Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,323,030	T→C	intergenic (‑84/+8)	trpE ← / ← trpL	anthranilate synthase subunit TrpE/trp operon leader peptide

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,323,030	0	T	C	100.0%	112.8 / NA	32	intergenic (‑84/+8)	trpE/trpL	anthranilate synthase subunit TrpE/trp operon leader peptide
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/15);  total (17/15)

GATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATTGTTATTCTCTAATTTTGTTCAAAAAAAAGCCCGCTCATTAGGCGGGCTGGGTATCTGATTGCTTTACGCATGGTGAATACACTGCCCGTTTCAGGAAGTGCGCCACCAACCTTTCAGTACGAAAATTGCTTTCATTGTCGATACCCTTTTTACGTGAACTTGCGTACTAGTTAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATAT  >  NC_000913/1322895‑1323162                                                                                                                                        |                                                                                                                                     gATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATTGTTACTCTCTAATTTTGTTCAAAAAAAAGCCCGCTCATTAGGCGGGCTGGGTATCTGATTGCTTTACGCATGGTGAATACACCGccc                                                                                                                                  <  2:895089/140‑1 (MQ=255)  aTAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATTGTTATTCTCTAATTTTGTTCAAAAAAAAGCCCGCTCATTAGGCGGGCTGGGTATCTGATTGCTTTACGCATGGTGAATACACCGCCCg                                                                                                                                 >  2:915828/1‑140 (MQ=255)           ttCGCAGGTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATTGTTATTCTCTAATTTTGTTCAAAAAAAAGCCCGCTCATTAGGCGGGCTGGGTATCTGATTGCTTTACGCATGGTGAATACACCGCCCGTTTCAGGaa                                                                                                                        >  1:1046654/1‑140 (MQ=255)             cGCAGGTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATTGTTATTCTCTAATTTTGTTCAAAAAAAAGCCCGCTCATTAGGCGGGCTGGGTATCTGATTGCTTTACGCATGGTGAATACACCGCCCGTTTCAGGAAGt                                                                                                                      >  2:899891/1‑140 (MQ=255)                aGGTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATTGTTATTCTCTAATTTTGTTCAAAAAAAAGCCCGCTCATTAGGCGGGCTGGGTATCTGATTGCTTTACGCATGGTGAATACACCGCCCGTTTCAGGAAGTgcg                                                                                                                   <  1:1093447/140‑1 (MQ=255)                  gTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATTGTTATTCTCTAATTTTGTTCAAAAAAAAGCCCGCTCATTAGGCGGGCTGGGTATCTGATTGCTTTACGCATGGTGAATACACCGCCCGTTTCAGGAAGTGCGcc                                                                                                                 <  1:923031/140‑1 (MQ=255)                               gagTCGGTTTTTGTGTTTGCATTGTTATTCTCTAATTTTGTTCAAAAAAAAGCCCGCTCATTAGGCGGGCTGGGTATCTGATTGCTTTACGCATGGTGAATACACCGCCCGTTTCAGGAAGTGCGccacc                                                                                                              >  2:1008227/1‑130 (MQ=255)                               gagTCGGTTTTTGTGTTTGCATTGTTATTCTCTAATTTTGTTCAAAAAAAAGCCCGCTCATTAGGCGGGCTGGGTATCTGATTGCTTTACGCATGGTGAATACACCGCCCGTTTCAGGAAGTGCGccacc                                                                                                              <  1:1008227/130‑1 (MQ=255)                                    ggTTTTTGTGTTTGCATTGTTATTCTCTAATTTTGTTCAAAAAAAAGCCCGCTCATTAGGCGGGCTGGGTATCTGATTGCTTTACGCATGGTGAATACACCGCCCGTTTCAGGAAGTGCGCCACCAACCTTTCAGTACGa                                                                                               >  2:900942/1‑140 (MQ=255)                                    ggTTTTTGTGTTTGCATTGTTATTCTCTAATTTTGTTCAAAAAAAAGCCCGCTCATTAGGCGGGCTGGGTATCTGATTGCTTTACGCATGGTGAATACACCGCCCGTTTCAGGAAGTGCGCCACCAACCTTTCAGTACGa                                                                                               >  2:11248/1‑140 (MQ=255)                                                tGCATTGTTATTCTCTAATTTTGTTCAAAAAAAAGCCCGCTCATTAGGCGGGCTGGGTATCTGATTGCTTTACGCATGGTGAATACACCGCCCGTTTCAGGAAGTGCGCCACCAACCTTTCAGTACGAAAATTGCTTTCa                                                                                   <  1:900942/140‑1 (MQ=255)                                                    ttGTTATTCTCTAATTTTGTTCAAAAAAAAGCCCGCTCATTAGGCGGGCTGGGTATCTGATTGCTTTACGCATGGTGAATACACCGCCCGTTTCAGGAAGTGCGCCACCAACCTTTCAGTACGAAAATTGCTTTCATTGt                                                                               <  2:1046654/140‑1 (MQ=255)                                                      gTTATTCTCTAATTTTGTTCAAAAAAAAGCCCGCTCATTAGGCGGGCTGGGTATCTGATTGCTTTACGCATGGTGAATACACCGCCCGTTTCAGGAAGTGCGCCACCAACCTTTCAGTACGAAAATTGCTTTCATTGTCg                                                                             <  1:11248/140‑1 (MQ=255)                                                               tAATTTTGTTCAAAAAAAAGCCCGCTCATTAGGCGGGCTGGGTATCTGATTGCTTTACGCATGGTGAATACACCGCCCGTTTCAGGAAGTGCGCCACCAACCTTTCAGTACGAAAATTGCTTTCATTGTCGATACCCttt                                                                    >  1:436629/1‑140 (MQ=255)                                                               tAATTTTGTTCAAAAAAAAGCCCGCTCATTAGGCGGGCTGGGTATCTGATTGCTTTACGCATGGTGAATACACCGCCCGTTTCAGGAAGTGCGCCACCAACCTTTCAGTACGAAAATTGCTTTCATTGTCGATACCCttt                                                                    >  1:712230/1‑140 (MQ=255)                                                                 aTTTTGTTCAAAAAAAAGCCCGCTCATTAGGCGGGCTGGGTATCTGATTGCTTTACGCATGGTGAATACACCGCCCGTTTCAGGAAGTGCGCCACCAACCTTTCAGTACGAAAATTGCTTTCATTGTCGATACCCttttt                                                                  >  1:555552/1‑140 (MQ=255)                                                                 aTTTTGTTCAAAAAAAAGCCCGCTCATTAGGCGGGCTGGGTATCTGATTGCTTTACGCATGGTGAATACACCGCCCGTTTCAGGAAGTGCGCCACCAACCTTTCAGTACGAAAATTGCTTTCATTGTCGATACCCttttt                                                                  >  2:597099/1‑140 (MQ=255)                                                                 aTTTTGTTCAAAAAAAAGCCCGCTCATTAGGCGGGCTGGGTATCTGATTGCTTTACGCATGGTGAATACACCGCCCGTTTCAGGAAGTGCGCCACCAACCTTTCAGTACGAAAATTGCTTTCATTGTCGATACCCttttt                                                                  >  2:739794/1‑140 (MQ=255)                                                                       ttCAAAAAAAAGCCCGCTCATTAGGCGGGCTGGGTATCTGATTGCTTTACGCATGGTGAATACACCGCCCGTTTCAGGAAGTGCGCCACCAACCTTTCAGTACGAAAATTGCTTTCATTGTCGATACCCTTTTTACGTGa                                                            >  1:58576/1‑140 (MQ=255)                                                                       ttCAAAAAAAAGCCCGCTCATTAGGCGGGCTGGGTATCTGATTGCTTTACGCATGGTGAATACACCGCCCGTTTCAGGAAGTGCGCCACCAACCTTTCAGTACGAAAATTGCTTTCATTGTCGATACCCTTTTTACGTGa                                                            >  2:721099/1‑140 (MQ=255)                                                                               aaaGCCCGCTCATTAGGCGGGCTGGGTATCTGATTGCTTTACGCATGGTGAATACACCGCCCGTTTCAGGAAGTGCGCCACCAACCTTTCAGTACGAAAATTGCTTTCATTGTCGATACCCTTTTTACGTGAACTTGCGt                                                    <  1:899891/140‑1 (MQ=255)                                                                                    ccGCTCATTAGGCGGGCTGGGTATCTGATTGCTTTACGCATGGTGAATACACCGCCCGTTTCAGGAAGTGCGCCACCAACCTTTCAGTACGAAAATTGCTTTCATTGTCGATACCCTTTTTACGTGAACTTGCGTACTAg                                               <  2:436629/140‑1 (MQ=255)                                                                                                  ggCTGGGTATCTGATTGCTTTACGCATGGTGAATACACCGCCCGTTTCAGGAAGTGCGCCACCAACCTTTCAGTACGAAAATTGCTTTCATTGTCGATACCCTTTTTACGTGAACTTGCGTACTAGTTAACTAGTTCgat                                 >  1:538192/1‑140 (MQ=255)                                                                                                  ggCTGGGTATCTGATTGCTTTACGCATGGTGAATACACCGCCCGTTTCAGGAAGTGCGCCACCAACCTTTCAGTACGAAAATTGCTTTCATTGTCGATACCCTTTTTACGTGAACTTGCGTACTAGTTAACTAGTTCgat                                 >  1:202949/1‑140 (MQ=255)                                                                                                     tGGGTATCTGATTGCTTTACGCATGGTGAATACACCGCCCGTTTCAGGAAGTGCGCCACCAACCTTTCAGTACGAAAATTGCTTTCATTGTCGATACCCTTTTTACGTGAACTTGCGTACTAGTTAACTAGTTCgatgat                              <  2:202949/140‑1 (MQ=255)                                                                                                     tGGGTATCTGATTGCTTTACGCATGGTGAATACACCGCCCGTTTCAGGAAGTGCGCCACCAACCTTTCAGTACGAAAATTGCTTTCATTGTCGATACCCTTTTTACGTGAACTTGCGTACTAGTTAACTAGTTCgatgat                              <  2:538192/140‑1 (MQ=255)                                                                                                            cTGATTGCTTTACGCATGGTGAATACACCGCCCGTTTCAGGAAGTGCGCCACCAACCTTTCAGTACGAAAATTGCTTTCATTGTCGATACCCTTTTTACGTGAACTTGCGTACTAGTTAACTAGTTCGATGATTAATTGt                       <  1:943025/140‑1 (MQ=255)                                                                                                               aTTGCTTTACGCATGGTGAATACACCGCCCGTTTCAGGAAGTGCGCCACCAACCTTTCAGTACGAAAATTGCTTTCATTGTCGATACCCTTTTTACGTGAACTTGCGTACTAGTTAACTAGTTCGATGATTAATTGTCaa                    <  1:688098/140‑1 (MQ=255)                                                                                                                 tGCTTTACGCATGGTGAATACACCGCCCGTTTCAGGAAGTGCGCCACCAACCTTTCAGTACGAAAATTGCTTTTATTGTCGATACCCTTTTTACGTTAACTTGCGTACTAGTTAACTAGTTCGATGATTAATTGTCAACa                  >  2:954663/1‑140 (MQ=255)                                                                                                                       aCGCATGGTGAATACACCGCCCGTTTCAGGAAGTGCGCCACCAACCTTTCAGTACGAAAATTGCTTTCATTGTCGATACCCTTTTTACGTGAACTTGCGTACTAGTTAACTAGTTCGATGATTAATTGTCAACAGCTCAt            <  1:739794/140‑1 (MQ=255)                                                                                                                                gAATACACCGCCCGTTTCAGGAAGTGCGCCACCAACCTTTCAGTACGAAAATTGCTTTCATTGTCGATACCCTTTTTACGTGAACTTGCGTACTAGTTAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAata   <  2:58576/140‑1 (MQ=255)                                                                                                                                 aaTACACCGCCCGTTTCAGGAAGTGCGCCACCAACCTTTCAGTACGAAAATTGCTTTCATTGTCGATACCCTTTTTACGTGAACTTGCGTACTAGTTAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAatat  >  2:1004110/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                     GATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATTGTTATTCTCTAATTTTGTTCAAAAAAAAGCCCGCTCATTAGGCGGGCTGGGTATCTGATTGCTTTACGCATGGTGAATACACTGCCCGTTTCAGGAAGTGCGCCACCAACCTTTCAGTACGAAAATTGCTTTCATTGTCGATACCCTTTTTACGTGAACTTGCGTACTAGTTAACTAGTTCGATGATTAATTGTCAACAGCTCATTTCAGAATAT  >  NC_000913/1322895‑1323162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑