Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	194,885	T→C	intergenic (+168/‑18)	dxr → / → ispU	1‑deoxy‑D‑xylulose 5‑phosphate reductoisomerase/undecaprenyl diphosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	194,885	0	T	C	100.0%	210.7 / NA	58	intergenic (+168/‑18)	dxr/ispU	1‑deoxy‑D‑xylulose 5‑phosphate reductoisomerase/undecaprenyl diphosphate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/24);  total (34/24)

GGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTG  >  NC_000913/194759‑195020                                                                                                                               |                                                                                                                                        gggCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATaaaaa                                                                                                                            >  1:307991/1‑140 (MQ=255)     ttCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAAcgc                                                                                                                        <  2:307991/140‑1 (MQ=255)            atatAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTACAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGAtgt                                                                                                                 <  1:760224/140‑1 (MQ=255)             tataGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGAtgtt                                                                                                                <  1:721883/140‑1 (MQ=255)                      cgcCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTAc                                                                                                       >  2:856620/1‑140 (MQ=255)                      cgcCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTAc                                                                                                       >  1:328380/1‑140 (MQ=255)                      cgcCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGATAc                                                                                                       >  1:214683/1‑140 (MQ=255)                       gcCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGAtgtt                                                                                                                <  2:971440/130‑1 (MQ=255)                       gcCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGAtgtt                                                                                                                >  1:971440/1‑130 (MQ=255)                           tCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAAc                                                                                                  >  2:781418/1‑140 (MQ=255)                                   tAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGATACTCAACCACTTAGc                                                                                          <  2:214683/140‑1 (MQ=255)                                     agtagtTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGa                                                                                        >  2:617289/1‑140 (MQ=255)                                     agtagtTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGa                                                                                        >  1:738738/1‑140 (MQ=255)                                     agtagtTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGa                                                                                        >  1:569020/1‑140 (MQ=255)                                            ggCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTg                                                                                 >  2:195925/1‑140 (MQ=255)                                              cTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGcc                                                                               >  2:337131/1‑140 (MQ=255)                                              cTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGcc                                                                               >  1:541609/1‑140 (MQ=255)                                              cTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGcc                                                                               <  1:617289/140‑1 (MQ=255)                                                      ggTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATg                                                                       <  2:180625/140‑1 (MQ=255)                                                       gTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATgg                                                                      >  1:604163/1‑140 (MQ=255)                                                        tCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGc                                                                     <  2:541609/140‑1 (MQ=255)                                                        tCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGc                                                                     >  2:567110/1‑140 (MQ=255)                                                        tCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGc                                                                     <  1:337131/140‑1 (MQ=255)                                                          aGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTg                                                                   <  2:478346/140‑1 (MQ=255)                                                          aGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTg                                                                   <  1:781418/140‑1 (MQ=255)                                                             tatGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCg                                                                <  1:195925/140‑1 (MQ=255)                                                                 ccGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCAt                                                            >  2:896593/1‑140 (MQ=255)                                                                      gTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGc                                                       <  2:787043/140‑1 (MQ=255)                                                                      gTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGc                                                       >  1:84437/1‑140 (MQ=255)                                                                      gTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGc                                                       <  1:896593/140‑1 (MQ=255)                                                                      gTTTTACACGGCTTTTTTTTGTATAGGCTTCAATATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGc                                                       >  1:357648/1‑140 (MQ=255)                                                                       ttttACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCg                                                      <  2:84437/140‑1 (MQ=255)                                                                        tttACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGa                                                     <  2:981042/140‑1 (MQ=255)                                                                                 cTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATgg                                            >  1:946233/1‑140 (MQ=255)                                                                                           tataGGCTTCAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATgg                                  >  2:106796/1‑140 (MQ=255)                                                                                           tataGGCTTCAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATgg                                  >  1:459415/1‑140 (MQ=255)                                                                                                gCTTCAGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCt                             >  2:353953/1‑140 (MQ=255)                                                                                                     aGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGc                              <  1:1091717/134‑1 (MQ=255)                                                                                                     aGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGc                              >  2:1091717/1‑134 (MQ=255)                                                                                                     aGTATTCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCT‑CTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCaa                       >  1:789695/1‑140 (MQ=255)                                                                                                          tCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCaaaaaa                   >  1:253472/1‑140 (MQ=255)                                                                                                          tCCTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCaaaaaa                   >  1:439995/1‑140 (MQ=255)                                                                                                            cTGAGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGc                 <  1:78160/140‑1 (MQ=255)                                                                                                               aGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAg                  <  2:589159/136‑1 (MQ=255)                                                                                                               aGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAg                  >  1:589159/1‑136 (MQ=255)                                                                                                               aGTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAg              >  1:255845/1‑140 (MQ=255)                                                                                                                gTACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAgg             <  2:253472/140‑1 (MQ=255)                                                                                                                 tACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAggg            >  1:190928/1‑140 (MQ=255)                                                                                                                 tACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGACAATGGCCGCTGGGCAAAAAAGc                 >  2:872534/1‑135 (MQ=255)                                                                                                                 tACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGACAATGGCCGCTGGGCAAAAAAGc                 <  1:872534/135‑1 (MQ=255)                                                                                                                 tACCGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATAATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAggg            >  2:519455/1‑140 (MQ=255)                                                                                                                    cGTAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAg         >  1:11425/1‑140 (MQ=255)                                                                                                                      tAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGAt       >  1:1070100/1‑140 (MQ=255)                                                                                                                      tAAACCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGAt       >  1:1016362/1‑140 (MQ=255)                                                                                                                        aaCCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTc     <  2:459415/140‑1 (MQ=255)                                                                                                                         aCCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGc                 >  1:390083/1‑127 (MQ=255)                                                                                                                         aCCCTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGc                 <  2:390083/127‑1 (MQ=255)                                                                                                                           ccTGCCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTg  <  2:328380/140‑1 (MQ=255)                                                                                                                               |                                                                                                                                        GGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCTGTCAGGGAATAAAAAACGCGTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTG  >  NC_000913/194759‑195020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑