Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,634,642	C→T	G82S (GGC→AGC)	tfaQ ←	Qin prophage; putative tail fiber assembly protein TfaQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,634,642	0	C	T	100.0%	117.1 / NA	52	G82S (GGC→AGC)	tfaQ	Qin prophage; putative tail fiber assembly protein TfaQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/30);  total (22/30)

TCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGAC  >  NC_000913/1634510‑1634776                                                                                                                                     |                                                                                                                                       tCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGcc                                                                                                                                 <  2:832989/140‑1 (MQ=18) tCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGcc                                                                                                                                 <  1:504714/140‑1 (MQ=18)  ccGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCa                                                                                                                                <  2:153832/140‑1 (MQ=18)     gAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGt                                                                                                                             >  2:1066611/1‑140 (MQ=17)           tttttCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATaaa                                                                                                                       <  1:1066611/140‑1 (MQ=18)                     tCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTAcc                                                                                                             >  2:134300/1‑140 (MQ=17)                      ccGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTAccc                                                                                                            <  1:514013/140‑1 (MQ=18)                              ttCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATc                                                                                                    <  2:2280/140‑1 (MQ=18)                                cACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCtt                                                                                                  >  1:616946/1‑140 (MQ=17)                                cACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCAACGTCATAAACCGTTTTAc                                                                                                              <  1:225226/128‑1 (MQ=16)                                cACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCAACGTCATAAACCGTTTTAc                                                                                                              >  2:225226/1‑128 (MQ=17)                                   ccAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCgat                                                                                                        >  1:820094/1‑131 (MQ=17)                                   ccAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCgat                                                                                                        <  2:820094/131‑1 (MQ=18)                                      ggCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACg                                                                                             >  1:412285/1‑139 (MQ=17)                                      ggCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACg                                                                                             <  2:412285/139‑1 (MQ=17)                                       gCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTGACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTAccc                                                                                                            >  1:41203/1‑123 (MQ=17)                                       gCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTGACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTAccc                                                                                                            <  2:41203/123‑1 (MQ=16)                                           tgCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCaa                                                                                               <  2:20877/132‑1 (MQ=17)                                           tgCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCaa                                                                                               >  1:20877/1‑132 (MQ=17)                                           tgCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGc                                                                                       >  2:552527/1‑140 (MQ=17)                                           tgCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGc                                                                                       >  1:121211/1‑140 (MQ=17)                                           tgCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGc                                                                                       >  1:616765/1‑140 (MQ=17)                                                ttCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGa                                                                                  <  1:328837/140‑1 (MQ=17)                                                 tCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGAt                                                                                 >  1:498278/1‑140 (MQ=17)                                                   cACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGa                                                                               <  1:134300/140‑1 (MQ=17)                                                        cTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACtcat                                                                          <  1:260573/140‑1 (MQ=17)                                                        cTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACtcat                                                                          <  2:860012/140‑1 (MQ=17)                                                        cTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACtcat                                                                          <  1:552527/140‑1 (MQ=17)                                                        cTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACtcat                                                                          <  2:200691/140‑1 (MQ=17)                                                          gAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACtcatca                                                                        >  1:807953/1‑140 (MQ=17)                                                                ccccTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTg                                                                  >  2:66590/1‑140 (MQ=17)                                                                      ccGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACa                                                            <  2:616765/140‑1 (MQ=17)                                                                            aTAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACg                                                      <  2:524586/140‑1 (MQ=17)                                                                                ccAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAagc                                                  >  1:689741/1‑140 (MQ=17)                                                                                ccAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAagc                                                  <  1:900181/140‑1 (MQ=17)                                                                                    gTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAagccagc                                              >  1:926215/1‑140 (MQ=17)                                                                                      aaCATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCg                                            <  2:616946/140‑1 (MQ=17)                                                                                             tCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATc                                     >  1:278160/1‑140 (MQ=17)                                                                                                  tAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCg                                >  2:522827/1‑140 (MQ=18)                                                                                                    aCGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGt                              <  2:926215/140‑1 (MQ=17)                                                                                                      ggACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGc                            <  2:278160/140‑1 (MQ=17)                                                                                                      ggACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGc                            <  2:689741/140‑1 (MQ=17)                                                                                                      ggACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGc                            <  2:807953/140‑1 (MQ=17)                                                                                                            aGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGc                            >  2:183968/1‑134 (MQ=18)                                                                                                            aGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGc                            <  2:707995/134‑1 (MQ=17)                                                                                                            aGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGc                            <  1:183968/134‑1 (MQ=17)                                                                                                            aGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGc                            >  1:707995/1‑134 (MQ=18)                                                                                                             gTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCg                     >  2:869064/1‑140 (MQ=18)                                                                                                             gTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCg                     <  1:762635/140‑1 (MQ=17)                                                                                                                        aataaCGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGc                            >  2:678035/1‑122 (MQ=18)                                                                                                                        aataaCGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGc                            <  1:678035/122‑1 (MQ=17)                                                                                                                                gTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGAc  <  2:861354/140‑1 (MQ=17)                                                                                                                                     |                                                                                                                                       TCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGAC  >  NC_000913/1634510‑1634776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑