Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,567,363	A→G	intergenic (‑144/+141)	yddY ← / ← yddW	protein YddY/putative lipoprotein YddW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,567,363	0	A	G	100.0%	241.9 / NA	67	intergenic (‑144/+141)	yddY/yddW	protein YddY/putative lipoprotein YddW
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/33);  total (34/33)

TGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAAT  >  NC_000913/1567227‑1567497                                                                                                                                         |                                                                                                                                       tGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCAt                                                                                                                                     >  2:50713/1‑140 (MQ=255) tGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCAt                                                                                                                                     <  2:251749/140‑1 (MQ=255)   aaaTATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCAttt                                                                                                                                   >  2:920008/1‑140 (MQ=255)   aaaTATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCAttt                                                                                                                                   <  1:905796/140‑1 (MQ=255)     atatGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCAttttt                                                                                                                                 >  1:432398/1‑140 (MQ=255)        tGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTttatt                                                                                                                              >  1:856104/1‑140 (MQ=255)                 aCTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTa                                                                                                                     >  2:33128/1‑140 (MQ=255)                    gAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATgg                                                                                                                  <  2:179676/140‑1 (MQ=255)                    gAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATgg                                                                                                                  <  1:808162/140‑1 (MQ=255)                    gAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATgg                                                                                                                  <  2:580768/140‑1 (MQ=255)                      aaaGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCt                                                                                                                <  2:826820/140‑1 (MQ=255)                      aaaGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCt                                                                                                                >  1:311411/1‑140 (MQ=255)                       aaGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTg                                                                                                               >  2:1007276/1‑140 (MQ=255)                       aaGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTg                                                                                                               >  2:913117/1‑140 (MQ=255)                            aTAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTaa                                                                                                          >  2:883586/1‑140 (MQ=255)                              aaGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACt                                                                                                        <  1:560845/140‑1 (MQ=255)                               aGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTa                                                                                                       >  2:99849/1‑140 (MQ=255)                                          aCAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTaa                                                                                            <  2:311411/140‑1 (MQ=255)                                           cAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAAt                                                                                           <  1:1007276/140‑1 (MQ=255)                                                tCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGAc                                                                                      <  1:626108/140‑1 (MQ=255)                                                        cGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTtaagtaa                                                                              >  2:463539/1‑140 (MQ=255)                                                        cGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTtaagtaa                                                                              >  1:11568/1‑140 (MQ=255)                                                        cGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTtaagtaa                                                                              >  2:310064/1‑140 (MQ=255)                                                        cGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTtaagtaa                                                                              >  1:577761/1‑140 (MQ=255)                                                         gCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTtaagtaag                                                                             <  2:577761/140‑1 (MQ=255)                                                          cTACTTTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTAGTAGGGATTTATGGCTAATTAACTAAGTGTGGTTAATTTGACTTAACTAAGc                                                                            <  2:822382/140‑1 (MQ=255)                                                          cTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGc                                                                            <  1:630479/140‑1 (MQ=255)                                                          cTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGc                                                                            <  1:463539/140‑1 (MQ=255)                                                          cTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGc                                                                            <  2:3670/140‑1 (MQ=255)                                                          cTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGc                                                                            <  1:920008/140‑1 (MQ=255)                                                          cTACTGTCCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGc                                                                            <  1:558452/140‑1 (MQ=255)                                                                  cttcAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGattat                                                                    >  1:548274/1‑140 (MQ=255)                                                                    tcAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGAttatta                                                                  <  1:422369/140‑1 (MQ=255)                                                                     cAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTtaa                                                                                  >  1:1076158/1‑123 (MQ=255)                                                                     cAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTtaa                                                                                  <  2:1076158/123‑1 (MQ=255)                                                                          gagTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTTATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGa                                                            >  1:730298/1‑140 (MQ=255)                                                                          gagTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGa                                                            <  1:33128/140‑1 (MQ=255)                                                                          gagTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGa                                                            <  2:114405/140‑1 (MQ=255)                                                                          gagTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGa                                                            <  2:11568/140‑1 (MQ=255)                                                                          gagTATAGCCCATCGTAATTATGTTTCGTTGACAGCTAATATCGTATTGTTGTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGa                                                            <  2:476545/140‑1 (MQ=255)                                                                             tataGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAg                                                         >  2:681287/1‑140 (MQ=255)                                                                               taGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGtt                                                       >  1:724568/1‑140 (MQ=255)                                                                                      tCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAgg                                                >  1:347915/1‑140 (MQ=255)                                                                                       cGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAggg                                               >  2:352059/1‑140 (MQ=255)                                                                                       cGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAggg                                               >  2:65230/1‑140 (MQ=255)                                                                                       cGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAggg                                               >  2:349039/1‑140 (MQ=255)                                                                                       cGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAggg                                               >  1:948434/1‑140 (MQ=255)                                                                                       cGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAggg                                               >  2:253429/1‑140 (MQ=255)                                                                                       cGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAggg                                               >  1:1012326/1‑140 (MQ=255)                                                                                       cGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAggg                                               >  1:1045741/1‑140 (MQ=255)                                                                                       cGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAggg                                               >  2:131465/1‑140 (MQ=255)                                                                                           attattTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAAc                                           >  1:741581/1‑140 (MQ=255)                                                                                           attattTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAAc                                           >  1:270056/1‑140 (MQ=255)                                                                                              attTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAg                                        <  1:253429/140‑1 (MQ=255)                                                                                                 tttCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCg                                     <  1:681287/140‑1 (MQ=255)                                                                                                 tttCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCg                                     <  1:50713/140‑1 (MQ=255)                                                                                                 tttCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCg                                     <  2:548274/140‑1 (MQ=255)                                                                                                 tttCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCg                                     <  1:401899/140‑1 (MQ=255)                                                                                                       tGACAGCGAATATCGTATGGTTTTTCATCTTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGt                               <  2:730298/140‑1 (MQ=255)                                                                                                              gAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCAc                        <  2:923371/140‑1 (MQ=255)                                                                                                              gAATATCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCAc                        <  1:310064/140‑1 (MQ=255)                                                                                                                atatCGTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCg                      >  1:924697/1‑140 (MQ=255)                                                                                                                     gTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTg                 >  1:1073431/1‑140 (MQ=255)                                                                                                                     gTATGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTg                 >  1:246041/1‑140 (MQ=255)                                                                                                                        tGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTa              <  2:347915/140‑1 (MQ=255)                                                                                                                        tGGTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTa              <  1:352059/140‑1 (MQ=255)                                                                                                                         ggTTTTTCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTAc             <  2:724568/140‑1 (MQ=255)                                                                                                                             tttCATATTCATGCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAg         >  1:112199/1‑140 (MQ=255)                                                                                                                              ttcatattcatgCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGt        >  2:534/1‑140 (MQ=255)                                                                                                                                    ttcatgCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGACTTGTTACAAAGTAAGAAt  <  2:420793/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       TGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAAT  >  NC_000913/1567227‑1567497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑