Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	199,200	A→G	S425G (AGC→GGC)	bamA →	outer membrane protein assembly factor BamA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	199,200	0	A	G	100.0%	308.8 / NA	86	S425G (AGC→GGC)	bamA	outer membrane protein assembly factor BamA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (48/38);  total (48/38)

GATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCAAAAACGATTACCAGACCT  >  NC_000913/199065‑199329                                                                                                                                        |                                                                                                                                  gATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCtt                                                                                                                               <  2:948869/140‑1 (MQ=255)  aTCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTc                                                                                                                              <  1:850832/140‑1 (MQ=255)  aTCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTc                                                                                                                              >  2:420216/1‑140 (MQ=255)            tCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTa                                                                                                                    <  2:8837/140‑1 (MQ=255)             cAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTAt                                                                                                                   >  1:468140/1‑140 (MQ=255)             cAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTAt                                                                                                                   >  1:163762/1‑140 (MQ=255)                 gTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGt                                                                                                               <  2:468140/140‑1 (MQ=255)                    aGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTAc                                                                                                            <  2:1074199/140‑1 (MQ=255)                    aGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTAc                                                                                                            <  1:420216/140‑1 (MQ=255)                     ggAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACg                                                                                                           >  2:302569/1‑140 (MQ=255)                     ggAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACg                                                                                                           >  2:419755/1‑140 (MQ=255)                     ggAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACg                                                                                                           >  2:328715/1‑140 (MQ=255)                      gAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACgg                                                                                                          <  1:302569/140‑1 (MQ=255)                          gTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACt                                                                                                      >  1:659341/1‑140 (MQ=255)                          gTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACt                                                                                                      >  2:594524/1‑140 (MQ=255)                          gTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACt                                                                                                      >  2:932518/1‑140 (MQ=255)                          gTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACt                                                                                                      >  2:944451/1‑140 (MQ=255)                          gTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACt                                                                                                      >  1:1009666/1‑140 (MQ=255)                           tCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTa                                                                                                             >  2:1041380/1‑132 (MQ=255)                           tCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTa                                                                                                             <  1:1041380/132‑1 (MQ=255)                                   gTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGc                                                                                             >  2:97287/1‑140 (MQ=255)                                    tCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCg                                                                                            >  2:196177/1‑140 (MQ=255)                                    tCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCg                                                                                            >  1:295677/1‑140 (MQ=255)                                      tGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTg                                                                                          <  1:173905/140‑1 (MQ=255)                                              tttGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCa                                                                                  >  2:896306/1‑140 (MQ=255)                                                tGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAgg                                                                                <  2:295677/140‑1 (MQ=255)                                                   aaCTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTg                                                                             <  1:944451/140‑1 (MQ=255)                                                       gTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGgtgt                                                                         >  2:773380/1‑140 (MQ=255)                                                       gTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGgtgt                                                                         >  2:125651/1‑140 (MQ=255)                                                       gTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGgtg                                                                          >  1:429329/1‑139 (MQ=255)                                                       gTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGgtg                                                                          <  2:429329/139‑1 (MQ=255)                                                        tCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGtgtg                                                                        >  1:837932/1‑140 (MQ=255)                                                            taccgataccCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTgcagc                                                                    <  1:479162/140‑1 (MQ=255)                                                                 ataccCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGAt                                                               <  1:52781/140‑1 (MQ=255)                                                                 ataccCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGAt                                                               >  2:632877/1‑140 (MQ=255)                                                                       aaCGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTgg                                                         >  2:123358/1‑140 (MQ=255)                                                                        aCGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGt                                                        <  1:196177/140‑1 (MQ=255)                                                                        aCGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGt                                                        <  1:896306/140‑1 (MQ=255)                                                                        aCGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGt                                                        <  2:1009666/140‑1 (MQ=255)                                                                        aCGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGCCTGGTGTGCAGCACCATAACCGGt                                                        <  2:465062/140‑1 (MQ=255)                                                                         cGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGtt                                                       >  2:305724/1‑140 (MQ=255)                                                                         cGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGtt                                                       >  2:310473/1‑140 (MQ=255)                                                                         cGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGtt                                                       <  1:123358/140‑1 (MQ=255)                                                                         cGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGtt                                                       >  1:1073321/1‑140 (MQ=255)                                                                         cGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGtt                                                       >  1:902161/1‑140 (MQ=255)                                                                            gtTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAgg                                                    >  1:380614/1‑140 (MQ=255)                                                                             ttCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTgg                                                         >  1:410469/1‑134 (MQ=255)                                                                             ttCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTgg                                                         <  2:410469/134‑1 (MQ=255)                                                                             ttCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGt                                                   >  2:877758/1‑140 (MQ=255)                                                                             ttCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGt                                                   <  1:305724/140‑1 (MQ=255)                                                                             ttCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGt                                                   <  1:310473/140‑1 (MQ=255)                                                                             ttCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGt                                                   >  2:869881/1‑140 (MQ=255)                                                                                  ggTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAgg                                              >  1:710293/1‑140 (MQ=255)                                                                                  ggTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAgg                                              >  2:910723/1‑140 (MQ=255)                                                                                     aGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTa                                           >  1:722976/1‑140 (MQ=255)                                                                                     aGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTa                                           <  1:594524/140‑1 (MQ=255)                                                                                      gCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTAt                                          >  1:795532/1‑140 (MQ=255)                                                                                       cccGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTgg                                                         <  2:446768/124‑1 (MQ=255)                                                                                       cccGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTgg                                                         >  1:446768/1‑124 (MQ=255)                                                                                          ggACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTg                                      <  2:756831/140‑1 (MQ=255)                                                                                           gACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGt                                     <  1:125651/140‑1 (MQ=255)                                                                                           gACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGt                                     <  1:773380/140‑1 (MQ=255)                                                                                            aCCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGtt                                    >  2:498551/1‑140 (MQ=255)                                                                                            aCCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGtt                                    >  2:750925/1‑140 (MQ=255)                                                                                                 gTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTAt                               >  1:730587/1‑140 (MQ=255)                                                                                                      tGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACg                          >  2:821846/1‑140 (MQ=255)                                                                                                      tGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACg                          >  2:615166/1‑140 (MQ=255)                                                                                                      tGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACg                          >  1:555559/1‑140 (MQ=255)                                                                                                        tcgtcTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACggg                        <  1:877758/140‑1 (MQ=255)                                                                                                           tcTACAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGAcc                     >  2:392555/1‑140 (MQ=255)                                                                                                               cAAGGTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCaaaa                 <  1:615166/140‑1 (MQ=255)                                                                                                                  ggTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCAAAAACg              <  1:498551/140‑1 (MQ=255)                                                                                                                  ggTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCAAAAACg              <  1:750925/140‑1 (MQ=255)                                                                                                                  ggTAAAAGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCAAAAACg              <  1:821846/140‑1 (MQ=255)                                                                                                                       aaGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCAAAAACGATTAc         <  2:795532/140‑1 (MQ=255)                                                                                                                       aaGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCAAAAACGATTAc         <  2:464513/140‑1 (MQ=255)                                                                                                                       aaGAGCGCAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCAAAAACGATTAc         <  2:385770/140‑1 (MQ=255)                                                                                                                            cgcAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCAAAAACGATTACCAGAc    <  1:97287/140‑1 (MQ=255)                                                                                                                            cgcAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCAAAAACGATTACCAGAc    >  2:18049/1‑140 (MQ=255)                                                                                                                            cgcAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCAAAAACGATTACCAGAc    >  1:948928/1‑140 (MQ=255)                                                                                                                            cgcAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCAAAAACGATTACCAGAc    >  1:1094078/1‑140 (MQ=255)                                                                                                                              cAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCAAAAACGATTACCAGACCt  <  2:710293/140‑1 (MQ=255)                                                                                                                              cAACACCGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCAAAAACGATTACCAGACCt  >  1:650582/1‑140 (MQ=255)                                                                                                                                    cGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCAAAAACGATTACCAGAc    <  2:431666/132‑1 (MQ=255)                                                                                                                                    cGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCAAAAACGATTACCAGAc    >  2:931031/1‑132 (MQ=255)                                                                                                                                    cGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCAAAAACGATTACCAGAc    <  1:931031/132‑1 (MQ=255)                                                                                                                                    cGGTGGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCAAAAACGATTACCAGAc    >  1:431666/1‑132 (MQ=255)                                                                                                                                        |                                                                                                                                  GATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACGTGTTCCGGGTAGCCCGGACCAGGTTGATGTCGTCTACAAGGTAAAAGAGCGCAACACCGGTAGCTTCAACTTTGGTATTGGTTACGGTACTGAAAGTGGCGTGAGCTTCCAGGCTGGTGTGCAGCAGGATAACTGGTTAGGTACAGGTTATGCTGTTGGTATCAACGGGACCAAAAACGATTACCAGACCT  >  NC_000913/199065‑199329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑