Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,100,097	C→G	A153G (GCC→GGC)	ycdY →	chaperone protein YcdY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,100,097	0	C	G	100.0%	244.1 / NA	68	A153G (GCC→GGC)	ycdY	chaperone protein YcdY
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (36/32);  total (36/32)

ATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATA  >  NC_000913/1099967‑1100228                                                                                                                                   |                                                                                                                                    aTCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAAc                                                                                                                            <  1:1033434/140‑1 (MQ=255) aTCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAAc                                                                                                                            <  2:1032259/140‑1 (MQ=255)   cGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCa                                                                                                                          >  1:558786/1‑140 (MQ=255)           tgctgcTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCAt                                                                                                                                 >  1:745000/1‑125 (MQ=255)           tgctgcTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCAt                                                                                                                                 <  2:745000/125‑1 (MQ=255)                ctCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGgcg                                                                                                             <  1:1094748/140‑1 (MQ=255)                ctCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGgcg                                                                                                             >  2:738288/1‑140 (MQ=255)                 tCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAAc                                                                                                                            <  1:801162/124‑1 (MQ=255)                 tCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAAc                                                                                                                            >  2:801162/1‑124 (MQ=255)                 tCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAAc                                                                                                                            <  1:481798/124‑1 (MQ=255)                 tCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAAc                                                                                                                            >  2:481798/1‑124 (MQ=255)                         tCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGc                                                                                                    <  1:218200/140‑1 (MQ=255)                             ggctggAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCg                                                                                                <  1:122034/140‑1 (MQ=255)                                 ggAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTaa                                                                                            >  2:12513/1‑140 (MQ=255)                                     gATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCcg                                                                                        <  1:508981/140‑1 (MQ=255)                                        cAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGa                                                                                     >  1:918207/1‑140 (MQ=255)                                             aaCGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCa                                                                                >  1:1000102/1‑140 (MQ=255)                                              aCGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCAt                                                                               <  2:189404/140‑1 (MQ=255)                                              aCGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCAt                                                                               >  1:189404/1‑140 (MQ=255)                                               cGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCAtt                                                                              >  2:492350/1‑140 (MQ=255)                                                  aaGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAAccc                                                                                         >  1:912521/1‑126 (MQ=255)                                                  aaGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAAccc                                                                                         <  2:912521/126‑1 (MQ=255)                                                  aaGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGt                                                                           >  2:17068/1‑140 (MQ=255)                                                  aaGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGt                                                                           >  2:526054/1‑140 (MQ=255)                                                  aaGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGt                                                                           >  1:1023564/1‑140 (MQ=255)                                                  aaGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGt                                                                           <  1:1048142/140‑1 (MQ=255)                                                   aGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTg                                                                          <  1:12513/140‑1 (MQ=255)                                                   aGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTg                                                                          <  2:918207/140‑1 (MQ=255)                                                   aGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTg                                                                          <  2:1023564/140‑1 (MQ=255)                                                     aTGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAGGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCcgc                                                                                       <  2:1057748/125‑1 (MQ=255)                                                     aTGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCcgc                                                                                       >  1:1057748/1‑125 (MQ=255)                                                     aTGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGAt                                                                                    <  1:466329/128‑1 (MQ=255)                                                     aTGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGAt                                                                                    >  2:466329/1‑128 (MQ=255)                                                             gAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGa                                                                <  1:345898/140‑1 (MQ=255)                                                              aaGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGAc                                                               <  2:558786/140‑1 (MQ=255)                                                               aGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACg                                                              >  2:399586/1‑140 (MQ=255)                                                               aGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACg                                                              >  2:621032/1‑140 (MQ=255)                                                                gCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGGGGGCCATGTAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACg                                                              >  2:408926/1‑139 (MQ=255)                                                                gCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGGGGGCCATGTAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACg                                                              <  1:408926/139‑1 (MQ=255)                                                                             tGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAt                                                >  2:160/1‑140 (MQ=255)                                                                                 cAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTg                                            <  1:621032/140‑1 (MQ=255)                                                                                 cAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTg                                            <  1:399586/140‑1 (MQ=255)                                                                                    tGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGGAGAAGATTCTgaag                                         >  2:1083357/1‑140 (MQ=255)                                                                                    tGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTgaag                                         >  1:917380/1‑140 (MQ=255)                                                                                       gTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAAt                                      <  1:738288/140‑1 (MQ=255)                                                                                         aTCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATaa                                    >  2:703459/1‑140 (MQ=255)                                                                                         aTCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATaa                                    >  1:937380/1‑140 (MQ=255)                                                                                           cTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAgt                                  <  2:628755/140‑1 (MQ=255)                                                                                              ttACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAgt                                  >  2:311149/1‑137 (MQ=255)                                                                                              ttACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAgt                                  <  1:311149/137‑1 (MQ=255)                                                                                                aCCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGAt                             >  2:39477/1‑140 (MQ=255)                                                                                                aCCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGAt                             >  1:887153/1‑140 (MQ=255)                                                                                                  ccTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAAt                                      <  2:749662/129‑1 (MQ=255)                                                                                                  ccTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAAt                                      >  1:749662/1‑129 (MQ=255)                                                                                                  ccTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCt                           >  2:966264/1‑140 (MQ=255)                                                                                                     ggtgtggtgCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACg                        <  2:917380/140‑1 (MQ=255)                                                                                                     ggtgtggtgCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACg                        <  1:639435/140‑1 (MQ=255)                                                                                                       tgtggtgCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTc                      >  1:192049/1‑140 (MQ=255)                                                                                                        gtggtgCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCa                     <  1:39477/140‑1 (MQ=255)                                                                                                        gtggtgCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCa                     >  1:700150/1‑140 (MQ=255)                                                                                                         tggtgCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCAc                    >  2:25997/1‑140 (MQ=255)                                                                                                          ggtgCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACt                   <  1:526054/140‑1 (MQ=255)                                                                                                            tgCGTTCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCa                 >  2:61373/1‑140 (MQ=255)                                                                                                                ttCCTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAAc             >  1:981273/1‑140 (MQ=255)                                                                                                                   cTTGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGc          >  1:578782/1‑140 (MQ=255)                                                                                                                     tGGCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCaa        <  1:703459/140‑1 (MQ=255)                                                                                                                      ggCAAAGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAAc       <  1:61373/140‑1 (MQ=255)                                                                                                                           aGTGGAGGGCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATa  >  2:101966/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                    ATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATA  >  NC_000913/1099967‑1100228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑