Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,543,407	T→C	V218V (GTA→GTG)	narU ←	nitrate/nitrite transporter NarU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,543,407	0	T	C	100.0%	173.9 / NA	49	V218V (GTA→GTG)	narU	nitrate/nitrite transporter NarU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/19);  total (30/19)

TGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCT  >  NC_000913/1543271‑1543539                                                                                                                                         |                                                                                                                                     tGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGCAcc                                                                                                                                   <  1:742818/140‑1 (MQ=255)   gCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGCACCCa                                                                                                                                 >  2:330466/1‑140 (MQ=255)   gCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGCACCCa                                                                                                                                 >  1:752455/1‑140 (MQ=255)              cAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCgg                                                                                                                      >  1:767768/1‑140 (MQ=255)                  cTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCAtt                                                                                                                  >  1:233275/1‑140 (MQ=255)                           cAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGa                                                                                                         <  2:752455/140‑1 (MQ=255)                                 tGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCAc                                                                                                   >  2:97842/1‑140 (MQ=255)                                    aGGCGTTGTAAGACAGGGAGCTGGTCGGCGATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGa                                                                                                         <  1:211255/131‑1 (MQ=255)                                    aGGCGTTGTAAGACAGGGAGCTGGTCGGCGATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGa                                                                                                         >  2:211255/1‑131 (MQ=255)                                        gTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCg                                                                                            <  2:233275/140‑1 (MQ=255)                                        gTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCg                                                                                            <  1:330466/140‑1 (MQ=255)                                             aaGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGc                                                                                       <  1:15261/140‑1 (MQ=255)                                                    ggAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGt                                                                                >  1:328176/1‑140 (MQ=255)                                                        cTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGc                                                                            >  2:312170/1‑140 (MQ=255)                                                          ggTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCa                                                                          >  1:914673/1‑140 (MQ=255)                                                            tCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCAtt                                                                        >  1:938115/1‑140 (MQ=255)                                                              ggCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTg                                                                                    >  2:1044923/1‑126 (MQ=255)                                                              ggCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTg                                                                                    <  1:1044923/126‑1 (MQ=255)                                                                      aGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCgaga                                                              <  2:767768/140‑1 (MQ=255)                                                                      aGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCgaga                                                              <  1:872861/140‑1 (MQ=255)                                                                                            attcatCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACaaaaa                                        >  2:258983/1‑140 (MQ=255)                                                                                                   ccTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAg                                 <  1:258983/140‑1 (MQ=255)                                                                                                      gACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCgg                              >  1:488390/1‑140 (MQ=255)                                                                                                      gACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCgg                              >  1:769041/1‑140 (MQ=255)                                                                                                           tGCGGCGATCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCg                               <  2:93227/134‑1 (MQ=255)                                                                                                           tGCGGCGATCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCg                               >  1:93227/1‑134 (MQ=255)                                                                                                           tGCGGCGATCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCaa                         >  1:791061/1‑140 (MQ=255)                                                                                                           tGCGGCGATCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCaa                         >  2:890731/1‑140 (MQ=255)                                                                                                            gcggcgATCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAAc                        >  2:385632/1‑140 (MQ=255)                                                                                                            gcggcgATCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAAc                        >  2:444807/1‑140 (MQ=255)                                                                                                            gcggcgATCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAAc                        >  2:556215/1‑140 (MQ=255)                                                                                                            gcggcgATCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAAc                        >  1:47634/1‑140 (MQ=255)                                                                                                            gcggcgATCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAAc                        >  1:289800/1‑140 (MQ=255)                                                                                                            gcggcgATCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAAc                        >  2:176001/1‑140 (MQ=255)                                                                                                            gcggcgATCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAAc                        >  1:219400/1‑140 (MQ=255)                                                                                                            gcggcgATCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAAc                        >  1:1003657/1‑140 (MQ=255)                                                                                                            gcggcgATCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAAc                        >  1:165904/1‑140 (MQ=255)                                                                                                                cgATCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGc                    <  2:47634/140‑1 (MQ=255)                                                                                                                  aTCGTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTg                  >  1:378013/1‑140 (MQ=255)                                                                                                                     gTGGCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCAt               <  2:378013/140‑1 (MQ=255)                                                                                                                       ggCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAg                     >  2:243452/1‑132 (MQ=255)                                                                                                                       ggCAATCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAg                     <  1:243452/132‑1 (MQ=255)                                                                                                                          aaTCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCAttacac          <  2:1003657/140‑1 (MQ=255)                                                                                                                          aaTCGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCAttacac          <  1:176001/140‑1 (MQ=255)                                                                                                                             cGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCAttacactta       >  1:864204/1‑140 (MQ=255)                                                                                                                             cGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCAttacactta       <  2:165904/140‑1 (MQ=255)                                                                                                                             cGCCAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCAttacactta       >  1:1034949/1‑140 (MQ=255)                                                                                                                                cAGTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCAttacacttacac    <  1:385632/140‑1 (MQ=255)                                                                                                                                  gTAGCGGCACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCt  <  1:556215/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     TGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCT  >  NC_000913/1543271‑1543539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑