| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,630,822 | T→C | V166A (GTG→GCG) | yfgI → | nalidixic acid resistance protein YfgI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,630,822 | 0 | T | C | 98.0% | 180.7 / ‑3.8 | 51 | coding (497/540 nt) | yfgI | nalidixic acid resistance protein YfgI |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (20/30); minor base . (0/1); total (20/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAGCCCGCGTTAAGCGAGAAAACGAATTTATCGATCAGGAACTGAAGCACAAAGCTGCGCAAACCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAAT > NC_000913/2630687‑2630958 | aagCCCGCGTTAAGCGAGAAAACGAATTTATCGATCAGGAACTGAAGCACAAAGCTGCGCAAACCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGc > 2:464635/1‑140 (MQ=255) agCCCGCGTTAAGCGAGAAAACGAATTTATCGATCAGGAACTGAAGCACAAAGCTGCGCAAACCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCa < 2:175954/140‑1 (MQ=255) ccGCGTTAAGCGAGAAAACGAATTTATCGATCAGGAACTGAAGCACAAAGCTGCGCAAACCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAg < 2:775523/140‑1 (MQ=255) gcgTTAAGCGAGAAAACGAATTTATCGATCAGGAACTGAAGCACAAAGCTGCGCAAACCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCa < 2:714132/140‑1 (MQ=255) cgTTAAGCGAGAAAACGAATTTATCGATCAGGAACTGAAGCACAAAGCTGCGCAAACCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCag > 1:140222/1‑140 (MQ=255) aGCGAGAAAACGAATTTATCGATCAGGAACTGAAGCACAAAGCTGCGCAAACCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCag > 2:488033/1‑135 (MQ=255) aGCGAGAAAACGAATTTATCGATCAGGAACTGAAGCACAAAGCTGCGCAAACCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCag < 1:488033/135‑1 (MQ=255) aaaaCGAATTTATCGATCAGGAACTGAAGCACAAAGCTGCGCAAACCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACaaa < 2:778934/140‑1 (MQ=255) aaaaCGAATTTATCGATCAGGAACTGAAGCACAAAGCTGCGCAAACCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACaaa > 1:24955/1‑140 (MQ=255) aTTTATCGATCAGGAACTGAAGCACAAAGCTGCGCAAACCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACa < 1:464635/140‑1 (MQ=255) tttATCGATCAGGAACTGAAGCACAAAGCTGCGCAAACCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAg < 1:58201/140‑1 (MQ=255) tATCGATCAGGAACTGAAGCACAAAGCTGCGCAAACCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCt > 1:576611/1‑140 (MQ=255) cGATCAGGAACTGAAGCACAAAGCTGCGCAAACCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGt < 1:977400/140‑1 (MQ=255) gATCAGGAACTGAAGCACAAAGCTGCGCAAACCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGtt < 2:64900/140‑1 (MQ=255) aCTGAAGCACAAAGCTGCGCAAACCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTtaattaat > 2:1032139/1‑140 (MQ=255) aCTGAAGCACAAAGCTGCGCAAACCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTtaattaat < 1:599423/140‑1 (MQ=255) aCTGAAGCACAAAGCTGCGCAAACCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTtaattaat < 2:607751/140‑1 (MQ=255) aaGCACAAAGCTGCGCAAACCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGAt > 1:645884/1‑140 (MQ=255) aaGCACAAAGCTGCGCAAACCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGAt > 2:374142/1‑140 (MQ=255) aaGCACAAAGCTGCGCAAACCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGAt > 2:490152/1‑140 (MQ=255) caAAGCTGCGCAAACCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAg > 2:418731/1‑140 (MQ=255) caAAGCTGCGCAAACCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAg > 1:429897/1‑140 (MQ=255) aaaGCTGCGCAAACCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGt < 2:576611/140‑1 (MQ=255) aaaGCTGCGCAAACCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGt < 1:987299/140‑1 (MQ=255) aaaGCTGCGCAAACCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGt < 1:490152/140‑1 (MQ=255) aGCTGCGCAAACCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTaa > 2:197836/1‑140 (MQ=255) gcAAACCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCaa < 1:197836/140‑1 (MQ=255) tgtgGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAAt > 1:688175/1‑140 (MQ=255) gtgCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCtt > 2:420475/1‑140 (MQ=255) tgCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACtt < 2:202473/123‑1 (MQ=255) tgCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACtt > 1:202473/1‑123 (MQ=255) tCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGAt > 1:8441/1‑140 (MQ=255) gACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAAccc < 1:418731/140‑1 (MQ=255) gACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAAccc < 1:897251/140‑1 (MQ=255) aCGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCct < 2:429897/140‑1 (MQ=255) cGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCctc < 2:645884/140‑1 (MQ=255) cGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCctc < 1:1032139/140‑1 (MQ=255) aCTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTg > 1:746626/1‑126 (MQ=255) aCTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTg < 2:746626/126‑1 (MQ=255) cTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGtt > 1:562245/1‑140 (MQ=255) cTGAAGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGtt > 1:52513/1‑140 (MQ=255) aaGGCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTg > 2:1003931/1‑140 (MQ=255) ggCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGtt < 1:678742/140‑1 (MQ=255) gCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGttc < 2:688175/140‑1 (MQ=255) gCGGTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGttc < 1:1003931/140‑1 (MQ=255) ggTCGCGATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGttcat < 1:374142/140‑1 (MQ=255) cgcgATCTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGttcattca < 2:707567/140‑1 (MQ=255) tCTGATGAAAAGCG‑CGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGc < 2:422385/140‑1 (MQ=255) cTGATGAAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGc < 2:583550/140‑1 (MQ=255) gaAAAGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGt < 2:8441/140‑1 (MQ=255) aaGCGCGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCaa < 1:651382/140‑1 (MQ=255) gcgcGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAAt < 1:420475/140‑1 (MQ=255) | AAGCCCGCGTTAAGCGAGAAAACGAATTTATCGATCAGGAACTGAAGCACAAAGCTGCGCAAACCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAAT > NC_000913/2630687‑2630958 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |