Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,188,193	T→C	G74G (GGA→GGG)	yehA ←	putative fimbrial adhesin YehA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,188,193	0	T	C	100.0%	202.7 / NA	56	G74G (GGA→GGG)	yehA	putative fimbrial adhesin YehA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/27);  total (29/27)

CTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCTGAAC  >  NC_000913/2188057‑2188315                                                                                                                                         |                                                                                                                           cTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCca                                                                                                                         <  2:411614/140‑1 (MQ=255) cTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCca                                                                                                                         <  1:242751/140‑1 (MQ=255)               gCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGa                                                                                                           <  2:1016337/140‑1 (MQ=255)               gCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGa                                                                                                           >  2:1005688/1‑140 (MQ=255)               gCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGa                                                                                                           <  2:929732/140‑1 (MQ=255)               gCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGa                                                                                                           >  1:929732/1‑140 (MQ=255)                  gCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACgg                                                                                                        <  2:667932/140‑1 (MQ=255)                    aGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCcaa                                                                                                                        <  1:1004751/122‑1 (MQ=255)                    aGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCcaa                                                                                                                        >  2:1004751/1‑122 (MQ=255)                    aGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGc                                                                                                      >  2:189748/1‑140 (MQ=255)                         tCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATa                                                                                                 >  1:722479/1‑140 (MQ=255)                             cGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAgggg                                                                                             <  1:925991/140‑1 (MQ=255)                              gTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGt                                                                                            <  2:722479/140‑1 (MQ=255)                              gTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGt                                                                                            <  2:213806/140‑1 (MQ=255)                                 aCTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAGGCCCAACAATAATATCCGACgg                                                                                                        >  2:239102/1‑125 (MQ=255)                                 aCTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAGGCCCAACAATAATATCCGACgg                                                                                                        <  1:239102/125‑1 (MQ=255)                                      aTAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTAt                                                                                    <  2:651523/140‑1 (MQ=255)                                       tAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTAtt                                                                                   >  2:808087/1‑140 (MQ=255)                                            aCTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTAc                                                                              >  2:928141/1‑140 (MQ=255)                                             cTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGa                                                                                          >  2:479670/1‑127 (MQ=255)                                             cTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGa                                                                                          <  1:479670/127‑1 (MQ=255)                                               ggTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTa                                                                           >  1:741926/1‑140 (MQ=255)                                               ggTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGCATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTa                                                                           >  2:312721/1‑140 (MQ=255)                                                   aaaCTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCa                                                                       >  2:632320/1‑140 (MQ=255)                                                     aCTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATg                                                                     >  1:985156/1‑140 (MQ=255)                                                     aCTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATg                                                                     >  1:750884/1‑140 (MQ=255)                                                     aCTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATg                                                                     >  2:79920/1‑140 (MQ=255)                                                        ggTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACg                                                                  >  1:509200/1‑140 (MQ=255)                                                          tCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTAttt                                                                                  >  2:1038415/1‑122 (MQ=255)                                                          tCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTAttt                                                                                  <  1:1038415/122‑1 (MQ=255)                                                          tCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGaa                                                                >  1:211747/1‑140 (MQ=255)                                                          tCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGaa                                                                >  2:500112/1‑140 (MQ=255)                                                          tCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGaa                                                                >  2:676109/1‑140 (MQ=255)                                                              attattTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCAt                                                            >  1:173018/1‑140 (MQ=255)                                                              attattTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCAt                                                            <  2:173018/140‑1 (MQ=255)                                                                   ttGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTaaaa                                                       <  1:16161/140‑1 (MQ=255)                                                                   ttGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTaaaa                                                       >  1:161422/1‑140 (MQ=255)                                                                   ttGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTaaaa                                                       >  2:917312/1‑140 (MQ=255)                                                                     gACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAAtt                                                     <  2:161422/140‑1 (MQ=255)                                                                          cGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCt                                                <  1:107578/140‑1 (MQ=255)                                                                           gTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTc                                               <  1:1005688/140‑1 (MQ=255)                                                                                           tGGTCCATTCAAAATGTAAATTTAATTTTATGGCATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTtgt                               <  1:312721/140‑1 (MQ=255)                                                                                              tCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGtt                            >  1:575903/1‑140 (MQ=255)                                                                                              tCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGtt                            >  1:20667/1‑140 (MQ=255)                                                                                                  ttCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAg                        >  1:640463/1‑140 (MQ=255)                                                                                                   tCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGt                       <  2:509200/140‑1 (MQ=255)                                                                                                    cAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTa                      <  2:750884/140‑1 (MQ=255)                                                                                                    cAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTa                      <  2:985156/140‑1 (MQ=255)                                                                                                             aatttaatttTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCaaaa             >  1:550060/1‑140 (MQ=255)                                                                                                             aatttaatttTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCaaaa             >  1:550059/1‑140 (MQ=255)                                                                                                                 taatttTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATccc         <  2:211747/140‑1 (MQ=255)                                                                                                                  aatttTATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATcccc        <  1:189748/140‑1 (MQ=255)                                                                                                                     tttATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCTg     <  2:1023557/140‑1 (MQ=255)                                                                                                                     tttATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCTg     <  1:632320/140‑1 (MQ=255)                                                                                                                       tATGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCTGaa   <  1:917312/140‑1 (MQ=255)                                                                                                                        aTGGTATCGTTATAAAGCCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCTGaac  >  2:549293/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                           CTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCTGAAC  >  NC_000913/2188057‑2188315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑