Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,106,134	A→G	L105L (CTA→CTG)	ymdB →	2'‑O‑acetyl‑ADP‑ribose deacetylase, regulator of RNase III activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,106,134	0	A	G	100.0%	134.9 / NA	38	L105L (CTA→CTG)	ymdB	2'‑O‑acetyl‑ADP‑ribose deacetylase, regulator of RNase III activity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/17);  total (21/17)

ACGGGGCATGCCGTTATTACGCTTGCAGGCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAGGATGCCTATCTCAATAGCCTACGACTGGTGGCGGCAAACAGCTATACGTCAGTGGCTTTTCCTGCAATCAGTACTGGGGTTTATGGTTACCCTCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTAC  >  NC_000913/1106003‑1106270                                                                                                                                    |                                                                                                                                         aCGGGGCATGCCGTTATTACGCTTGCAGGCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAGGATGCCTATCTCAATAGCCTGCGACtggt                                                                                                                                  >  2:158528/1‑140 (MQ=255)  cGGGGCATGCCGTTATTACGCTTGCAGGCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAGGATGCCTATCTCAATAGCCTGCGACtggtg                                                                                                                                 >  2:266039/1‑140 (MQ=255)       cATGCCGTTATTACGCTTGCAGGCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAGGATGCCTATCTCAATAGCCTGCGACTGGTggcggc                                                                                                                            >  2:530286/1‑140 (MQ=255)       cATGCCGTTATTACGCTTGCAGGCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAGGATGCCTATCTCAATAGCCTGCGACTGGTggcggc                                                                                                                            >  2:530682/1‑140 (MQ=255)        aTGCCGTTATTACGCTTGCAGGCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAGGATGCCTATCTCAATAGCCTGCGACTGGTGGCGGCa                                                                                                                           <  2:888925/140‑1 (MQ=255)        aTGCCGTTATTACGCTTGCAGGCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAGGATGCCTATCTCAATAGCCTGCGACTGGTGGCGGCa                                                                                                                           <  1:266039/140‑1 (MQ=255)         tGCCGTTATTACGCTTGCAGGCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAGGATGCCTATCTCAATAGCCTGCGACTGGTGGCGGCaa                                                                                                                          <  2:588324/140‑1 (MQ=255)          gCCGTTATTACGCTTGCAGGCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAGGATGCCTATCTCAATAGCCTGCGACTGGTGGCGGCaaa                                                                                                                         <  2:175224/140‑1 (MQ=255)           ccGTTATTACGCTTGCAGGCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAGGATGCCTATCTCAATAGCCTGCGACTGGTGGCGGCAAAc                                                                                                                        <  2:563168/140‑1 (MQ=255)                     gCTTGCAGGCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAGGATGCCTATCTCAATAGCCTGCGACTGGTGGCGGCAAACAGCTATACGt                                                                                                              <  2:658077/140‑1 (MQ=255)                         gCAGGCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAGGATGCCTATCTCAATAGCCTGCGACTGGTGGCGGCAAACAGCTATACGTCAGt                                                                                                          >  1:298589/1‑140 (MQ=255)                         gCAGGCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAGGATGCCTATCTCAATAGCCTGCGACTGGTGGCGGCAAACAGCTATACGTCAGt                                                                                                          >  1:44871/1‑140 (MQ=255)                           aGGCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAGGATGCCTATCTCAATAGCCTGCGACTGGTGGCGGCaaa                                                                                                                         <  2:755124/123‑1 (MQ=255)                           aGGCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAGGATGCCTATCTCAATAGCCTGCGACTGGTGGCGGCaaa                                                                                                                         >  1:755124/1‑123 (MQ=255)                            ggCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAGGATGCCTATCTCAATAGCCTGCGACTGGTGGCGGCAAACAGCTATACGTCAGTGGc                                                                                                       >  1:574676/1‑140 (MQ=255)                                   ttCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAGGATGCCTATCTCAATAGCCTGCGACTGGTGGCGGCAAACAGCTATACGTCAGTGGCTTTTCCt                                                                                                <  2:44871/140‑1 (MQ=255)                                   ttCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAGGATGCCTATCTCAATAGCCTGCGACTGGTGGCGGCAAACAGCTATACGTCAGTGGCTTTTCCt                                                                                                <  1:158528/140‑1 (MQ=255)                                   ttCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAGGATGCCTATCTCAATAGCCTGCGACTGGTGGCGGCAAACAGCTATACGTCAGTGGCTTTTCCt                                                                                                <  1:185581/140‑1 (MQ=255)                                       cGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAGGATGCCTATCTCAATAGCCTGCGACTGGTGGCGGCAAACAGCTATACGTCAGTGGCTTTTCCTGCaa                                                                                            >  2:339895/1‑140 (MQ=255)                                        gCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAGGATGCCTATCTCAATAGCCTGCGACTGGTGGCGGCAAACAGCTATACGTCAGTGGCTTTTCCTGCAAt                                                                                           <  1:367623/140‑1 (MQ=255)                                        gCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAGGATGCCTATCTCAATAGCCTGCGACTGGTGGCGGCAAACAGCTATACGTCAGTGGCTTTTCCTGCAAt                                                                                           <  2:298589/140‑1 (MQ=255)                                               ccGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAGGATGCCTATCTCAATAGCCTGCGACTGGTGGCGGCAAACAGCTATACGTCAGTGGCTTTTCCTGCAATCAGTACt                                                                                    <  1:502576/140‑1 (MQ=255)                                                cGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAGGATGCCTATCTCAATAGCCTGCGACTGGTGGCGGCAAACAGCTATACGTCAGTGGCTTTTCCTGCAATCAGTACTg                                                                                   <  2:106849/140‑1 (MQ=255)                                                   aGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAGGATGCCTATCTCAATAGCCTGCGACTGGTGGCGGCAAACAGCTATACGTCAGTGGCTTTTCCTGCAATCAGTACt                                                                                    <  2:612229/136‑1 (MQ=255)                                                   aGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAGGATGCCTATCTCAATAGCCTGCGACTGGTGGCGGCAAACAGCTATACGTCAGTGGCTTTTCCTGCAATCAGTACt                                                                                    >  1:612229/1‑136 (MQ=255)                                                      gCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAGGATGCCTATCTCAATAGCCTGCGACTGGTGGCGGCAAACAGCTATACGTCAGTGGCTTTTCCTGCAATCAGTACTGGGGttt                                                                             <  1:530286/140‑1 (MQ=255)                                                               cGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAGGATGCCTATCTCAATAGCCTGCGACTGGTGGCGGCAAACAGCTATACGTCAGTGGCTTTTCCTGCAATCAGTACTGGGGTTTATGGTTAcc                                                                    >  1:844051/1‑140 (MQ=255)                                                                                gtggtgAACAAAACGAAGACCAGCTTTTGCAGGATGCCTATCTCAATAGCCTGCGACTGGTGGCGGCAAACAGCTATACGTCAGTGGCTTTTCCTGCAATCAGTACTGGGGTTTATGGTTACCCTCGTGCGGCAGCGGCt                                                   >  2:1023695/1‑140 (MQ=255)                                                                                 tggtgAACAAAACGAAGACCAGCTTTTGCAGGATGCC‑‑TCTCAATAGCCTGCGACTGGTGGCGGCAAACAGCTATACGTCAGTGGCTTTTCCTGCAATCAGTACTGGGGTTTATGGTTACCCTCGTGCGGCAGCGGCTGaa                                                >  1:446040/1‑140 (MQ=255)                                                                                           aaCGAAGACCAGCTTTTGCAGGATGCCTATCTCAATAGCCTGCGACTGGTGGCGGCAAACAGCTATACGTCAGTGGCTTTTCCTGCAATCAGTACTGGGGTTTATGGTTACCCTCGTGCGGCAGCGGCTGAAATCGCAGt                                        >  2:642707/1‑140 (MQ=255)                                                                                           aaCGAAGACCAGCTTTTGCAGGATGCCTATCTCAATAGCCTGCGACTGGTGGCGGCAAACAGCTATACGTCAGTGGCTTTTCCTGCAATCAGTACTGGGGTTTATGGTTACCCTCGTGCGGCAGCGGCTGAAATCGCAGt                                        >  1:311860/1‑140 (MQ=255)                                                                                           aaCGAAGACCAGCTTTTGCAGGATGCCTATCTCAATAGCCTGCGACTGGTGGCGGCAAACAGCTATACGTCAGTGGCTTTTACTGCAATCAGTACTGGGGTTTATGGTTACCCTCGTGCGGCAGCGGCTGAAATCGCAGt                                        >  1:541719/1‑140 (MQ=255)                                                                                                            gCAGGATGCCTATCTCAATAGCCTGCGACTGGTGGCGGCAAACAGCTATACGTCAGTGGCTTTTCCTGCAATCAGTACTGGGGTTTATGGTTACCCTCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAAt                       >  1:997169/1‑140 (MQ=255)                                                                                                                 ggatgcctctcAATAGCCTGCGACTGGTGGCGGCAAACAGCTATACGTCAGTGGCTTTTCCTGCAATCAGTACTGGGGTTTATGGTTACCCTCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATttatt                  <  2:446040/133‑1 (MQ=255)                                                                                                                    ccTATCTCAATAGCCTGCGACTGGTGGCGGCAAACAGCTATACGTCAGTGGCTTTTCCTGCAATCAGTACTGGGGTTTATGGTTACCCTCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTAcc               >  1:1007458/1‑140 (MQ=255)                                                                                                                       aTCTCAATAGCCTGCGACTGGTGGCGGCAAACAGCTATACGTCAGTGGCTTTTCCTGCAATCAGTACTGGGGTTTATGGTTACCCTCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGt            >  1:549807/1‑140 (MQ=255)                                                                                                                              tAGCCTGCGACTGGTGGCGGCAAACAGCTATACGTCAGTGGCTTTTCCTGCAATCAGTACTGGGGTTTATGGTTACCCTCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCtt     >  1:799505/1‑140 (MQ=255)                                                                                                                                 ccTGCGACTGGTGGCGGCAAACAGCTATACGTCAGTGGCTTTTCCTGCAATCAGTACTGGGGTTTATGGTTACCCTCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTAc  >  1:196592/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                         ACGGGGCATGCCGTTATTACGCTTGCAGGCGATCTTCCCGCTAAAGCCGTAGTGCACACCGTCGGGCCAGTCTGGCGTGGTGGTGAACAAAACGAAGACCAGCTTTTGCAGGATGCCTATCTCAATAGCCTACGACTGGTGGCGGCAAACAGCTATACGTCAGTGGCTTTTCCTGCAATCAGTACTGGGGTTTATGGTTACCCTCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTAC  >  NC_000913/1106003‑1106270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑