Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,687,452	T→C	intergenic (‑44/+17)	glnB ← / ← glrR	nitrogen regulatory protein PII‑1/DNA‑binding transcriptional activator GlrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,687,452	0	T	C	100.0%	90.2 / NA	27	intergenic (‑44/+17)	glnB/glrR	nitrogen regulatory protein PII‑1/DNA‑binding transcriptional activator GlrR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/12);  total (15/12)

GTAATACCGACTTCGGCCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGTAACACATAACGCCAATTCATTCCTTGAAATCGTTTGCATCCAGCTCGTGTCGGGAAAGCAGTTTATAAAATTCTGTCCGGTTGCGCCCCGCCATTCTCGCCGCGTGGGTGACGTTGCCTTTGGTGATTTGCAGCAG  >  NC_000913/2687332‑2687588                                                                                                                         |                                                                                                                                         gTAATACCGACTTCGGCCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGCAACACATAACGCCAattca                                                                                                                       <  2:402737/140‑1 (MQ=255)                gCCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGCAACACATAACGCCAATTCATTCCTTGAAATCGtt                                                                                                        <  2:797944/140‑1 (MQ=255)                gCCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGCAACACATAACGCCAATTCATTCCTTGAAATCGtt                                                                                                        <  1:560216/140‑1 (MQ=255)                          cgcgGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGCAACACATAACGCCAATTCATTCCTTGAAATCGTTTGCATCCAGc                                                                                              >  2:361535/1‑140 (MQ=255)                           gcgGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGCAACACATAACGCCAATTCATTCCTTGAAATCGTTTGCATCCAGCt                                                                                             <  1:361535/140‑1 (MQ=255)                                cATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGCAACACATAACGCCAATTCATTCCTTGAAATCGTTTGCATCCAGCTCgtgt                                                                                        <  1:182400/140‑1 (MQ=255)                                                           tCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGCAACACATAACGCCAATTCATTCCTTGAAATCGTTTGCATCCAGCTCGTGTCGGGAAAGCAGTTTATAAAATTCTGTc                                                             <  1:100796/140‑1 (MQ=255)                                                            cGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGCAACACATAACGCCAATTCATTCCTTGAAATCGTTTGCATCCAGCTCGTGTCGGGAAAGCAGTTTATAAAATTCTGTcc                                                            >  2:318863/1‑140 (MQ=255)                                                             gCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGCAACACATAACGCCAATTCATTCCTTGAAATCGTTTGCATCCAGCTCGTGTCGGGAAAGCAGTTTATAAAATTCTGTCCg                                                           >  2:384970/1‑140 (MQ=255)                                                                     cTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGCAACACATAACGCCAATTCATTCCTTGAAATCGTTTGCATCCAGCTCGTGTCGGGAAAGCAGTTTATAAAATTCTGTCCGGTTGCGcc                                                   <  1:318863/140‑1 (MQ=255)                                                                             tGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGCAACACATAACGCCAATTCATTCCTTGAAATCGTTTGCATCCAGCTCGTGTCGGGAAAGCAGTTTATAAAATTCTGTCCGGTTGCGCCCCGCCAtt                                           >  1:95441/1‑140 (MQ=255)                                                                              gCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGCAACACATAACGCCAATTCATTCCTTGAAATCGTTTGCATCCAGCTCGTGTCGGGAAAGCAGTTTATAAAATTCTGTCCGGTTGCGCCCCGCCATtc                                          >  1:733586/1‑140 (MQ=255)                                                                                  ttCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGCAACACATAACGCCAATTCATTCCTTGAAATCGTTTGCATCCAGCTCGTGTCGGGAAAGCAGTTTATAAAATTCTGTCCGGTTGCGCCCCGCCATTCTcgc                                      <  2:924086/140‑1 (MQ=255)                                                                                     cTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGCAACACATAACGCCAATTCATTCCTTGAAATCGTTTGCATCCAGCTCGTGTCGGGAAAGCAGTTTATAAAATTCTGTCCGGTTGCGCCCCGCCATTCTcgccgc                                   <  2:95441/140‑1 (MQ=255)                                                                                         aaaaGGTCGCCTGTCTTTTGATCTGCTAAACGCAACACATAACGCCAATTCATTCCTTGAAATCGTTTGCATCCAGCTCGTGTCGGGAAAGCAGTTTATAAAATTCTGTCCGGTTGCGCCCCGCCATTCTCGCCGCGTg                                >  1:1089529/1‑139 (MQ=255)                                                                                         aaaaGGTCGCCTGTCTTTTGATCTGCTAAACGCAACACATAACGCCAATTCATTCCTTGAAATCGTTTGCATCCAGCTCGTGTCGGGAAAGCAGTTTATAAAATTCTGTCCGGTTGCGCCCCGCCATTCTCGCCGCGTg                                <  2:1089529/139‑1 (MQ=255)                                                                                              gTCGCCTGTCTTTTGATCTGCTAAACGCAACACATAACGCCAATTCATTCCTTGAAATCGTTTGCATCCAGCTCGTGTCGGGAAAGCAGTTTATAAAATTCTGTCCGGTTGCGCCCCGCCATTCTCGCCGCGTGGGTGAc                          >  2:780665/1‑140 (MQ=255)                                                                                              gTCGCCTGTCTTTTGATCTGCTAAACGCAACACATAACGCCAATTCATTCCTTGAAATCGTTTGCATCCAGCTCGTGTCGGGAAAGCAGTTTATAAAATTCTGTCCGGTTGCGCCCCGCCATTCTCGCCGCGTGGGTGAc                          >  1:164812/1‑140 (MQ=255)                                                                                               tCGCCTGTCTTTTGATCTGCTAAACGCAACACATAACGCCAATTCATTCCTTGAAATCGTTTGCATCCAGCTCGTGTCGGGAAAGCAGTTTATAAAATTCTGTCCGGTTGCGCCCCGCCATTCTCGCCGCGTGGGTGACg                         >  2:831354/1‑140 (MQ=255)                                                                                                 gCCTGTCTTTTGATCTGCTAAACGCAACACATAACGCCAATTCATTCCTTGAAATCGTTTGCATCCAGCTCGTGTCGGGAAAGCAGTTTATAAAATTCTGTCCGGTTGCGCCCCGCCATTCTCGCCGCGTGGGTGACGtt                       <  1:384970/140‑1 (MQ=255)                                                                                                   cTGTCTTTTGATCTGCTAAACGCAACACATAACGCCAATTCATTCCTTGAAATCGTTTGCATCCAGCTCGTGTCGGGAAAGCAGTTTATAAAATTCTGTCCGGTTGCGCCCCGCCATTCTCGCCGCGTGGGTGACGTTGc                     >  2:290471/1‑140 (MQ=255)                                                                                                      tCTTTTGATCTGCTAAACGCAACACATAACGCCAATTCATTCCTTGAAATCGTTTGCATCCAGCTCGTGTCGGGAAAGCAGTTTATAAAATTCTGTCCGGTTGCGCCCCGCCATTCTCGCCGCGTGGGTGACGTTGCCtt                  >  1:63671/1‑140 (MQ=255)                                                                                                        ttttGATCTGCTAAACGCAACACATAACGCCAATTCATTCCTTGAAATCGTTTGCATCCAGCTCGTGTCGGGAAAGCAGTTTATAAAATTCTGTCCGGTTGCGCCCCGCCATTCTCGCCGCGTGGGTGACGTTGCCTTTg                >  2:959193/1‑140 (MQ=255)                                                                                                           tGATCTGCTAAACGCAACACATAACGCCAATTCATTCCTTGAAATCGTTTGCATCCAGCTCGTGTCGGGAAAGCAGTTTATAAAATTCTGTCCGGTTGCGCCCCGCCATTCTCGCCGCGTGGGTGACGTTGCCTTTGGTg             <  1:959193/140‑1 (MQ=255)                                                                                                                tGCTAAACGCAACACATAACGCCAATTCATTCCTTGAAATCGTTTGCATCCAGCTCGTGTCGGGAAAGCAGTTTATAAAATTCTGTCCGGTTGCGCCCCGCCATTCTCGCCGCGTGGGTGACGTTGCCTTTGGTGATTTg        >  1:1090005/1‑140 (MQ=255)                                                                                                                  cTAAACGCAACACATAACGCCAATTCATTCCTTGAAATCGTTTGCATCCAGCTCGTGTCGGGAAAGCAGTTTATAAAATTCTGTCCGGTTGCGCCCCGCCATTCTCGCCGCGTGGGTGACGTTGCCTTTGGTGATTTgca      >  1:709706/1‑140 (MQ=255)                                                                                                                  cTAAACGCAACACATAACGCCAATTCATTCCTTGAAATCGTTTGCATCCAGCTCGTGTCGGGAAAGCAGTTTATAAAATTCTGTCCGGTTGCGCCCCGCCATTCTCGCCGCGTGGGTGACGTTGCCTTTGGTGATTTgca      >  1:557774/1‑140 (MQ=255)                                                                                                                   tAAACGCAACACATAACGCCAATTCATTCCTTGAAATCGTTTGCATCCAGCTCGTGTCGGGAAAGCAGTTTATAAAATTCTGTCCGGTTGCGCCCCGCCATTCTCGCCGCGTGGGTGACGTTGCCTTTGGTGATTTgcag     <  2:557774/140‑1 (MQ=255)                                                                                                                      aCGCAACACATAACGCCAATTCATTCCTTGAAATCGTTTGCATCCAGCTCGTGTCGGGAAAGCAGTTTATAAAATTCTGTCCGGTTGCGCCCCGCCATTCTCGCCGCGTGGGTGACGTTGCCTTTGGTGATTTGcagcag  <  1:780665/140‑1 (MQ=255)                                                                                                                         |                                                                                                                                         GTAATACCGACTTCGGCCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGTAACACATAACGCCAATTCATTCCTTGAAATCGTTTGCATCCAGCTCGTGTCGGGAAAGCAGTTTATAAAATTCTGTCCGGTTGCGCCCCGCCATTCTCGCCGCGTGGGTGACGTTGCCTTTGGTGATTTGCAGCAG  >  NC_000913/2687332‑2687588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑