                       >  2:208851/1‑140 (MQ=255)
   aCTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGAc                                                                                                                                    <  2:405303/140‑1 (MQ=255)
   aCTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGAc                                                                                                                                    >  1:94971/1‑140 (MQ=255)
   aCTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGAc                                                                                                                                    >  2:1061175/1‑140 (MQ=255)
   aCTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGAc                                                                                                                                    <  1:347574/140‑1 (MQ=255)
   aCTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGAc                                                                                                                                    <  2:94971/140‑1 (MQ=255)
   aCTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGAc                                                                                                                                    <  2:891964/140‑1 (MQ=255)
   aCTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGAc                                                                                                                                    >  2:213261/1‑140 (MQ=255)
     tACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGACCa                                                                                                                                  <  1:208851/140‑1 (MQ=255)
       cGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGAc                                                                                                                                    <  1:215989/136‑1 (MQ=255)
       cGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGAc                                                                                                                                    >  2:215989/1‑136 (MQ=255)
       cGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATACATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGaa                                                                                                                                    >  2:220558/1‑135 (MQ=255)
       cGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATACATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGaa                                                                                                                                    <  1:220558/136‑2 (MQ=255)
        gCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGACCATcc                                                                                                                               >  2:530118/1‑140 (MQ=255)
             aaGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGAc                                                                                                                                    >  1:258388/1‑130 (MQ=255)
             aaGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGAc                                                                                                                                    <  2:258388/130‑1 (MQ=255)
                  agagTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGACCATCCCTGGGCACTg                                                                                                                     >  2:74779/1‑140 (MQ=255)
                    agTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGACCATCCCTGGGCACTGGc                                                                                                                   >  2:477964/1‑140 (MQ=255)
                    agTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCATTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGACCa                                                                                                                                  <  1:167603/125‑1 (MQ=255)
                    agTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCATTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGACCa                                                                                                                                  >  2:167603/1‑125 (MQ=255)
                        aaTCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGACCATCCCTGGGCACTGGCCttt                                                                                                               <  2:376767/140‑1 (MQ=255)
                              gAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGACCATCCCTGGGCACTGGCCTTTTTAccc                                                                                                         <  1:477964/140‑1 (MQ=255)
                               aGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGACCATCCCTGGGCACTGGCCTTTTTACCCg                                                                                                        <  1:1061175/140‑1 (MQ=255)
                               aGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGACCATCCCTGGGCACTGGCCTTTTTACCCg                                                                                                        >  2:6963/1‑140 (MQ=255)
                               aGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGACCATCCCTGGGCACTGGCCTTTTTAcc                                                                                                          >  1:265557/1‑138 (MQ=255)
                               aGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGACCATCCCTGGGCACTGGCCTTTTTAcc                                                                                                          <  2:265557/138‑1 (MQ=255)
                                ggCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGACCATCCCTGGGCACTGGCCTTTTTACCCGa                                                                                                       >  1:532344/1‑140 (MQ=255)
                                    ttATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAAt                                                                                                   >  2:313762/1‑140 (MQ=255)
                                    ttATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAAt                                                                                                   <  1:561233/140‑1 (MQ=255)
                                    ttATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAAt                                                                                                   <  1:1039617/140‑1 (MQ=255)
                                      aTGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCa                                                                                                 <  1:6963/140‑1 (MQ=255)
                                        gCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACg                                                                                               <  1:313762/140‑1 (MQ=255)
                                          aTCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCCGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGt                                                                                             <  1:18052/140‑1 (MQ=255)
                                            cGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTAt                                                                                           >  1:279999/1‑140 (MQ=255)
                                              aCAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTAt                                                                                           <  1:352803/138‑1 (MQ=255)
                                              aCAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTAt                                                                                           >  2:352803/1‑138 (MQ=255)
                                              aCAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTATGt                                                                                         >  1:1062764/1‑140 (MQ=255)
                                                           cTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTgtg                                                                            <  1:74779/140‑3 (MQ=255)
                                                              gtgCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCgcgg                                                                         >  1:280803/1‑140 (MQ=255)
                                                                 ccccTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCgcggcgg                                                                      >  1:493269/1‑140 (MQ=255)
                                                                    cTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGa                                                                   >  1:562510/1‑140 (MQ=255)
                                                                             ctttcttCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCa                                                          >  1:823921/1‑140 (MQ=255)
                                                                              tttcttCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCAc                                                         >  2:247112/1‑140 (MQ=255)
                                                                                   tcCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCa                                                    <  2:820220/140‑1 (MQ=255)
                                                                                      gCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGc                                                 >  2:387901/1‑140 (MQ=255)
                                                                                      gCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGc                                                 <  1:387901/140‑1 (MQ=255)
                                                                                            tGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGaaa                                           >  1:1037604/1‑140 (MQ=255)
                                                                                            tGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGaaa                                           <  2:532344/140‑1 (MQ=255)
                                                                                                    tCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTAt                                   >  1:249926/1‑140 (MQ=255)
                                                                                                         cAACGGTAAATGTCGAAGTACTGCAAGACAAGCTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTgcg                              >  1:780983/1‑140 (MQ=255)
                                                                                                            cGGTAAATGTCGAAGTACTGCAAGACAAGCTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGcgccg                           <  2:1062764/140‑1 (MQ=255)
                                                                                                             ggTAAATGTCGAAGTACTGCAAGACAAGCTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTgcg                              >  2:335804/1‑136 (MQ=255)
                                                                                                             ggTAAATGTCGAAGTACTGCAAGACAAGCTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTgcg                              <  1:335804/136‑1 (MQ=255)
                                                                                                                   tGTCGAAGTACTGCAAGACAAGCTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCg                    <  1:619684/140‑1 (MQ=255)
                                                                                                                              tGCAAGACAAGCTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGAc         >  2:970066/1‑140 (MQ=255)
                                                                                                                               gCAAGACAAGCTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACg        >  1:893140/1‑140 (MQ=255)
                                                                                                                                     cAAGCTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTggg  <  2:893140/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
CTGACTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTCTGGGCGGCTCCTGCAACGGTAAATGTCGAAGTACTGCAAGACAAACTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGG  >  NC_000913/878704‑878976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑