Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,291,035	T→C	V265A (GTT→GCT)	rssB →	regulator of RpoS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,291,035	0	T	C	100.0%	207.9 / NA	58	V265A (GTT→GCT)	rssB	regulator of RpoS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/30);  total (28/30)

ACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGG  >  NC_000913/1290898‑1291155                                                                                                                                          |                                                                                                                         aCGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTg                                                                                                                        >  1:536828/1‑140 (MQ=255)    cgcATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCt                                                                                                                     >  2:367379/1‑140 (MQ=255)     gcATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCta                                                                                                                    <  2:536828/140‑1 (MQ=255)     gcATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGACAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCta                                                                                                                    >  1:385525/1‑140 (MQ=255)      cATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCtat                                                                                                                   >  2:560220/1‑140 (MQ=255)      cATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCtat                                                                                                                   <  2:866608/140‑1 (MQ=255)       aTTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCtatt                                                                                                                  >  1:242100/1‑140 (MQ=255)                 ggATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGa                                                                                                        >  1:699353/1‑140 (MQ=255)                   attattACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAAc                                                                                                      <  1:215746/140‑1 (MQ=255)                     tattaCAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATcgcg                                                                                                         <  2:46685/135‑1 (MQ=255)                     tattaCAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATcgcg                                                                                                         >  1:46685/1‑135 (MQ=255)                     tattaCAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTg                                                                                                    >  2:1063975/1‑140 (MQ=255)                       ttaCAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGaa                                                                                                  <  2:1025228/140‑1 (MQ=255)                       ttaCAGGAACAGCTCGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGaa                                                                                                  <  2:905863/140‑1 (MQ=255)                             gAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAAtct                                                                                            >  1:800783/1‑140 (MQ=255)                                 aGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCAtt                                                                                        >  1:931609/1‑140 (MQ=255)                                  gCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTc                                                                                       >  1:598450/1‑140 (MQ=255)                                   cTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCt                                                                                      <  1:864933/140‑1 (MQ=255)                                   cTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCt                                                                                      >  2:744349/1‑140 (MQ=255)                                     tGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTgg                                                                                    >  1:969794/1‑140 (MQ=255)                                     tGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTgg                                                                                    >  1:309690/1‑140 (MQ=255)                                           ccAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTg                                                                              <  2:242100/140‑1 (MQ=255)                                                aTCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGt                                                                         <  1:556590/140‑1 (MQ=255)                                                aTCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGt                                                                         <  1:560220/140‑1 (MQ=255)                                                    aCGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGa                                                                     >  2:397791/1‑140 (MQ=255)                                                        ttGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTAGCATCGCAAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGc                                                                 <  2:385525/140‑1 (MQ=255)                                                         tGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCg                                                                >  2:932983/1‑140 (MQ=255)                                                         tGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCg                                                                <  1:367379/140‑1 (MQ=255)                                                          gCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGa                                                               <  1:1014371/140‑1 (MQ=255)                                                           ccAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTg                                                                      <  2:213181/132‑1 (MQ=255)                                                           ccAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTg                                                                      >  1:213181/1‑132 (MQ=255)                                                               agTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTa                                                          >  1:1059553/1‑140 (MQ=255)                                                                   gggCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATa                                                      <  2:598450/140‑1 (MQ=255)                                                                    ggCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCACGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATAc                                                     >  1:187902/1‑140 (MQ=255)                                                                    ggCGCGTTATTGAAGCAGGTAAACCATTTACTTCATCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCCGCGGGTCTGAATGCGACGTTAAATAc                                                     >  2:969831/1‑140 (MQ=255)                                                                         gTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGTGGGTCTGAATGCGACGTTAAATACCGGCg                                                >  1:954443/1‑140 (MQ=255)                                                                         gTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCg                                                >  1:576722/1‑140 (MQ=255)                                                                          ttATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGTGGGTCTGAATGCGACGTTAAATACCGGCGa                                               <  2:954443/140‑1 (MQ=255)                                                                                 aGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGCTCTGAATGCGACGTTAAATACCGGCGAACACCAg                                        <  2:969794/140‑1 (MQ=255)                                                                                    aGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTg                                     <  2:931609/140‑1 (MQ=255)                                                                                     ggTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGc                                    >  1:610729/1‑140 (MQ=255)                                                                                       tAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCaa                                  <  1:1063975/140‑1 (MQ=255)                                                                                       tAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCaa                                  <  2:1059553/140‑1 (MQ=255)                                                                                       tAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCaa                                  <  1:969831/140‑1 (MQ=255)                                                                                               ttACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTaa                          <  2:576722/140‑1 (MQ=255)                                                                                                tACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCaa                                  <  2:486432/131‑1 (MQ=255)                                                                                                tACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCaa                                  >  1:486432/1‑131 (MQ=255)                                                                                                tACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAAt                         >  2:1018105/1‑140 (MQ=255)                                                                                                 aCTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGATAAATACCGGCGAACACCAGGTGCAAATCAGTaa                          <  2:472970/138‑1 (MQ=255)                                                                                                 aCTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGATAAATACCGGCGAACACCAGGTGCAAATCAGTaa                          >  1:472970/1‑138 (MQ=255)                                                                                                     cGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGgtgt                    >  1:973295/1‑140 (MQ=255)                                                                                                           gCCAATCTGCCGGGTCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGtt              <  2:432338/140‑1 (MQ=255)                                                                                                              aaTCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAgg           <  1:744349/140‑1 (MQ=255)                                                                                                              aaTCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAgg           <  2:800783/140‑1 (MQ=255)                                                                                                              aaTCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAgg           <  1:397791/140‑1 (MQ=255)                                                                                                              aaTCTGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAgg           <  2:309690/140‑1 (MQ=255)                                                                                                                  tGCCGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCACGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACt       <  2:187902/140‑1 (MQ=255)                                                                                                                     cGGGGCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTa    >  2:319423/1‑140 (MQ=255)                                                                                                                       gggCAGTTTCCGCTATTAGCTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAgg  <  2:803100/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                         ACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGG  >  NC_000913/1290898‑1291155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑