Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	48,192	A→G	T142A (ACG→GCG)	kefC →	K(+) : H(+) antiporter KefC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	48,192	0	A	G	100.0%	114.2 / NA	33	T142A (ACG→GCG)	kefC	K(+) : H(+) antiporter KefC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/19);  total (14/19)

GCGGCGGCCTGCTGGGGCTGTTCTGCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGACGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGATATCGCGGCGATCCCGCTGGTGGCGATGATTCCGCTACTGGCAACGAGCAGTGCCTCGACGACGATGGGCGCATTTGCTCTCTCGGCGTTAA  >  NC_000913/48058‑48327                                                                                                                                       |                                                                                                                                        gcggcgGCCTGCTGGGGCTGTTCTGCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGGCGCaa                                                                                                                                    <  1:998001/140‑1 (MQ=255) gcggcgGCCTGCTGGGGCTGTTCTGCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGGCGCaa                                                                                                                                    <  1:717106/140‑1 (MQ=255) gcggcgGCCTCCTGCGGCTGTTCTGCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGGCGCaa                                                                                                                                    <  2:673235/140‑1 (MQ=255)              ggggCTGTTCTGCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGGCGCAAATGGGTCGCAGTg                                                                                                                       <  1:330539/140‑1 (MQ=255)                  cTGTTCTGCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGGCGCAAATGGGTCGCAGTGCCtt                                                                                                                   <  2:173066/140‑1 (MQ=255)                     ttCTGCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGGCGCAAATGGGTCGCAGTGCCTTTGc                                                                                                                >  1:287627/1‑140 (MQ=255)                     ttCTGCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGGCGCAAATGGGTCGCAGTGCCTTTGc                                                                                                                >  1:8201/1‑140 (MQ=255)                        tGCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGGCGCAAATGGGTCGCAGTGCCTTTGCGGt                                                                                                             <  2:437286/140‑1 (MQ=255)                            tGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGGCGCAAATGGGTCGCAGTGCCTTTGCGGtgctg                                                                                                         <  1:684020/140‑1 (MQ=255)                                 cTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGGCGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGtt                                                                                                    <  2:287627/140‑1 (MQ=255)                                      gCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGGCGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAgg                                                                                               >  2:1093284/1‑140 (MQ=255)                                       cTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGGCGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGa                                                                                              <  1:1093284/140‑1 (MQ=255)                                          cgcTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGGCGCAAATGGGTCGCAGTGCCTTTGCGGt                                                                                                             <  1:547497/122‑1 (MQ=255)                                          cgcTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGGCGCAAATGGGTCGCAGTGCCTTTGCGGt                                                                                                             >  2:547497/1‑122 (MQ=255)                                          cgcTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGGCGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGatat                                                                                           <  2:749753/140‑1 (MQ=255)                                           gcTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGGCGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGATATc                                                                                          >  2:71045/1‑140 (MQ=255)                                                                  ggCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGGCGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGATATCGCGGCGATCCCGCTGGTGGCgat                                                                   <  2:185921/140‑1 (MQ=255)                                                                              gcgcTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGGCGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGATATCGCGGCGATCCCGCTGGTGGc                                                                      >  1:452001/1‑125 (MQ=255)                                                                              gcgcTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGGCGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGATATCGCGGCGATCCCGCTGGTGGc                                                                      <  2:452001/125‑1 (MQ=255)                                                                                      ctctACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGGCGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGATATCGCGGCGATCCCGCTGGTGGCGATGATTCCGCTACTGGCa                                                   >  1:524448/1‑136 (MQ=255)                                                                                      ctctACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGGCGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGATATCGCGGCGATCCCGCTGGTGGCGATGATTCCGCTACTGGCa                                                   <  2:524448/136‑1 (MQ=255)                                                                                          aCGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGGCGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGATATCGCGGCGATCCCGCTGGTGGCGATGATTCCGCTACTGGCAACGAGCAg                                           <  1:71045/140‑1 (MQ=255)                                                                                             gCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGGCGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGATATCGCGGCGATCCCGCTGGTGGCGATGATTCCGCTACTGGCAACGAGCAGTGc                                        <  2:74112/140‑1 (MQ=255)                                                                                              cGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGGCGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGATATCGCGGCGATCCCGCTGGTGGCGATGATTCCGCTACTGGCAACGAGCAGTGcc                                       >  1:325705/1‑140 (MQ=255)                                                                                                aTTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGGCGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGATATCGCGGCGATCCCGCTGGTGGCGATGATTCCGCTACTGGCa                                                   >  2:653949/1‑126 (MQ=255)                                                                                                aTTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGGCGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGATATCGCGGCGATCCCGCTGGTGGCGATGATTCCGCTACTGGCa                                                   <  1:653949/126‑1 (MQ=255)                                                                                                aTTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGGCGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGATATCGCGGCGATCCCGCTGGTGGCGATGATTCCGCTACTGGCAACGAGCAGTGCCTc                                     >  1:876466/1‑140 (MQ=255)                                                                                                 ttGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGGCGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCT‑‑‑‑‑‑GGATATCGCGGCGATCCCGCTGGTGGCGATGATTCCGCTACTGGCAACGAGCAGTGCCTcgacgacg                              >  1:587030/1‑140 (MQ=255)                                                                                                                      aaCGCAATCTGATGGTGGCGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGATATCGCGGCGATCCCGCTGGTGGCGATGATTCCGCTACTGGCAACGAGCAGTGCCTCGACGACGATGGGCGCATTTGct               >  2:577874/1‑140 (MQ=255)                                                                                                                           aaTCTGATGGTGGCGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGATATCGCGGCGATCCCGCTGGTGGCGATGATTCCGCTACTGGCAACGAGCAGTGCCTCGACGACGATGGGCGCATTTGCtctctc          <  2:8201/140‑1 (MQ=255)                                                                                                                               tGATGGTGGCGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGATATCGCGGCGATCCCGCTGGTGGCGATGATTCCGCTACTGGCAACGAGCAGTGCCTCGACGACGATGGGCGCATTTGCTCTCTCGGCg      <  1:469495/140‑1 (MQ=255)                                                                                                                                 aTGGTGGCGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGATATCGCGGCGATCCCGCTGGTGGCGATGATTCCGCTACTGGCAACGAGCAGTGCCTCGACGACGATGGGCGCATTTGCTCTCTCGGCGtt    >  1:299252/1‑140 (MQ=255)                                                                                                                                   ggTGGCGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGATATCGCGGCGATCCCGCTGGTGGCGATGATTCCGCTACTGGCAACGAGCAGTGCCTCGACGACGATGGGCGCATTTGCTCTCTCGGCGTTaa  >  2:104243/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        GCGGCGGCCTGCTGGGGCTGTTCTGCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGACGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGATATCGCGGCGATCCCGCTGGTGGCGATGATTCCGCTACTGGCAACGAGCAGTGCCTCGACGACGATGGGCGCATTTGCTCTCTCGGCGTTAA  >  NC_000913/48058‑48327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑