Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	945,259	A→G	V100A (GTC→GCC)	ycaC ←	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	945,259	0	A	G	100.0%	199.0 / NA	55	V100A (GTC→GCC)	ycaC	putative hydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/26);  total (29/26)

TTTCATTAAAGGTCCCTGACGCGTCAGTCACGACAAAGACGTCAAACCCTTCTTCAATGGCTGAAAGCGCCGGGAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGACAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGCGAGCAATGTAAGGTGTATCGGGGAATTGTGCTTTCAGTTCTGGAACTAATGGGCCGTTAGGACCGGTTTCAAAACTGGTGGTAAGAA  >  NC_000913/945122‑945394                                                                                                                                          |                                                                                                                                        tttCATTAAAGGTCCCTGACGCGTCAGTCACGACAAAGACGTCAAACCCTTCTTCAATGGCTGAAAGCGCCGGGAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCa                                                                                                                                       >  2:525962/1‑140 (MQ=255)  ttCATTAAAGGTCCCTGACGCGTCAGTCACGACAAAGACGTCAAACCCTTCTTCAATGGCTGAAAGCGCCGGGAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAg                                                                                                                                      <  2:314181/140‑1 (MQ=255)              cccTGACGCGTCAGTCACGACAAAGACGTCAAACCCTTCTTCAATGGCTGAAAGCGCCGGGAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAAt                                                                                                                          >  2:472050/1‑140 (MQ=255)              cccTGACGCGTCAGTCACGACAAAGACGTCAAACCCTTCTTCAATGGCTGAAAGCGCCGGGAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAAt                                                                                                                          <  2:124328/140‑1 (MQ=255)               ccTGACGCGTCAGTCACGACAAAGACGTCAAACCCTTCTTCAATGGCTGAAAGCGCCGGGAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATc                                                                                                                         >  2:502761/1‑140 (MQ=255)                 tGACGCGTCAGTCACGACAAAGACGTCAAACCCTTCTTCAATGGCTGAAAGCGCCGGGAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCtt                                                                                                                       >  1:167429/1‑140 (MQ=255)                   aCGCGTCAGTCACGACAAAGACGTCAAACCCTTCTTCAATGGCTGAAAGCGCCGGGAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCg                                                                                                                     <  1:525962/140‑1 (MQ=255)                      cgTCAGTCACGACAAAGACGTCAAACCCTTCTTCAATGGCTGAAAGCGCCGGGAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTa                                                                                                                  >  1:126288/1‑140 (MQ=255)                      cgTCAGTCACGACAAAGACGTCAAACCCTTCTTCAATGGCTGAAAGCGCCGGGAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTa                                                                                                                  >  1:14112/1‑140 (MQ=255)                      cgTCAGTCACGACAAAGACGTCAAACCCTTCTTCAATGGCTGAAAGCGCCGGGAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTa                                                                                                                  >  1:465680/1‑140 (MQ=255)                         cagtcaCGACAAAGACGTCAAACCCTTCTTCAATGGCTGAAAGCGCCGGGAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATcc                                                                                                               <  1:424012/140‑1 (MQ=255)                               cGACAAAGACGTCAAACCCTTCTTCAATGGCTGAAAGCGCCGGGAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCg                                                                                                         >  2:957125/1‑140 (MQ=255)                                 aCAAAGACGTCAAACCCTTCTTCAATGGCTGAAAGCGCCGGGAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGtt                                                                                                       >  1:416167/1‑140 (MQ=255)                                     aGACGTCAAACCCTTCTTCAATGGCTGAAAGCGCCGGGAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAata                                                                                                   >  2:417338/1‑140 (MQ=255)                                     aGACGTCAAACCCTTCTTCAATGGCTGAAAGCGCCGGGAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAata                                                                                                   >  2:540504/1‑140 (MQ=255)                                     aGACGTCAAACCCTTCTTCAATGGCTGAAAGCGCCGGGAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAata                                                                                                   >  1:263210/1‑140 (MQ=255)                                        cGTCAAACCCTTCTTCAATGGCTGAAAGCGCCGGGAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATAttt                                                                                                >  1:38331/1‑140 (MQ=255)                                              aCCCTTCTTCAATGGCTGAAAGCGCCGGGAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTCTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCggg                                                                                          >  2:279310/1‑140 (MQ=255)                                                 cttcttCAATGGCTGAAAGCGCCGGGAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGCGa                                                                                       <  2:1046672/140‑1 (MQ=255)                                                 cttcttCAATGGCTGAAAGCGCCGGGAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGCGa                                                                                       <  1:417338/140‑1 (MQ=255)                                                  ttcttcAATGGCTGAAAGCGCCGGGAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGCGAg                                                                                      <  2:123405/140‑1 (MQ=255)                                                  ttcttcAATGGCTGAAAGCGCCGGGAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGCGAg                                                                                      <  1:957125/140‑1 (MQ=255)                                                  ttcttcAATGGCTGAAAGCGCCGGGAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGCGAg                                                                                      <  1:721680/140‑1 (MQ=255)                                                      tcAATGGCTGAAAGCGCCGGGAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGCGAGCAAt                                                                                  <  1:540504/140‑1 (MQ=255)                                                      tcAATGGCTGAAAGCGCCGGGAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGCGAGCAAt                                                                                  <  1:502761/140‑1 (MQ=255)                                                        aaTGGCTGAAAGCGCCGGGAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGc                                                                                         <  1:160713/131‑1 (MQ=255)                                                        aaTGGCTGAAAGCGCCGGGAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGc                                                                                         >  2:160713/1‑131 (MQ=255)                                                           ggCTGAAAGCGCCGGGAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTcc                                                                                              >  2:187769/1‑123 (MQ=255)                                                           ggCTGAAAGCGCCGGGAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTcc                                                                                              <  1:187769/123‑1 (MQ=255)                                                            gCTGAAAGCGCCGGGAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGCGAGCAATGTAAgg                                                                            >  1:667223/1‑140 (MQ=255)                                                            gCTGAAAGCGCCGGGAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGCGAGCAATGTAAgg                                                                            <  2:465680/140‑1 (MQ=255)                                                             cTGAAAGCGCCGGGAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGCGAGCAATGTAAGgt                                                                           >  1:179741/1‑140 (MQ=255)                                                              tGAAAGCGCCGGGAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGCGAGCAATGTAAGgtg                                                                          <  2:126288/140‑1 (MQ=255)                                                                         ggAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGCGAGCAATGTAAGGTGTATCGGGGAAt                                                               <  2:167429/140‑1 (MQ=255)                                                                                cACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGCGAGCAATGTAAGGTGTATCGGGGAATTGTGCtt                                                        >  1:198291/1‑140 (MQ=255)                                                                                     aaaCTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGCGAGCAATGTAAGGTGTATCGGGGAATTGTGCTTTCAGt                                                   >  1:532502/1‑140 (MQ=255)                                                                                      aaCTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGCGAGCAATGTAAGGTGTATCGGGGAATTGTGCTTTCAGtt                                                  <  2:38331/140‑1 (MQ=255)                                                                                       aCTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGCGAGCAATGTAAGGTGTATCGGGGAATTGTGCTTTCAGTTc                                                 <  1:694550/140‑1 (MQ=255)                                                                                             gTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGCGAGCAATGTAAGGTGTATCGGGGAATTGTGCTTTCAGTTCTGGAAc                                           >  1:227879/1‑140 (MQ=255)                                                                                                        ggCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGCGAGCAATGTAAGGTGTATCGGGGAATTGTGCTTTCAGTTCTGGAACTAATgg                                     >  2:318747/1‑135 (MQ=255)                                                                                                        ggCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGCGAGCAATGTAAGGTGTATCGGGGAATTGTGCTTTCAGTTCTGGAACTAATgg                                     <  1:631040/135‑1 (MQ=255)                                                                                                        ggCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGCGAGCAATGTAAGGTGTATCGGGGAATTGTGCTTTCAGTTCTGGAACTAATgg                                     >  2:631040/1‑135 (MQ=255)                                                                                                        ggCAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGCGAGCAATGTAAGGTGTATCGGGGAATTGTGCTTTCAGTTCTGGAACTAATgg                                     <  1:318747/135‑1 (MQ=255)                                                                                                          cAATAATTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGCGAGCAATGTAAGGTGTATCGGGGAATTGTGCTTTCAGTTCAAGAACTAATGGGCCGTTa                              >  2:160481/1‑140 (MQ=255)                                                                                                               atTAACTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGCGAGCAATGTAAGGTGTATCGGGGAATTGTGCTTTCAGTTCTGGAACTAATGGGCCGTTAGGAcc                         >  1:795368/1‑140 (MQ=255)                                                                                                                   aCTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGCGAGCAATGTAAGGTGTATCGGGGAATTGTGCTTTCAGTTCTGGAACTAATGGGCCGTTAGGACCGGtt                     >  2:772105/1‑140 (MQ=255)                                                                                                                   aCTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGCGAGCAATGTAAGGTGTATCGGGGAATTGTGCTTTCAGTTCTGGAACTAATGGGCCGTTAGGACCGGtt                     >  2:663008/1‑140 (MQ=255)                                                                                                                   aCTGTTTTTTACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAACATTTCCCGGGCGAGCAATGTAAGGTGTATCGGGGAATTGTGCTTTCAGTTCTGGAACTAATGGGCCGTTAGGACCGGtt                     >  2:894278/1‑140 (MQ=255)                                                                                                                       ttttttACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGCGAGCAATGTAAGGTGTATCGGGGAATTGTGCTTTCAGTTCTGGAACTAATGGGCCGTTAGGACCGGTTTCaa                 >  1:678075/1‑140 (MQ=255)                                                                                                                         ttttACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGCGAGCAATGTAAGGTGTATCGGGGAATTGTGCTTTCAGTTCTGGAACTAATGGGCCGTTAGGACCGGTTTCaaaa               <  1:163780/140‑1 (MQ=255)                                                                                                                          tttACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGCGAGCAATGTAAGGTGTATCGGGGAATTGTGCTTTCAGTTCTGGAACTAATGGGCCGTTAGGACCGGTTTCAAAAc              >  1:53527/1‑140 (MQ=255)                                                                                                                          tttACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGCGAGCAATGTAAGGTGTATCGGGGAATTGTGCTTTCAGTTCTGGAACTAATGGGCCGTTAGGACCGGTTTCAAAAc              <  2:532502/140‑1 (MQ=255)                                                                                                                            tACCTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGCGAGCAATGTAAGGTGTATCGGGGAATTGTGCTTTCAGTTCTGGAACTAATGGGCCGTTAGGACCGGTTTCAAAACtg            <  1:663008/140‑1 (MQ=255)                                                                                                                              ccTGTCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGCGAGCAATGTAAGGTGTATCGGGGAATTGTGCTTTCAGTTCTGGAACTAATGGGCCGTTAGGACCGGTTTCAAAACtggt          <  1:1093480/140‑1 (MQ=255)                                                                                                                                  tCGCTTTGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGCGAGCAATGTAAGGTGTATCGGGGAATTGTGCTTTCAGTTCTGGAACTAATGGGCCGTTAGGACCGGTTTCAAAACTGGTGGTa      <  1:984826/140‑1 (MQ=255)                                                                                                                                      tttGGCAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGCGAGCAATGTAAGGTGTATCGGGGAATTGTGCTTTCAGTTCTGGAACTAATGGGCCGTTAGGACCGGTTTCAAAACTGGTGGTAAGaa  <  1:772105/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        TTTCATTAAAGGTCCCTGACGCGTCAGTCACGACAAAGACGTCAAACCCTTCTTCAATGGCTGAAAGCGCCGGGAATGCCACGCAAACTTCGGTTACCACACCGGCAATAATTAACTGTTTTTTACCTGTCGCTTTGACAGCTTTTACAAAATCTTCGTTATCCCAGGCGTTAATATTTCCCGGGCGAGCAATGTAAGGTGTATCGGGGAATTGTGCTTTCAGTTCTGGAACTAATGGGCCGTTAGGACCGGTTTCAAAACTGGTGGTAAGAA  >  NC_000913/945122‑945394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑