Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,644,674	A→G	V64A (GTT→GCT)	ndk ←	nucleoside diphosphate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,644,674	0	A	G	100.0%	166.4 / NA	47	V64A (GTT→GCT)	ndk	nucleoside diphosphate kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/20);  total (27/20)

CAGCGTAATCAGCGCGCAGAGTACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTGCAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGA  >  NC_000913/2644540‑2644809                                                                                                                                       |                                                                                                                                        cAGCGTAATCAGCGCGCAGAGTACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAGCCAGa                                                                                                                                    <  1:420469/140‑1 (MQ=255)     gTAATCAGCGCGCAGAGTACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAGCCAGACCAt                                                                                                                                <  1:922064/140‑1 (MQ=255)        aTCAGCGCGCAGAGTACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAGCCAGACCATCaa                                                                                                                             >  2:129287/1‑140 (MQ=255)             cgcgcAGAGTACCAGCCAGTGCGTTTGTCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAGCCAGACCATCAAAGAAc                                                                                                                        >  1:1010686/1‑140 (MQ=255)              gcgcAGAGTACCAGCCAGTGCGTTTGTCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAGCCAGACCATCAAAGAACg                                                                                                                       <  2:1010686/140‑1 (MQ=255)                gcAGAGTACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAGCCAGACCATCAAAGAACGGt                                                                                                                     >  2:878731/1‑140 (MQ=255)                  agagTACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAGCCAGACCATCAAAGAACg                                                                                                                       <  1:672870/136‑1 (MQ=255)                  agagTACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAGCCAGACCATCAAAGAACg                                                                                                                       >  2:672870/1‑136 (MQ=255)                  agagTACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAGCCAGACCATCAAAGAACGGttt                                                                                                                   >  1:789808/1‑140 (MQ=255)                   gagTACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAGCCAGACCATCAAAGAACGGtttt                                                                                                                  <  1:878731/140‑1 (MQ=255)                        ccagccagTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAGCCAGACCATCAAAGAACGGTTTTCCATc                                                                                                             <  1:822802/140‑1 (MQ=255)                              agTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAGCCAGACCATCAAAGaa                                                                                                                         <  2:886562/122‑1 (MQ=255)                              agTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAGCCAGACCATCAAAGaa                                                                                                                         >  1:886562/1‑122 (MQ=255)                                tGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAGCCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAg                                                                                                     >  1:394851/1‑140 (MQ=255)                                tGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAGCCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAg                                                                                                     >  2:1066198/1‑140 (MQ=255)                                tGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAGCCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAg                                                                                                     >  1:991810/1‑140 (MQ=255)                                           gATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAGCCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCa                                                                                          <  1:129287/140‑1 (MQ=255)                                                gTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAGCCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGc                                                                                     >  2:24210/1‑140 (MQ=255)                                                             gATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAGCCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTc                                                                        <  2:789808/140‑1 (MQ=255)                                                              aTCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAGCCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCa                                                                       >  1:91751/1‑140 (MQ=255)                                                                cgcgGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAGCCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAgg                                                                     >  2:42655/1‑140 (MQ=255)                                                                cgcgGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAGCCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAgg                                                                     >  1:776215/1‑140 (MQ=255)                                                                 gcgGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAGCCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGt                                                                    <  2:979195/140‑1 (MQ=255)                                                                 gcgGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAGCCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGt                                                                    <  2:941237/140‑1 (MQ=255)                                                                        cGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAGCCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTGCAGCAt                                                             >  2:496976/1‑140 (MQ=255)                                                                         gCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAGCCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTGCAGCAtt                                                            >  2:107679/1‑140 (MQ=255)                                                                           tGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAGCCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTGCAGCAtttt                                                          >  1:655151/1‑140 (MQ=255)                                                                             aaCGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAGCCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTGCAGCATTTTgg                                                        >  2:116970/1‑140 (MQ=255)                                                                                  cGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAGCCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTGCAGCATTTTGGTGCca                                                   >  2:771070/1‑140 (MQ=255)                                                                                              ccAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAGCCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTGCAGCATTTTGGTGCCAACAATTTTGAAc                                       <  2:91751/140‑1 (MQ=255)                                                                                                    cGGAAACCACGATCGGGCCAGAGGTCATGAATTCAGCCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTGCAGCATTTTGGTGCCAACAATTTTGAACCCTGCa                                 <  1:116970/140‑1 (MQ=255)                                                                                                           cACGATCGGGCCAGAGGTCATGAATTCAGCCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTGCAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCaa                          >  1:405461/1‑140 (MQ=255)                                                                                                             cGATCGGGCCAGAGGTCATGAATTCAGCCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTGCAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAg                        <  1:24210/140‑1 (MQ=255)                                                                                                               aTCGGGCCAGAGGTCATGAATTCAGCCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTGCAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAgcg                      <  2:655151/140‑1 (MQ=255)                                                                                                                   ggCCAGAGGTCATGAATTCAGCCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTGCAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCa                  <  1:107679/140‑1 (MQ=255)                                                                                                                   ggCCAGAGGTCATGAATTCAGCCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTGCAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCa                  >  1:255411/1‑140 (MQ=255)                                                                                                                    gCCAGAGGTCATGAATTCAGCCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTGCAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCaa                 >  1:966045/1‑140 (MQ=255)                                                                                                                             cATGAATTCAGCCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTGCAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAgcgcg                    >  2:875748/1‑128 (MQ=255)                                                                                                                             cATGAATTCAGCCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTGCAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAgcgcg                    <  1:875748/128‑1 (MQ=255)                                                                                                                              aTGAATTCAGCCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTGCAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAg               <  1:391050/132‑1 (MQ=255)                                                                                                                              aTGAATTCAGCCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTGCAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAg               >  2:391050/1‑132 (MQ=255)                                                                                                                              aTGAATTCAGCCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTGCAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTAcc       >  2:513919/1‑140 (MQ=255)                                                                                                                              aTGAATTCAGCCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTGCAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTAcc       >  2:725816/1‑140 (MQ=255)                                                                                                                              aTGAATTCAGCCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTGCAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTAcc       >  2:508832/1‑140 (MQ=255)                                                                                                                              aTGAATTCAGCCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTGCAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTAcc       >  1:682684/1‑140 (MQ=255)                                                                                                                                 aaTTCAGCCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTGCAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAAt    <  1:508832/140‑1 (MQ=255)                                                                                                                                   ttCAGCCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTGCAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGa  <  2:682684/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        CAGCGTAATCAGCGCGCAGAGTACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGTTTTCCATCGTGTTCAGCATAAAAGCCACGTGCCTGTTCAACGGTCAGGTGCAGCATTTTGGTGCCAACAATTTTGAACCCTGCAGCTTCAAAGCGCGCAAAGATATTACCAATGA  >  NC_000913/2644540‑2644809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑