Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,799,453	T→C	V97A (GTG→GCG)	alaE →	L‑alanine exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,799,453	0	T	C	100.0%	250.2 / NA	69	V97A (GTG→GCG)	alaE	L‑alanine exporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/34);  total (35/34)

AACATCTTAATTGCATGGCCATACGGTATGTACCGTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAG  >  NC_000913/2799317‑2799588                                                                                                                                         |                                                                                                                                        aaCATCTTAATTGCATGGCCATACGGTATGTACCGTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGc                                                                                                                                      <  2:313450/140‑1 (MQ=255)    aTCTTAATTGCATGGCCATACGGTATGTACCGTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGAt                                                                                                                                   <  1:268550/140‑1 (MQ=255)      cTTAATTGCATGGCCATACGGTATGTACCGTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCt                                                                                                                                 <  2:734478/140‑1 (MQ=255)                tGGCCATACGGTATGTACCGTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGAt                                                                                                                                   <  2:267710/128‑1 (MQ=255)                tGGCCATACGGTATGTACCGTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGAt                                                                                                                                   >  1:267710/1‑128 (MQ=255)                tGGCCATACGGTATGTACCGTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAgtggt                                                                                                                       >  2:366564/1‑140 (MQ=255)                tGGCCATACGGTATGTACCGTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAgtggt                                                                                                                       >  1:171005/1‑140 (MQ=255)                tGGCCATACGGTATGTACCGTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAgtggt                                                                                                                       >  1:759954/1‑140 (MQ=255)                     aTACGGTATGTACCGTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGgcg                                                                                                                  <  1:6171/140‑1 (MQ=255)                      tACGGTATGTACCGTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGgcgc                                                                                                                 >  1:742738/1‑140 (MQ=255)                          gTATGTACCGTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGAc                                                                                                             <  1:264183/140‑1 (MQ=255)                           tATGTACCGTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACt                                                                                                            <  1:336123/140‑1 (MQ=255)                           tATGTACCGTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACt                                                                                                            <  1:366564/140‑1 (MQ=255)                             tGTACCGTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTgg                                                                                                          >  1:13367/1‑140 (MQ=255)                               tACCGTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCa                                                                                                        >  1:788909/1‑140 (MQ=255)                               tACCGTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCa                                                                                                        >  1:66788/1‑140 (MQ=255)                                aCCGTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCAt                                                                                                       <  2:742738/140‑1 (MQ=255)                                aCCGTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCAt                                                                                                       >  1:792151/1‑140 (MQ=255)                                  cGTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCa                                                                                                        >  2:656527/1‑137 (MQ=255)                                  cGTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCa                                                                                                        <  1:656527/137‑1 (MQ=255)                                  cGTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCa                                                                                                     >  1:1086785/1‑140 (MQ=255)                                   gTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAg                                                                                                    >  1:504919/1‑140 (MQ=255)                                   gTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAg                                                                                                    >  1:689484/1‑140 (MQ=255)                                   gTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAg                                                                                                    >  2:169475/1‑140 (MQ=255)                                   gTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAg                                                                                                    >  1:126943/1‑140 (MQ=255)                                    tGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGa                                                                                                   >  2:868495/1‑140 (MQ=255)                                     gATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGa                                                                                                              >  1:648299/1‑128 (MQ=255)                                     gATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGa                                                                                                              >  1:520807/1‑128 (MQ=255)                                     gATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGa                                                                                                              <  2:648299/128‑1 (MQ=255)                                     gATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGa                                                                                                              <  2:520807/128‑1 (MQ=255)                                     gATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGAt                                                                                                  >  1:301642/1‑140 (MQ=255)                                       tCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTa                                                                                                <  1:169475/140‑1 (MQ=255)                                         tGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCa                                                                                                        >  2:60714/1‑130 (MQ=255)                                         tGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCa                                                                                                        <  1:60714/130‑1 (MQ=255)                                                gcgcgcGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGcggcgg                                                                                       <  2:574068/140‑1 (MQ=255)                                                gcgcgcGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGcggcgg                                                                                       <  2:792151/140‑1 (MQ=255)                                                 cgcgcgGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGCGGCGGt                                                                                      >  2:423049/1‑140 (MQ=255)                                                 cgcgcgGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGCGGCGGt                                                                                      <  2:1086785/140‑1 (MQ=255)                                                  gcgcgGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGCGGCGGTc                                                                                     <  1:868495/140‑1 (MQ=255)                                                  gcgcgGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCATATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGCGGCGGTc                                                                                     <  2:494936/140‑1 (MQ=255)                                                    gcgGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGCGGCGGTCAg                                                                                   <  1:423049/140‑1 (MQ=255)                                                     cgGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGa                                                                                                   >  2:798935/1‑123 (MQ=255)                                                     cgGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGa                                                                                                   <  1:798935/123‑1 (MQ=255)                                                     cgGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATggcg                                                                                           <  2:480231/131‑1 (MQ=255)                                                     cgGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATggcg                                                                                           >  1:480231/1‑131 (MQ=255)                                                     cgGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGCGGCGGTCAGt                                                                                  >  2:852923/1‑140 (MQ=255)                                                     cgGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGCGGCGGTCAGt                                                                                  >  1:846021/1‑140 (MQ=255)                                                     cgGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGCGGCGGTCAGt                                                                                  <  2:476587/140‑1 (MQ=255)                                                     cgGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGCGGCGGTCAGt                                                                                  >  1:794878/1‑140 (MQ=255)                                                                gTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGCGGCGGTCAGTTCAAACATCgt                                                                       <  2:171005/140‑1 (MQ=255)                                                                     cccGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGCGGCGGTCAGTTCAAACATCGTTGttt                                                                  <  2:868019/140‑1 (MQ=255)                                                                     cccGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGCGGCGGTCAGTTCAAACATCGTTGttt                                                                  <  1:558045/140‑1 (MQ=255)                                                                     cccGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGCATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGCGGCGGTTAGTTCAAACATCGTTGttt                                                                  >  1:150436/1‑140 (MQ=255)                                                                         tCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGCGGCGGTCAGTTCAAACATCGTTGTTTCGAt                                                              >  1:939202/1‑140 (MQ=255)                                                                          cGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATg                                                             <  2:504919/140‑1 (MQ=255)                                                                           gggCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGt                                                            >  2:911047/1‑140 (MQ=255)                                                                                          tCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGttt                                             <  2:846021/140‑1 (MQ=255)                                                                                           cTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGCGGCGGTCAGTTCAAACATCGCTGTTTCGATGTTTGTGGGGGCGGgttt                                            >  2:647477/1‑139 (MQ=255)                                                                                                             tATGTGACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGatta                          >  1:500531/1‑140 (MQ=255)                                                                                                               tgtgACGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGattatt                        >  1:933901/1‑140 (MQ=255)                                                                                                                    cGTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTgccgc                   <  2:933901/140‑1 (MQ=255)                                                                                                                     gTTCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTgccgcc                  >  2:205944/1‑140 (MQ=255)                                                                                                                      ttCCAGTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGccgccg                 <  1:911047/140‑1 (MQ=255)                                                                                                                           gTCACCGGTGTATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGt            >  2:836411/1‑140 (MQ=255)                                                                                                                                  gtgtATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGt     <  1:205944/140‑1 (MQ=255)                                                                                                                                  gtgtATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGt     <  1:852923/140‑1 (MQ=255)                                                                                                                                    gtATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCa   >  1:510735/1‑140 (MQ=255)                                                                                                                                     tATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAg  <  2:13367/140‑1 (MQ=255)                                                                                                                                     tATGCGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAg  <  2:126943/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        AACATCTTAATTGCATGGCCATACGGTATGTACCGTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAG  >  NC_000913/2799317‑2799588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑