Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,339,970	T→C	V135A (GTC→GCC)	ubiG →	bifunctional 3‑demethylubiquinone‑8 3‑O‑methyltransferase and 2‑octaprenyl‑6‑hydroxyphenol methylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,339,970	0	T	C	100.0%	154.1 / NA	43	V135A (GTC→GCC)	ubiG	bifunctional 3‑demethylubiquinone‑8 3‑O‑methyltransferase and 2‑octaprenyl‑6‑hydroxyphenol methylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/27);  total (16/27)

CCATTGCAGGTGGCAAAACTGCACGCACTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCATG  >  NC_000913/2339834‑2340106                                                                                                                                         |                                                                                                                                         ccATTGCAGGTGGCAAAACTGCACGCACTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGcccc                                                                                                                                       <  1:1052853/140‑1 (MQ=255)       cAGGTGGCAAAACTGCACGCACTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGCCCCCGATcc                                                                                                                                 <  1:1072077/140‑1 (MQ=255)         ggTGGCAAAACTGCACGCACTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGCCCCCGATCCGc                                                                                                                               >  2:463291/1‑140 (MQ=255)              cAAAACTGCACGCACTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGTGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGCCCCCGATCCGcagtca                                                                                                                          <  1:963087/140‑1 (MQ=255)              cAAAACTGCACGCACTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGCCCCCGATCCGcagtca                                                                                                                          <  1:653839/140‑1 (MQ=255)              cAAAACTGCACGCACTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGCCCCCGATCCGcagtca                                                                                                                          <  1:1059913/140‑1 (MQ=255)              cAAAACTGCACGCACTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGCCCCCGATCCGcagtca                                                                                                                          >  2:1059913/1‑140 (MQ=255)               aaaaCTGCACGCACTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGCCCCCGATCCGcagtcag                                                                                                                         <  1:952/140‑1 (MQ=255)                   cTGCACGCACTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGCCCCCGATCCGCAGTCAGTGGt                                                                                                                     <  1:510121/140‑1 (MQ=255)                      cacgcacTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGCCCCCGATCCGCAGTCAGTGGTCag                                                                                                                  >  1:1058314/1‑140 (MQ=255)                        cgcacTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGCCCCCGATCCGCAGTCAGTGGTCagag                                                                                                                <  1:46102/140‑1 (MQ=255)                         gcacTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGCCCCCGATCCGCAGTCAGTGGTCAGAGc                                                                                                               <  2:95352/140‑1 (MQ=255)                                   gCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGCCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCaa                                                                                                     <  2:385880/140‑1 (MQ=255)                                     ggCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGCCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACt                                                                                                   >  2:369989/1‑140 (MQ=255)                                     ggCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTACATGGAGATGCTGGAGCACGCCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACt                                                                                                   >  2:160547/1‑140 (MQ=255)                                               tGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGCCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCa                                                                                         <  1:463291/140‑1 (MQ=255)                                                    tACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGCCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCaa                                                                                                     <  1:508391/123‑1 (MQ=255)                                                    tACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGCCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCaa                                                                                                     >  2:508391/1‑123 (MQ=255)                                                      cGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGCCCCCGATCCGCAGTCAGTTGTCAGAGCCTGTGCGCAACTGGTGAAACCAGgcggcg                                                                                  <  2:967870/140‑1 (MQ=255)                                                      cGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGCCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGgcggcg                                                                                  >  1:626345/1‑140 (MQ=255)                                                        ggCAGGAACCCGTGGAAGACCACGCGGC‑AAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGCCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATg                                                                               <  2:271527/139‑1 (MQ=255)                                                                       aaGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGCCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGaca                                                                 >  1:1003688/1‑140 (MQ=255)                                                                              cgcgGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGCCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAAcc                                                          >  1:755178/1‑140 (MQ=255)                                                                              cgcgGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGCCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAAcc                                                          >  1:172377/1‑140 (MQ=255)                                                                                              gggCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGCCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATg                                          <  2:766951/140‑1 (MQ=255)                                                                                               ggCAGTATGATGTGGTGACCTACATGGAGATGCTGGAGCACGCCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATgg                                         <  1:160547/140‑1 (MQ=255)                                                                                                   gTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGCCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGa                                     <  2:755178/140‑1 (MQ=255)                                                                                                            ggtgACCTGCATGGAGATGCTGGAGCACGCCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCt                                       <  2:955265/129‑1 (MQ=255)                                                                                                            ggtgACCTGCATGGAGATGCTGGAGCACGCCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCt                                       >  1:955265/1‑129 (MQ=255)                                                                                                             gtgACCTGCATGGAGATGCTGGAGCACGCCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATg                                   >  1:193523/1‑132 (MQ=255)                                                                                                             gtgACCTGCATGGAGATGCTGGAGCACGCCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATg                                   <  2:193523/132‑1 (MQ=255)                                                                                                             gtgACCTGCATGGAGATGCTGGAGCACGCCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCggtggt                           >  2:487085/1‑140 (MQ=255)                                                                                                                aCCTGCATGGAGATGCTGGAGCACGCCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGtggttg                         <  2:247998/139‑1 (MQ=255)                                                                                                                aCCTGCATGGAGATGCTGGAGCACGCCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGtggttg                         >  1:247998/1‑139 (MQ=255)                                                                                                                 ccTGCATGGAGATGCTGGAGCACGCCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGtggttggt                       <  2:920122/140‑1 (MQ=255)                                                                                                                   tGCATGGAGATGCTGGAGCACGCCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGc                     <  1:503637/140‑1 (MQ=255)                                                                                                                          agaTGCTGGAGCACGCCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAatat              >  1:1045778/1‑140 (MQ=255)                                                                                                                                  gAGCACGCCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGaa                 <  2:166510/129‑1 (MQ=255)                                                                                                                                  gAGCACGCCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGaa                 >  1:166510/1‑129 (MQ=255)                                                                                                                                   aGCACGCCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTgcgc     <  1:487085/140‑1 (MQ=255)                                                                                                                                     cACGCCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCAt   <  2:1058314/140‑1 (MQ=255)                                                                                                                                     cACGCCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCAt   <  2:1045778/140‑1 (MQ=255)                                                                                                                                      aCGCCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCATg  <  1:369989/140‑1 (MQ=255)                                                                                                                                      aCGCCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCATg  <  1:677830/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CCATTGCAGGTGGCAAAACTGCACGCACTGGAAAGCGGCATTCAGGTGGATTACGTGCAGGAAACCGTGGAAGAGCACGCGGCAAAACATGCCGGGCAGTATGATGTGGTGACCTGCATGGAGATGCTGGAGCACGTCCCCGATCCGCAGTCAGTGGTCAGAGCCTGTGCGCAACTGGTGAAACCAGGCGGCGATGTCTTTTTCTCGACACTTAACCGCAACGGCAAGTCATGGCTGATGGCGGTGGTTGGTGCGGAATATATTTTGCGCATG  >  NC_000913/2339834‑2340106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑