Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,274,432	T→C	V85A (GTG→GCG)	yejB →	putative oligopeptide ABC transporter membrane subunit YejB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,274,432	0	T	C	100.0%	157.2 / NA	45	V85A (GTG→GCG)	yejB	putative oligopeptide ABC transporter membrane subunit YejB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/22);  total (23/22)

CGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTAA  >  NC_000913/2274295‑2274567                                                                                                                                          |                                                                                                                                        cgccgcCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGCGa                                                                                                                                       >  1:376890/1‑140 (MQ=255)      ccATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGCGATCGCt                                                                                                                                  >  1:741323/1‑140 (MQ=255)                  gTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCAt                                                                                                                      <  1:609099/140‑1 (MQ=255)                      tGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGCGATCg                                                                                                                                    <  2:261338/122‑1 (MQ=255)                      tGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGCGATCg                                                                                                                                    >  1:261338/1‑122 (MQ=255)                      tGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCt                                                                                                                  <  2:376890/140‑1 (MQ=255)                       gCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCTa                                                                                                                 <  1:806365/140‑1 (MQ=255)                       gCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCTa                                                                                                                 <  2:741323/140‑1 (MQ=255)                               aTTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTg                                                                                                         <  2:1064215/140‑1 (MQ=255)                                      ggCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGcc                                                                                                  >  2:745679/1‑140 (MQ=255)                                       gcgcAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCg                                                                                                 <  2:650577/140‑1 (MQ=255)                                       gcgcAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCg                                                                                                 <  2:413805/140‑1 (MQ=255)                                        cgcAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGcc                                                                                                  >  2:624723/1‑138 (MQ=255)                                        cgcAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGcc                                                                                                  <  1:624723/138‑1 (MQ=255)                                         gcAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGAt                                                                                               <  1:745679/140‑1 (MQ=255)                                           aGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATcc                                                                                             >  2:317473/1‑140 (MQ=255)                                              cGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGc                                                                                                   >  1:366512/1‑131 (MQ=255)                                              cGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGc                                                                                                   <  2:366512/131‑1 (MQ=255)                                                          tCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCa                                                                              >  1:281864/1‑140 (MQ=255)                                                           cGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCaa                                                                             <  1:999659/140‑1 (MQ=255)                                                                cagccaTGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGt                                                                                     >  2:161218/1‑127 (MQ=255)                                                                cagccaTGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGt                                                                                     <  1:161218/127‑1 (MQ=255)                                                                 agccaTGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGct                                                                       <  1:780132/140‑1 (MQ=255)                                                                   ccaTGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGctct                                                                     <  1:622215/140‑1 (MQ=255)                                                                   ccaTGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGctct                                                                     <  1:317473/140‑1 (MQ=255)                                                                       gcgcAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGa                                                                 <  2:457039/140‑1 (MQ=255)                                                                             aCGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACAt                                                           >  2:96379/1‑140 (MQ=255)                                                                                       gCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGAt                                                 >  1:708726/1‑140 (MQ=255)                                                                                                 cGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATa                                       >  2:11870/1‑140 (MQ=255)                                                                                                     aGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCt                                   >  2:140542/1‑140 (MQ=255)                                                                                                              cGTGGCGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAg                          >  1:829369/1‑140 (MQ=255)                                                                                                               gTGGCGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAgc                         >  1:290198/1‑140 (MQ=255)                                                                                                                tGGCGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAgcg                        >  2:676244/1‑140 (MQ=255)                                                                                                                tGGCGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAgcg                        <  1:140542/140‑1 (MQ=255)                                                                                                                 ggCGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAgcgc                       >  1:77551/1‑140 (MQ=255)                                                                                                                 ggCGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAgcgc                       >  1:728942/1‑140 (MQ=255)                                                                                                                 ggCGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAgcgc                       >  1:297358/1‑140 (MQ=255)                                                                                                                 ggCGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAgcgc                       >  2:735894/1‑140 (MQ=255)                                                                                                                  gCGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGTAGCGcc                      >  1:712549/1‑140 (MQ=255)                                                                                                                   cGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCt                     >  2:233557/1‑140 (MQ=255)                                                                                                                   cGGACGCGGATTAGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCt                     <  2:77551/140‑1 (MQ=255)                                                                                                                               aGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGTAGCGCCTCGGTGCTGACGc         <  2:712549/140‑1 (MQ=255)                                                                                                                               aGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGc         <  2:14854/140‑1 (MQ=255)                                                                                                                               aGATCCAGAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGc         <  1:233557/140‑1 (MQ=255)                                                                                                                                      gAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTaa  >  1:1016289/1‑140 (MQ=255)                                                                                                                                      gAAGCGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTaa  >  1:385175/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        CGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTAA  >  NC_000913/2274295‑2274567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑