Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,731,729	A→G	R815R (CGT→CGC)	clpB ←	ClpB chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,731,729	0	A	G	100.0%	288.0 / NA	79	R815R (CGT→CGC)	clpB	ClpB chaperone
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (41/38);  total (41/38)

TTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGACGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTACAGACGTTTCAACTGAATCTGCGCAATCGAGGCAATGT  >  NC_000913/2731593‑2731865                                                                                                                                         |                                                                                                                                         ttATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGt                                                                                                                                       <  1:998327/140‑1 (MQ=255)  tATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTg                                                                                                                                      <  1:738634/140‑1 (MQ=255)  tATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACACTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTg                                                                                                                                      <  2:756971/140‑1 (MQ=255)      aTTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCAcc                                                                                                                                  <  2:552228/140‑1 (MQ=255)          aCTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCa                                                                                                                                    >  1:865358/1‑134 (MQ=255)          aCTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCa                                                                                                                                    <  2:865358/134‑1 (MQ=255)          aCTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAg                                                                                                                              >  2:951013/1‑140 (MQ=255)          aCTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAg                                                                                                                              >  2:1045416/1‑140 (MQ=255)              gACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCg                                                                                                                          <  2:786955/140‑1 (MQ=255)              gACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCg                                                                                                                          <  1:951013/140‑1 (MQ=255)                cGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTg                                                                                                                                      >  2:240186/1‑126 (MQ=255)                cGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTg                                                                                                                                      <  1:240186/126‑1 (MQ=255)                cGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCAcc                                                                                                                                  <  2:848819/130‑1 (MQ=255)                cGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCAcc                                                                                                                                  >  1:848819/1‑130 (MQ=255)                cGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGa                                                                                                                        >  2:652239/1‑140 (MQ=255)                 ggCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGTGTGCACCATAGACCGGAt                                                                                                                       >  2:479765/1‑140 (MQ=255)                 ggCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCg                                                                                                                          <  1:997917/137‑1 (MQ=255)                 ggCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCg                                                                                                                          >  2:997917/1‑137 (MQ=255)                  gCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATc                                                                                                                      >  2:674921/1‑140 (MQ=255)                   cGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCg                                                                                                                     >  1:853339/1‑140 (MQ=255)                      cAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTaa                                                                                                                  <  2:1040769/140‑1 (MQ=255)                      cAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTaa                                                                                                                  <  2:770244/140‑1 (MQ=255)                       aaTCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAAc                                                                                                                 <  1:652239/140‑1 (MQ=255)                                   aTTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTca                                                                                                     >  2:404193/1‑140 (MQ=255)                                       aCTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGGGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTcagcag                                                                                                 >  2:755328/1‑140 (MQ=255)                                           ccAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTc                                                                                             <  1:1045416/140‑1 (MQ=255)                                            cAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTcagc                                                                                                   >  2:565710/1‑133 (MQ=255)                                            cAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTcagc                                                                                                   <  1:565710/133‑1 (MQ=255)                                             aGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAg                                                                                           >  2:1006846/1‑140 (MQ=255)                                                cGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAgcgc                                                                                        <  2:853339/140‑1 (MQ=255)                                                cGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAgcgc                                                                                        <  1:61774/140‑1 (MQ=255)                                                 gAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGcc                                                                                       >  1:301619/1‑140 (MQ=255)                                                     cACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGt                                                                                   <  1:674921/140‑1 (MQ=255)                                                               ggAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAAtgtg                                                                         <  1:348501/140‑1 (MQ=255)                                                                 aaCCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGa                                                                       >  2:661606/1‑140 (MQ=255)                                                                 aaCCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGa                                                                       >  2:393402/1‑140 (MQ=255)                                                                  aCCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACC‑‑AGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGAttt                                                                    >  1:312331/1‑140 (MQ=255)                                                                    cAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGAttt                                                                    <  1:661606/140‑1 (MQ=255)                                                                      aTTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCa                                                                  >  1:966070/1‑140 (MQ=255)                                                                      aTTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCa                                                                  >  1:779464/1‑140 (MQ=255)                                                                      aTTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCa                                                                  >  2:228061/1‑140 (MQ=255)                                                                           ccAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAAcc                                                             >  2:834467/1‑140 (MQ=255)                                                                              gACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACg                                                          <  1:802447/140‑1 (MQ=255)                                                                               aCAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTAGATTTCATAACCACGt                                                         >  1:837375/1‑140 (MQ=255)                                                                                 aGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGttc                                                       >  2:299259/1‑140 (MQ=255)                                                                                      ttGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACC‑‑AGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGa                                                <  2:312331/140‑1 (MQ=255)                                                                                         cTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGAc                                               <  1:1006846/140‑1 (MQ=255)                                                                                           gtgCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGt                                             >  1:1041993/1‑140 (MQ=255)                                                                                           gtgCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGt                                             <  1:299259/140‑1 (MQ=255)                                                                                             gCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACg                                                          >  1:638648/1‑125 (MQ=255)                                                                                             gCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACg                                                          <  2:638648/125‑1 (MQ=255)                                                                                             gCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGttt                                           >  2:694445/1‑140 (MQ=255)                                                                                             gCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGttt                                           >  1:494516/1‑140 (MQ=255)                                                                                             gCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGttt                                           >  1:638184/1‑140 (MQ=255)                                                                                                  cGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTACa                                      <  2:494516/140‑1 (MQ=255)                                                                                                    ggTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTACAGa                                    <  1:834467/140‑1 (MQ=255)                                                                                                      ttttCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTACAGACg                                  >  2:1066565/1‑140 (MQ=255)                                                                                                        ttCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTACAGACGtt                                <  1:1066565/140‑1 (MQ=255)                                                                                                        ttCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTACAGACGtt                                <  2:779464/140‑1 (MQ=255)                                                                                                        ttCGATCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTACAGACGtt                                <  1:228061/140‑1 (MQ=255)                                                                                                            aTCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTACAGACGTTTCaa                            >  2:993257/1‑140 (MQ=255)                                                                                                            aTCTGCTGCTGAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTACAGACGTTTCaa                            >  1:1065714/1‑140 (MQ=255)                                                                                                               tgctgctgAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTACAGACGTTTCAACTg                         <  2:617118/140‑1 (MQ=255)                                                                                                               tgctgctgAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTACAGACGTTTCAACTg                         >  2:442173/1‑140 (MQ=255)                                                                                                               tgctgctgAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTACAGACGTTTCAACTg                         >  2:780480/1‑140 (MQ=255)                                                                                                                      gAATTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTACAGACGTTTCAACTGAATCTgc                  <  2:1041993/140‑1 (MQ=255)                                                                                                                       aaTTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAGTGTGGATTTTATAACCACGTTCTTCCAGACGTTTGTACAGACGATTCAACTGAATCTgcg                 >  2:979838/1‑140 (MQ=255)                                                                                                                       aaTTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTACAGACGTTTCAACTGAATCTgcg                 >  2:602138/1‑140 (MQ=255)                                                                                                                       aaTTGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTACAGACGTTTCAACTGAATCTgcg                 >  2:38485/1‑140 (MQ=255)                                                                                                                         ttGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCGTCCAGACGTTTGTACAGACGTTTCAACTGAATCTGCGCa               >  1:437039/1‑140 (MQ=255)                                                                                                                          tGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTACAGACGTTTCAACTGAATCTGCGCaa              <  1:51526/140‑1 (MQ=255)                                                                                                                          tGCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTACAGACGTTTCAACTGAATCTGCGCaa              <  2:837375/140‑1 (MQ=255)                                                                                                                           gCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTACAGACGTTTCAACTGAATCTGCGCAAt             <  1:755328/140‑1 (MQ=255)                                                                                                                           gCACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTACAGACGTTTCAACTGAATCTGCGCAAt             >  1:41302/1‑140 (MQ=255)                                                                                                                            cACGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTACAGACGTTTCAACTGAATCTGCGCAATc            >  2:289074/1‑140 (MQ=255)                                                                                                                              cGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTACAGACGTTTCAACTGAATCTGCGCaa              <  2:575743/136‑1 (MQ=255)                                                                                                                              cGTTTCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTACAGACGTTTCAACTGAATCTGCGCaa              >  1:575743/1‑136 (MQ=255)                                                                                                                                  tCAGAGGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTACAGACGTTTCAACTGAATCTGCGCAATCGAGGCa      <  1:780480/140‑1 (MQ=255)                                                                                                                                      agGGCGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTACAGACGTTTCAACTGAATCTGCGCAATCGAGGCAAtgt  <  1:393402/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         TTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGACGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTACAGACGTTTCAACTGAATCTGCGCAATCGAGGCAATGT  >  NC_000913/2731593‑2731865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑