Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,592,616	T→C	V337A (GTG→GCG)	dapE →	succinyl‑diaminopimelate desuccinylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,592,616	0	T	C	100.0%	165.0 / NA	46	V337A (GTG→GCG)	dapE	succinyl‑diaminopimelate desuccinylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/27);  total (19/27)

TTTGACCGCGCGCGGTAAACTGGTGGATGCGGTCGTTAACGCGGTTGAGCACTATAATGAAATTAAACCGCAGCTACTGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGTGGTGGAACTCGGGCCGGTCAATGCCACTATTCATAAAATTAATGAATGTGTGAACGCTGCCGACCTGCAGCTACTTGCCCGTATGTATCAACGTATCATGGAACAGCTCGTCGCCTGATGAG  >  NC_000913/2592485‑2592738                                                                                                                                    |                                                                                                                           tttGTCCGCGCGCGGTAAACTGGTGGATGCGGTCGTTAACGCGGTTGAGCACTATAATGAAATTAAACCGCAGCTACTGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAAc                                                                                                                    <  2:1006640/140‑1 (MQ=255)      ccGCGCGCGGTAAACTGGTGGATGCGGTCGTTAACGCGGTTGAGCACTATAATGAAATTAAACCGCAGCTACTGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCggg                                                                                                               <  2:418077/140‑1 (MQ=255)       cgcgcgcgGTAAACTGGTGGATGCGGTCGTTAACGCGGTTGAGCACTATAATGAAATTAAACCGCAGCTACTGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGc                                                                                                              <  2:195795/140‑1 (MQ=255)          gcgcgGTAAACTGGTGGATGCGGTCGTTAACGCGGTTGAGCACTATAATGAAATTAAACCGCAGCTACTGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGgcggtg                                                                                                                        >  2:964039/1‑127 (MQ=255)          gcgcgGTAAACTGGTGGATGCGGTCGTTAACGCGGTTGAGCACTATAATGAAATTAAACCGCAGCTACTGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGgcggtg                                                                                                                        <  1:964039/127‑1 (MQ=255)          gcgcgGTAAACTGGTGGATGCGGTCGTTAACGCGGTTGAGCACTATAATGAAATTAAACCGCAGCTACTGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCgg                                                                                                           >  1:480538/1‑140 (MQ=255)                          gATGCGGTCGTTAACGCGGTTGAGCACTATAATGAAATTAAACCGCAGCTACTGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCa                                                                                           >  2:329719/1‑140 (MQ=255)                          gATGCGGTCGTTAACGCGGTTGAGCACTATAATGAAATTAAACCGCAGCTACTGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCa                                                                                           >  2:1089125/1‑140 (MQ=255)                            tGCGGTCGTTAACGCGGTTGAGCACTATAATGAAATTAAACCGCAGCTACTGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCAc                                                                                                 <  1:55770/132‑1 (MQ=255)                            tGCGGTCGTTAACGCGGTTGAGCACTATAATGAAATTAAACCGCAGCTACTGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCAc                                                                                                 >  2:55770/1‑132 (MQ=255)                            tGCGGTCGTTAACGCGGTTGAGCACTATAATGAAATTAAACCGCAGCTACTGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATa                                                                                         >  2:343069/1‑140 (MQ=255)                            tGCGGTCGTTAACGCGGTTGAGCACTATAATGAAATTAAACCGCAGCTACTGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATa                                                                                         <  2:1057969/140‑1 (MQ=255)                              cGGTCGTTAACGCGGTTGAGCACTATAATGAAATTAAACCGCAGCTACTGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATaaa                                                                                       <  1:152948/140‑1 (MQ=255)                                 tCGTTAACGCGGTTGAGCACTATAATGAAATTAAACCGCAGCTACTGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATAAAAtt                                                                                    >  1:834219/1‑140 (MQ=255)                                   gTTAACGCGGTTGAGCACTATAATGAAATTAAACCGCAGCTACTGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATAAAATTaa                                                                                  <  2:798483/140‑1 (MQ=255)                                    ttAACGCGGTTGAGCACTATAATGAAATTAAACCGCAGCTACTGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCa                                                                                           >  1:257669/1‑130 (MQ=255)                                    ttAACGCGGTTGAGCACTATAATGAAATTAAACCGCAGCTACTGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCa                                                                                           <  2:257669/130‑1 (MQ=255)                                    ttAACGCGGTTGAGCACTATAATGAAATTAAACCGCAGCTACTGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATAAAATTaat                                                                                 <  2:549280/140‑1 (MQ=255)                                    ttAACGCGGTTGAGCACTATAATGAAATTAAACCGCAGCTACTGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATAAAATTaat                                                                                 <  2:411617/140‑1 (MQ=255)                                    ttAACGCGGTTGAGCACTATAATGAAATTAAACCGCAGCTACTGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATAAAATTaat                                                                                 >  2:368857/1‑140 (MQ=255)                                      aaCGCGGTTGAGCACTATAATGAAATTAAACCGCAGCTACTGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATAAAATTaatga                                                                               <  2:308192/140‑1 (MQ=255)                                           ggTTGAGCACTATAATGAAATTAAACCGCAGCTACTGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATAAAATTAATGAAtgtg                                                                          >  2:95876/1‑140 (MQ=255)                                                       taATGAAATTAAACCGCAGCTACTGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATAAAATTAATGAATGTGTGAACGCTGCCg                                                              >  2:187176/1‑140 (MQ=255)                                                        aaTGAAATTAAACCGCAGCTACTGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATAAAATTAATGAATGTGTGAACGCTGCCGa                                                             <  2:318957/140‑1 (MQ=255)                                                             aaTTAAACCGCAGCTACTGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATAAAATTAATGAATGTGTGAACGCTGCCGACCTGc                                                        <  2:241733/140‑1 (MQ=255)                                                               ttAAACCGCAGCTACTGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATAAAATTAATGAATGTGTGAACGCTGCCGACCTGCAg                                                      >  2:819534/1‑140 (MQ=255)                                                               ttAAACCGCAGCTACTGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATAAAATTAATGAATGTGTGAACGCTGCCGACCTGCAg                                                      <  1:814844/140‑1 (MQ=255)                                                                 aaaCCGCAGCTACTGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATAATATTAATGAATGTGTGAACGCTGCCGACCTGCAGCt                                                    <  1:187176/140‑1 (MQ=255)                                                                 aaaCCGCAGCTACTGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATAAAATTAATGAATGTGTGAACGCTGCCGACCTGCAGCt                                                    >  2:151551/1‑140 (MQ=255)                                                                 aaaCCGCAGCTACTGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATAAAATTAATGAATGTGTGAACGCTGCCGACCTGCAGCt                                                    >  2:417813/1‑140 (MQ=255)                                                                   aCCGCAGCTACTGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATAAAATTAATGAATGTGTGAACGCTGCCGACCTGCAGCTAc                                                  <  1:329719/140‑1 (MQ=255)                                                                       cAGCTACTGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATAAAATTAATGAATGTGTGAACGCTGCCGACCTGCAGCTACTTGc                                              <  2:541730/140‑1 (MQ=255)                                                                           tACTGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATAAAATTAATGAATGTGTGAACGCTGCCGACCTGCAGCTACTTGCCCgt                                          <  2:480538/140‑1 (MQ=255)                                                                              tGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATAAAATTAATGAATGTGTGAACGCTGCCGACCTGCAGCTACTTGCCCgtatg                                       <  1:95876/140‑1 (MQ=255)                                                                                 ccACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATAAAATTAATGAATGTGTGAACGCTGCCGACCTGCAGCTACTTGCCCgtatgtat                                    >  2:149662/1‑140 (MQ=255)                                                                                      ggCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATAAAATTAATGAATGTGTGAACGCTGCCGACCTGCAGCTACTTGCCCGTATGTATCAACg                               <  1:149662/140‑1 (MQ=255)                                                                                      ggCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATAAAATTAATGAATGTGTGAACGCTGCCGACCTGCAGCTACTTGCCCGTATGTATCAACg                               <  2:834219/140‑1 (MQ=255)                                                                                       gCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATAAAATTAATGAATGTGTGAACGCTGCCGACCTGCAGCTACTTGCCCGTATGTATCAACGt                              >  2:316894/1‑140 (MQ=255)                                                                                       gCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATAAAATTAATGAATGTGTGAACGCTGCCGACCTGCAGCTACTTGCCCGTATGTATCAACGt                              >  2:1029817/1‑140 (MQ=255)                                                                                       gCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATAAAATTAATGAATGTGTGAACGCTGCCGACCTGCAGCTACTTGCCCGTATGTATCAACGt                              >  2:647333/1‑140 (MQ=255)                                                                                                 cGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATAAAATTAATGAATGTGTGAACGCTGCCGACCTGCAGCTACTTGCCCGTATGTATCAACGTATCATGGAAc                    <  1:760176/140‑1 (MQ=255)                                                                                                        cgcTTTATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATAAAATTAATGAATGTGTGAACGCTGCCGACCTGCAGCTACTTGCCCGTATGTATCAACGTATCATGGAACAGCtcgt             <  1:1089125/140‑1 (MQ=255)                                                                                                            ttATTGCCCGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATAAAATTAATGAATGTGTGAACGCTGCCGACCTGCAGCTACTTGCCCGTATGTATCAACGTATCATGGAACAGCTCGTCGcc         <  1:819534/140‑1 (MQ=255)                                                                                                                  cccGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATAAAATTAATGAATGTGTGAACGCTGCCGACCTGCAGCTACTTGCCCGTATGTATCAACGTATCATGGAACAGCTCGTCGCCtgatga   <  1:316894/140‑1 (MQ=255)                                                                                                                   ccGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATAAAATTAATGAATGTGTGAACGCTGCCGACCTGCAGCTACTTGCCCGTATGTATCAACGTATCATGGAACAGCTCGTCGCCTGATGAg  >  2:18807/1‑140 (MQ=255)                                                                                                                   ccGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATAAAATTAATGAATGTGTGAACGCTGCCGACCTGCAGCTACTTGCCCGTATGTATCAACGTATCATGGAACAGCTCGTCGCCTGATGAg  <  1:343069/140‑1 (MQ=255)                                                                                                                   ccGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATAAAATTAATGAATGTGTGAACGCTGCCGACCTGCAGCTACTTGCCCGTATGTATCAACGTATCATGGAACAGCTCGTCGCCTGATGAg  >  2:938615/1‑140 (MQ=255)                                                                                                                   ccGCATGGGGGCGCAGGCGGTGGAACTCGGGCCGGTCAATGCCACTATTCATAAAATTAATGAATGTGTGAACGCTGCCGACCTGCAGCTACTTGCCCGTATGTATCAACGTATCATGGAACAGCTCGTCGCCTGATGAg  <  1:151551/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                           TTTGACCGCGCGCGGTAAACTGGTGGATGCGGTCGTTAACGCGGTTGAGCACTATAATGAAATTAAACCGCAGCTACTGACCACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGTGGTGGAACTCGGGCCGGTCAATGCCACTATTCATAAAATTAATGAATGTGTGAACGCTGCCGACCTGCAGCTACTTGCCCGTATGTATCAACGTATCATGGAACAGCTCGTCGCCTGATGAG  >  NC_000913/2592485‑2592738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑