Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,008,322	G→C	A16P (GCC→CCC)	yedE →	putative selenium transporter YedE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,008,322	0	G	C	100.0%	187.8 / NA	53	A16P (GCC→CCC)	yedE	putative selenium transporter YedE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (24/29);  total (24/29)

TATTTTTGTGAATTATTACCCCGAAAATAAATTTGCATGGTGTATGATTCGCGACTCTTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGG‑CACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCT  >  NC_000913/2008185‑2008456                                                                                                                                          |                                                                                                                                        tattTTTGTGAATTATTACCCCGAAAATAAATTTGCATGGTGTATGATTCGCGACTCTTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGccc                                                                                                                                       >  2:226828/1‑140 (MQ=255)   tttttGTGAATTATTACCCCGAAAATAAATTTGCATGGTGTATGATTCGCGACTCTTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCcccc                                                                                                                                     <  2:918758/140‑1 (MQ=255)        gtgAATTATTACCCCGAAAATAAATTTGCATGGTGTATGATTCGCGACTCTTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATcc                                                                                                                                >  1:1014492/1‑140 (MQ=255)                      cGAAAATAAATTTGCATGGTGTATGATTCGCGACTCTTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATcgcg                                                                                                                  >  2:489193/1‑140 (MQ=255)                        aaaaTAAATTTGCATGGTGTATGATTCGCGACTCTTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCgcgg                                                                                                                 <  2:732258/139‑1 (MQ=255)                        aaaaTAAATTTGCATGGTGTATGATTCGCGACTCTTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCgcgg                                                                                                                 >  1:732258/1‑139 (MQ=255)                            taaatTTGCATGGTGTATGATTCGCGACTCTTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGt                                                                                                            <  1:1019670/140‑1 (MQ=255)                            taaatTTGCATGGTGTATGATTCGCGACTCTTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGt                                                                                                            <  1:226828/140‑1 (MQ=255)                              aatTTGCATGGTGTATGATTCGCGACTCTTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTAt                                                                                                          <  2:988416/140‑1 (MQ=255)                                 ttGCATGGTGTATGATTCGCGACTCTTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATtct                                                                                                       <  2:63668/140‑1 (MQ=255)                                       ggTGTATGATTCGCGACTCTTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTAtt                                                                                                         >  1:798458/1‑132 (MQ=255)                                       ggTGTATGATTCGCGACTCTTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTAtt                                                                                                         <  2:798458/132‑1 (MQ=255)                                         tgtATGATTCGCGACTCTTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCtactt                                                                                               >  1:668776/1‑140 (MQ=255)                                          gtATGATTCGCGACTCTTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCtactta                                                                                              <  2:954102/140‑1 (MQ=255)                                          gtATGATTCGCGACTCTTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCtactta                                                                                              <  2:668776/140‑1 (MQ=255)                                             tGATTCGCGACTCTTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGGATTTTCTCtacttactt                                                                                           >  2:299826/1‑139 (MQ=255)                                                  cgcgACTCTTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGGATTTTCTCTACTTACTAttttt                                                                                      >  2:596636/1‑139 (MQ=255)                                                   gcgACTCTTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTAtttttg                                                                                     >  2:64706/1‑138 (MQ=255)                                                   gcgACTCTTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTgg                                                                                     >  1:375942/1‑140 (MQ=255)                                                   gcgACTCTTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTgg                                                                                     >  1:862947/1‑140 (MQ=255)                                                   gcgACTCTTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTgg                                                                                     >  2:898379/1‑140 (MQ=255)                                                   gcgACTCTTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGGATTCTCTCTACTTACTATTTTgg                                                                                     >  2:628483/1‑140 (MQ=255)                                                     gACTCTTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCa                                                                                   >  1:361382/1‑140 (MQ=255)                                                        tctTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTa                                                                                <  2:361382/140‑1 (MQ=255)                                                        tctTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGGATTCTCTCTACTTACTATTTTTGCAttt                                                                                >  2:531144/1‑139 (MQ=255)                                                           tGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTg                                                                             <  1:248904/140‑1 (MQ=255)                                                           tGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTg                                                                             <  1:308942/140‑1 (MQ=255)                                                             tttCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGG‑c                                                                          <  1:299826/140‑1 (MQ=255)                                                             tttCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGG‑c                                                                          <  1:898379/140‑1 (MQ=255)                                                                  aaCCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTTTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGG‑CACCtt                                                                     >  1:306257/1‑140 (MQ=255)                                                                       tCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGG‑CACCTTTTggg                                                                <  1:628483/140‑1 (MQ=255)                                                                             ttCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGG‑CACCTTTTGGGCTGTcc                                                          >  2:90738/1‑139 (MQ=255)                                                                             ttCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGGATTTTTTTTTCTTTCTATTTTTGCATTTCTGGCCCCCTTTTgggtggtc                                                           >  2:308420/1‑135 (MQ=255)                                                                             ttCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGGAATTTCTCTCCTTAAACTTTTTGGATTTCTCG‑CACCCTTTTGGCtcgca                                                          >  2:545369/1‑136 (MQ=255)                                                                                agagGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGG‑CACCTTTTGGGCTGTCACgg                                                       <  2:862947/140‑1 (MQ=255)                                                                                  agGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGG‑CACCTTTTGGGCTGTCACGGGt                                                     <  2:375942/140‑1 (MQ=255)                                                                                       aCATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTTGCATTACTGG‑CACCTTTTggg                                                                >  1:513112/1‑124 (MQ=255)                                                                                       aCATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGG‑CACCTTTTggg                                                                <  2:513112/124‑1 (MQ=255)                                                                                        cATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGG‑CACCTTTTGGGCTGTCACGGGTGAAttt                                               <  1:593866/140‑1 (MQ=255)                                                                                              tgtcatgGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTTGCATTACTGG‑CACCCTTTTGGCTGTCACGGGTGAATTTAcccct                                         >  2:1005297/1‑138 (MQ=255)                                                                                                         aaaTTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGG‑CACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCa                              <  1:545369/139‑1 (MQ=255)                                                                                                           aTTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGG‑CACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGc                            <  1:531144/140‑1 (MQ=255)                                                                                                           aTTCAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGG‑CACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGc                            <  2:1014492/140‑1 (MQ=255)                                                                                                              cAAACACGCCTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGG‑CACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTcc                         <  1:596636/140‑1 (MQ=255)                                                                                                                      ccTGGTTGATTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTTCTTACTATTTTGGCCTTACTGG‑CACCTTTTGGGCTGTCACGGGTGGATTTACCCGTTGGGGCGGCCAGCTCCTGCaaatt                 >  2:574277/1‑137 (MQ=255)                                                                                                                              aTTAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGG‑CACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGt         <  1:308420/140‑1 (MQ=255)                                                                                                                               ttAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGG‑CACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGt         >  1:437095/1‑139 (MQ=255)                                                                                                                               ttAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGG‑CACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGt         <  2:437095/139‑1 (MQ=255)                                                                                                                                tAAATTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGG‑CACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTcc       <  2:325876/140‑1 (MQ=255)                                                                                                                                   aTTCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTTCTTACTATTTTGGCATTACTGG‑CACCTTTTGGGCTGTCACGGGGGAATTTACCCCTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCCTg    >  2:196975/1‑140 (MQ=255)                                                                                                                                    ttCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGG‑CACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGc   >  1:676569/1‑140 (MQ=255)                                                                                                                                     tttGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGG‑CACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCt  <  2:306257/138‑1 (MQ=255)                                                                                                                                     tCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTTCTATTTTGGCATTACTGG‑CACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCt  <  1:312615/140‑1 (MQ=255)                                                                                                                                     tCTGGCCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGG‑CACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCt  <  2:17751/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        TATTTTTGTGAATTATTACCCCGAAAATAAATTTGCATGGTGTATGATTCGCGACTCTTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGG‑CACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCT  >  NC_000913/2008185‑2008456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑